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  • 1
    ISSN: 1432-1211
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The mouse lymphocyte surface alloantigen, Ly-31, defined by monoclonal antibody N1.10 (IgG2b, k) and controlled by a gene locus closely linked to theAkp-2 locus on chromosome 4, was biochemically investigated. By employing a quantitative immunoassay system, it was found that the Ly-31.1-specific antibody detected an allotypic determinant of mouse alkaline phosphatase. Ly-31.1, i. e., mouse alkaline phosphatase, was expressed predominantly in kidney and bone and was also detected in placenta, lung, and testis. Concerning tumor cell lines, they varied in the amount of antigen present, with both T and B lymphoid lineages selectively possessing the antigen. In normal lymphoid tissues, lesser amounts of antigen were detected. The binding of mouse alkaline phosphatase to Ly-31.1-specific monoclonal antibodies was specific in nature. The Ly-31.1 antigen was immunoprecipitated from the lysates of surface-radiolabeled YAC-1 moloney leukemia cells, and appeared as a single band of about 78 000 under both reduced and nonreduced conditions on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Furthermore, treatment of tumor cell lines with phosphatidylinositol-specific-phospholipase C resulted in the removal of Ly-31 antigen from the cell surface. These results suggest that a gene cluster containing theLy-31 andAkp-2 loci which control the alkaline phosphatase is formed on mouse chromosome 4. The Ly-31 antigen is the first enzyme demonstrated to be a lymphocyte surface alloantigen.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    College Park, Md. : American Institute of Physics (AIP)
    The Journal of Chemical Physics 90 (1989), S. 4835-4846 
    ISSN: 1089-7690
    Source: AIP Digital Archive
    Topics: Physics , Chemistry and Pharmacology
    Notes: Rotational transition in atom–diatom chemical reaction is theoretically studied. A new approximate theory (which we call IOS-DW approximation) is proposed on the basis of the physical idea that rotational transition in reaction is induced by the following two different mechanisms: rotationally inelastic half collision in both initial and final arrangement channels, and coordinate transformation in the reaction zone. This theory gives a fairy compact expression for the state-to-state transition probability. Introducing the additional physically reasonable assumption that reaction (particle rearrangement) takes place in a spatially localized region, we have reduced this expression into a simpler analytical form which can explicitly give overall rotational state distribution in reaction. Numerical application was made to the H+H2 reaction and demonstrated its effectiveness for the simplicity. A further simplified most naive approximation, i.e., independent events approximation was also proposed and demonstrated to work well in the test calculation of H+H2. The overall rotational state distribution is expressed simply by a product sum of the transition probabilities for the three consecutive processes in reaction: inelastic transition in the initial half collision, transition due to particle rearrangement, and inelastic transition in the final half collision.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1619-7089
    Keywords: Slant hole collimator ; Gamma camera ; Atraumatic rCBF measurement ; 133Xe
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The purpose of this study is to develop a useful gamma camera system for atraumatic rCBF measurement by the133Xe intravenous injection (i.v.) method. A super high sensitivity slant hole (SHS-SL) collimator was designed for this purpose. The gamma camera used with this collimator was able to move close to the patient's head and obtain satisfactory count rates (130 kcpm–178 kcpm in a hemisphere) with a low dosage (10 mCi) of133Xe. To validate this rCBF measurement system three studies were carried out: a comparative evaluation was performed against the flow values with the intracarotid133Xe injection (i.c.) method, reproducibility of flow values was assessed from two serial i.v. measurements, and flow values were compared with clinical severity of the patients. The comparative study showed good correlation in the hemispheric and regional values between the two methods;r values 0.98 and 0.64–0.93 for gray matter flow and 0.96 and 0.78–0.93 for initial slope index. In the reproducibility study, the variation coefficients in the hemispheric and regional values between the two serial measurements were 5.0% and 7.3%–14.2% for gray mater flow, 5.2% and 7.9%–12.9% for initial slope index. The reproducibility in this study was as good as those with the i.c. method reported previously. The hemispheric flow values from our gamma camera system correlated well with the clinical severity of cerebrovascular disease. These results show that the gamma camera system with the SHS-SL collimator is useful for atraumatic rCBF measurement.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Bulletin of environmental contamination and toxicology 41 (1988), S. 12-16 
    ISSN: 1432-0800
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Medicine
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Applied microbiology and biotechnology 31 (1989), S. 401-405 
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Magnetotactic bacteria were introduced into granulocytes and monocytes by phagocytosis. The number of phagocytes containing bacterial magnetites (magneto-sensitive cells) became constant after 1.5 h incubation, and viable phagocytes contained about 20–40 cells of magnetotactic bacteria. Granulocytes and monocytes containing bacterial magnetites were separated by magnet a Samarium-cobalt from lymphocytes. After separation, 89% of lymphocytes were recovered and 95% of the cells were viable. The contamination of phagocytes in the recovered lymphocytes was below 0.8%. Magneto-sensitive granulocytes and monocytes were removed by applying a magnetic field. The nitro-blue tetrazolium-reducing, chemotactic and phagocytic abilities of phagocytes ingesting magnetotactic bacteria were 84%, 88% and 87% respectively after 1 h incubation.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Basic research in cardiology 80 (1985), S. 417-429 
