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  • 1
    Electronic Resource
    Electronic Resource
    Flory's Viscosity Factor for the System Polystyrene + Cyclohexane at 34.5 °C (1980)
    s.l. : American Chemical Society
    Macromolecules 13 (1980), S. 588-592 
    Details
    ISSN: 1520-5835
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ma60075a021
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  • 2
    Electronic Resource
    Electronic Resource
    A plan for on-line production and acceleration of radioactive isotope beams by use of electron cyclotron resonance ion source NANOGAN : The 8th international conference on ion sources (2000)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 71 (2000), S. 777-779 
    Details
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: For the precise low background experiments on β decay and material sciences, we are planning to build a new ISOL-type facility to accelerate various β-radioactive nuclei utilizing the existing 5 MV Van de Graaff accelerator and an RFQ linac. Present status of the designing work is reported. In the present work, optical elements of the 41-m-long beam transport line to connect Van de Graaff accelerator and an RFQ linac were designed. Using two dipole magnets, two electric quadrupole doublets and six electric quadrupole triplets, low energy radioactive nuclear beams can be transported with sufficient efficiency. © 2000 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1150291
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  • 3
    Electronic Resource
    Electronic Resource
    Dentoalveolar reconstruction of a missing premaxilla using bone graft and endosteal implants (2003)
    Oxford, UK : Blackwell Science Ltd
    Journal of oral rehabilitation 30 (2003), S. 0 
    Details
    ISSN: 1365-2842
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: summary  We report here on a patient with bilateral cleft lip and palate (BCLP) and a missing premaxilla, who underwent dentoalveolar reconstruction of the cleft and premaxillary alveolus using endosteal implants after bone grafting. The patient, whose maxillary incisors and premaxilla were missing, had corticocancellous bone grafting from the iliac crest, followed by excellent bone formation at the anterior alveolus. After the placement of the endosteal implants and the completion of the pre-surgical orthodontic alignment, orthognathic surgery was performed for the restoration of a Class III open bite. After post-operative orthodontic preparation, the final fixed prostheses were completed. This treatment procedure offers an option of dentoalveolar reconstruction for BCLP patients with an excised premaxilla.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2842.2003.00999.x
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  • 4
    Electronic Resource
    Electronic Resource
    Dental rehabilitation using endosseous implants and orthognathic surgery in patients with cleft lip and palate: report of two cases (2000)
    Oxford, UK : Blackwell Science Ltd
    Journal of oral rehabilitation 27 (2000), S. 0 
    Details
    ISSN: 1365-2842
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: We describe the use of endosseous implants in the autogenous particulate cancellous bone and marrow grafted alveoli after orthognathic surgery for dental rehabilitation of patients with cleft lip and palate. This procedure has been applied to two patients and produced good results functionally and aesthetically. The results are encouraging and indicate that implant placement after orthognathic surgery is useful for patients both with congenital missing teeth and retrognathic maxillae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2842.2000.00539.x
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  • 5
    Electronic Resource
    Electronic Resource
    A time pickup system for monitoring cyclotron beam bursts (2002)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 73 (2002), S. 51-55 
    Details
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: We have developed a time pickup system for monitoring the beam bursts from a cyclotron. The time pickup can measure the phase and the width of the beam bursts by detecting secondary electrons emitted from a foil or a wire target inserted into the beam with time resolutions around 200 ps full width at half maximum in spite of its simple structure. By using the foil and the wire targets appropriately, monitoring the bursts of a variety of beams can be achieved. In addition, by adjusting the position of the target, a high enough detection rate of the beam burst is obtained with a minimum of interference with the beam. The present investigation shows that it is needed to adjust the collection voltage of the time pickup because the optimum collection voltages for high resolution measurement are in the range from −0.5 to −1.0 kV and those for high efficiency measurement are not less than −4.0 kV. © 2002 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1416116
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  • 6
    Electronic Resource
    Electronic Resource
    Studies on the binding mechanism of fluoroquinolones to melanin (2000)
    Springer
    Journal of infection and chemotherapy 6 (2000), S. 72-76 
    Details
    ISSN: 1437-7780
    Keywords: Key words Ocular melanin ; Melanin affinity ; Fluoroquinolones ; Metal ions ; Binding mechanism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In order to elucidate the binding mechanism of melanin and fluoroquinolones (ofloxacin, norfloxacin, ciprofloxacin, lomefloxacin, levofloxacin), we investigated the interaction of fluoroquinolones with compounds such as l-β-(3,4-dihydroxyphenyl) alanine (l-DOPA), l-tyrosine, 5-hydroxy-l-tryptophan, l-tryptophan, and l-phenylalanine, which possess the kind of functional groups that melanin does and are closely related to melanin. The recovery of drugs from the melanin-drug complexes by metal ions of Li, Na, Ka, Mg, Ca, Ba, Cu, Ni, and Fe was demonstrated. Smaller and highly charged cations were found to be more effective for this recovery, and magnesium ions were the most effective of all the ions investigated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00012154
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  • 7
    Electronic Resource
    Electronic Resource
    Cytoflurometric nuclear DNA-determinations in infant, adolescent, adult and aging human hearts (1983)
    Springer
    Histochemistry and cell biology 77 (1983), S. 485-494 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The progress of polyploidization in the human heart muscle cell was investigated by cytofluorometry, involving selective measurements of heart muscle cell nuclei. Thirty-two tissue samples, taken from the free wall of the left ventricle of each autopsied heart, were fixed in Carnoy's fluid. From thick (100–150 μm) paraffin sections, isolated cells for smears were obtained by enzyme digestion and ultrasonic treatment. The smears were stained with azocarmin G to eliminate background fluorescence and subsequently stained by an acriflavine-Feulgen reaction. Cytofluorometric DNA-determinations were carried out selectively on heart muscle cell nuclei, using the muscle striations revealed by azocarmin G-fluorescence as specific markers. The dynamic process of polyploidization in normal hearts could be divided into four stages. In the first stage (under 1 year of age), almost all heart muscle cell nuclei (94.3±1.8%) were diploid. In the second stage (1 to 9 years of age), the number of tetraploid nuclei increased (13.6±7.1%). In the third stage (9 to 22 years of age), octaploid nuclei first appeared and the number of tetraploid nuclei increased (26.7±3.9%). The DNA pattern in the fourth stage (22 to 75 years of age) was relatively constant, with a ratio of diploid (62.4±8.7%), tetraploid (31.4±6.7%) and octaploid (5.8±3.9%) nuclei. From these results it was concluded that physiological polyploidization progresses in proportion to the increase of heart weight. The frequency of polyploid nuclei in human heart was not so high as resported by previous investigators.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00495803
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  • 8
    Electronic Resource
    Electronic Resource
    Fluorescence fading and stabilization in cytofluorometry (1980)
    Springer
    Histochemistry and cell biology 65 (1980), S. 269-276 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The factors affecting fluorescence fading in cytofluorometry were investigated using different kinds of nuclear staining, mounting media, and procedure of specimen preparation. Acceleration of fluorescence fading was observed in smear specimens treated with RNase, trypsin, or hypotonic solution before pararosaniline Feulgen nuclear staining. Similar effect was found for other DNA-stainings such as “33258 Hoechst” and Feulgen reactions with different Schiff-type dyes, such as acriflavine-SO2 and cresylviolet-SO2, when chemically pure DNA was used. Fluorescence decay was rapid for all fluorochromes examined, when glycerin or buffer solution was used as mounting medium. Marked stabilization of fluorescence emission was induced in specimen mounted in non-fluorescent resin, Entellan (Merck), after post-staining fixation with absolute methanol for all tested fluorochromes. The same treatment induced almost complete fluorescence stabilization of fluorescein isothiocyanate (FITC); no detectable fluorescence fading was observed in a specimen stained with indirect immunofluorescence reaction using anti-UV-DNA antibody, during storage for 2 years at room temperature without special protection against light. These observations suggest that factors which bring about conformational stability of macromoleculedye complexes generally induce fluorescence stabilization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00493176
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  • 9
    Electronic Resource
    Electronic Resource
    Cytofluorometry on cells isolated from paraffin sections after blocking of the background fluorescence by azocarmin G (1981)
    Springer
    Histochemistry and cell biology 71 (1981), S. 161-170 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A technique for isolation of cells from paraffin embedded tissue is indispensable for the performance of Feulgen-DNA cytofluorometry in parallel with the definition of histological characteristics. Background fluorescence due to nonspecific dye-binding by a “pseudo-plasmal reaction” is usually found to be so intense on cells isolated from formalin-fixed tissues, that we are often forced to abandon quantitative DNA determinations. In the present work, we report that fixation of tissues with Carnoy's fixative for 12 h at 5° C not only reduces nonspecific dye-binding but also facilitates the process of cell isolation. Furthermore, we find that pre-treatment of cells isolated from Carnoy-fixed tissues with acidic azocarmin G solution completely blocks nonspecific dye-binding in subsequent acriflavine Feulgen nuclear staining. This combination of techniques for specimen preparation enables us to carry out Feulgen-DNA cytofluorometry on cells isolated from histological sections with satisfactorily low coefficients of variation (less than 8%). The techniques should be widely applicable for parallel DNA determinations and histology.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00507820
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  • 10
    Electronic Resource
    Electronic Resource
    Nonspecific (“pseudo-plasmal”) dye-binding in the Feulgen nuclear stain and its blocking by azocarmin G (1980)
    Springer
    Histochemistry and cell biology 66 (1980), S. 169-180 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In Feulgen nuclear staining nonspecific dye-binding due to the “pseudo-plasmal reaction” is intensified in isolated cells with intact cytoplasm, and cannot be eliminated by the post-irradiation method. Fluorescence intensity in the cytoplasm sometimes exceeds that of specific nuclear fluorescence, especially in brain and heart muscle cells, and it was almost impossible to perform cytofluorometric DNA quantification on such specimens. Various kinds of aldehyde-blocking agents such as sodium borohydride, 2,4-dinitrophenylhydrazine, aniline, and sodium pyrosulfite were effective in reducing the “pseudo-plasmal reaction”. But the blocking effects were not complete because of additional release of reactive aldehyde groups during subsequent Feulgen hydrolysis. Acidic azocarmin G produces a complete block of all “pseudo-plasmal reaction” in acriflavine-Feulgen nuclear staining, allowing accurate DNA-cytofluorometry to be carried out.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00494643
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