    ISSN: 1435-1803
    Keywords: microsphere ; blood flow ; Poisson distribution ; reference sample method
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary When microspheres are injected into the circulation, they are trapped in the arteriolar or capillary system within various organs. It has been confirmed in animal experiments that the number of microspheres in a myocardial sample approximately follows a Poisson distribution, under adequate experimental conditions. On the basis of this result, we arrived at the following hypothesis: When regional blood flow is measured under a steady state by the reference sample method, the 95% relative error can be approximated by $$ \pm 196\sqrt {1/v + 1w} $$ , wherev andw represent the number of microspheres in blood sample and myocardial sample, respectively. The equation is valid ifv andw are greater than 400 and 49, respectively. We obtained an expression of the relation among the percent of increase or decrease in regional blood flow being verified, the probability of increase or decrease, as a statistically significant variation and the number of microspheres in a myocardial sample. An investigator can work out an approximate experimental design using this expression. For instance, when the increase in regional blood flow required for verification is expected to be 20% and he wants this increase to be verified by 90%-probability and as a statistically significant increase, he can predict from this expression that the number of microspheres in a myocardial sample should be 472 and 567 before and after the experimental intervention on the coronary circulation, respectively. The expression is useful as index for experiments involving use of the microsphere method.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    International journal of infrared and millimeter waves 10 (1989), S. 907-930 
    ISSN: 1572-9559
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Two new types of infrared detectors have been developed for the precision earth sensor in the three axis stabilized satellite, Engineering Test Satellite VI (ETS-VI). Both detectors have a pair of infrared sensing elements, each of which is made of a pyroelectric material and mounted on an immersion lens. A-type element is a flake of lead titanate ceramic, and B-type is a sputtered epitaxial film of calcium-modified lead titanate. The precision earth sensor consists of a scanning mirror and an infrared telescope containing an objective lens, plus either type of infrared detector. Detectivity of each type at 120Hz, which is the nominal frequency of the earth sensor's signal, is 1.5×109Hz1/2/W(A-type) and 2.7×109Hz1/2/W(B-type) at 14∼16.25μm. Each value is 2.5 and 4.5 times as high as the previous model in ETS-V. A-type detector passed the space environmental test and the random error cf attitude measurement was 0.03 deg with the preliminary model of the earth sensor being ahead of the breadboard model. This measurement accuracy is nearly equal to that of the foremost earth sensor. A-type was chosen as the detector for ETS-VI. The random error of the earth sensor with B-type detector is 0.012deg, smaller than a half of the A-type sensor's error. Investigation of B-type is being continued in order to confirm its reliability for space use.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Astrophysics and space science 118 (1986), S. 283-286 
    ISSN: 1572-946X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The contact condition which includes the effect of pressure in common envelope is derived and employed in the evolutionary calculation of a close binary system in contact phase. The result indicates that the effect is negligibly small and the equality of surface potentials of both components is a sufficiently good approximation for the contact condition.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Astrophysics and space science 134 (1987), S. 219-234 
    ISSN: 1572-946X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The evolution of three close binary systems of total mass 20.4M ⊙ in and after the phase of mode Br mass-transfer in caseA of mass exchange is investigated. In every case a secondary component evolves to interfere with the progress of primary's evolution and the system overflows the outer critical surface before the primary completes its nuclear-burning evolution. This strongly indicates the importance of simultaneous calculation of both components. A summary of evolution of the systems considered in this series of papers up to the stage ofL 2-overflow is given. The observational aspects of the numerical models are also discussed.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Springer
    Astrophysics and space science 134 (1987), S. 161-176 
    ISSN: 1572-946X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We present in this paper the evolutionary characteristics of the systems belonging to two evolution types, designated as 1C2COf and 1Nc2COf, among seven evolution types which were studied in the preceding paper of this series. These two types are most complicated and interesting in the evolutionary behaviour, which consists of repeated detached, semi-detached, and contact phases. We discuss observational aspects of the results. Evolutionary behaviour of the systems are also discussed, compared with the thermal relaxation oscillation model.
    Type of Medium: Electronic Resource
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