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  • 1
    Electronic Resource
    Electronic Resource
    Immunologic Recognition of cell surface antigens in normal mouse neural tissues and in neuroepithelial cells of the OTT-6050 mouse teratoma (1982)
    Springer
    Acta neuropathologica 56 (1982), S. 214-224 
    Details
    ISSN: 1432-0533
    Keywords: Neural cell surface antigens ; Neural differentiation ; Mouse teratoma ; Radioimmune assay ; Immunoperoxidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A rabbit antiserum against mouse neonatal brain cell surface membranes labeled by immunoperoxidase (PAP) the cells of the central and peripheral nervous systems of adult and neonatal mice and their processes, as well as the differentiating neuroepithelial cells of three OTT-6050 mouse teratoma-derived tumors. Indirect immunofluorescence on living 14-day-old monolayer cultures of neonatal mouse brain demonstrated reaction of the immune serum with external surface membrane antigens of neuroblasts and of primitive and mature glial cells. Radioimmune assays (RIA) showed almost complete loss of antiserum binding to neonatal mouse brain plasma membranes after absorption with adult or neonatal mouse brain membranes, and no loss of binding after absorption by liver, spleen, kidney, and heart membranes. Cross-reactivity of the immune serum to several non-neural cell types was demonstrated by immunoperoxidase on sperm and sperm-precursors, on moderate numbers of epithelial cells in the medulla of adult mouse thymus, and, in the neonate, on a range of mesenchymal cells. This cross-reactivity was reflected in the RIA by a moderate reduction of immune serum binding to neonatal mouse brain plasma membranes after absorption with testis pellets and with thymus membranes. PAP staining showed loss of crossreactivity after testis or thymus absorption, without climination of neural cell recognition. Absorption with adult or neonatal mouse brain eliminated cross-reactivity. In the teratoma-derived tumors, absorption of the antiserum with testis or thymus eliminated or markedly reduced the PAP staining of primitive neuroepithelial cells, and only moderately reduced, but did not remove, that of neural cells in the mature neuropil. Among the proteins of neonatal mouse brain plasma membranes separated by polyacrylamide gel electrophoresis, there were six distinct bands indicating major proteins ranging from 26,000–54,000 daltons. Autoradiography of the antigen-antibody complexes with125I protein A on the same gels demonstrated three discrete bands of activity at 10,000–12,000, 76,000, and 97,000 daltons, and one greater than 130,000 daltons, suggesting that the immune serum recognizes only minor protein components of the mouse brain plasma membranes. The application of the PAP method to the recognition of neural cell surface antigens considerably enhances the potential of this antiserum as a tool for the early identification of primitive neural cells in the experimental mouse teratoma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00690638
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  • 2
    Electronic Resource
    Electronic Resource
    The kinetics of human glioblastomas maintained in an organ culture system (1983)
    Springer
    Acta neuropathologica 61 (1983), S. 1-9 
    Details
    ISSN: 1432-0533
    Keywords: Glioblastomas ; Organ culture method ; Autoradiography ; Kinetics ; Growth fraction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Five human glioblastomas maintained in an organ culture system were studied by autoradiography to determine, after 8 days in vitro, the growth fraction (GF) of the explants, their total cell cycle time (T C) and cell cycle phase durations (T S,T G1,T G2 andT M), and their potential doubling time (T pot) after pulse-labeling with [3H] TdR for 1 h. These parameters were derived from computer analysis of fraction of labeled mitoses (FLM) curves. The results fell into two groups. In two tumors, the cultures had a GF of 0.25 and 0.23. From the FLM curves were derived aT C of 89 and 83 h, aT S of 16.5 and 9.5 h, and aT G1 of 60 and 61 h.T M was estimated at 0.9 and 0.6 h, andT G2 12h. TheT pot was 12 days. These values approximate those reported for glioblastomas and other human malignancies in vivo. The explants of three other glioblastomas gave different FLM curves: the derivedT S were increased to 36 and 55 h, estimatedT M ranged from 2.4 to 4.5 h, andT G2 ranged from 11 to 20 h.T C andT G1 could not be estimated. In two tumors the GF was reduced to 0.12 and 0.11, with aT pot of respectively 52 and 39 days. These values are comparable to those reported for astrocytomas of intermediate malignancy. In the third tumor, the GF was only 0.014. The reduction in GF and the lengthening of cell cycle components in this group of explants are similar to the kinetic changes reported in some in vivo tumors and three-dimensional in vitro systems that have reached a plateau stage of growth. They are probably related to the greater opportunities for cell-to-cell contacts and the resulting increased differentiation favored by the organ culture technique.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00688379
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  • 3
    Electronic Resource
    Electronic Resource
    Qualitative and Quantitative Determination of Some Yellow, Orange and Red Food Dyes by Resonance Raman Spectroscopy (1983)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 48 (1983), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The use of resonance Raman spectroscopy in the determination of food dyes was studied with the yellow, orange and red dyes registrated by the European Economical Community. The natural dyes studied did not exhibit a resonance Raman spectrum, but most of the artificial dyes give spectra that can be used for analytical determinations. The detection and identification limits are sufficiently low for practical use. The identification capability and its advantage over absorption spectrometric measurements are demonstrated on a commercial bubble gum sample. Quantitative measurements indicated a good accuracy for this method.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1983.tb10780.x
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  • 4
    Electronic Resource
    Electronic Resource
    THE USE OF THE SYNTHETIC PEPTIDES γ-TYPE ENDORPHINS IN MENTALLY ILL PATIENTS (1982)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 398 (1982), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1982.tb39519.x
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  • 5
    Electronic Resource
    Electronic Resource
    The pathogenesis of lead encephalopathy (1980)
    Springer
    Virchows Archiv 387 (1980), S. 147-164 
    Details
    ISSN: 1432-2307
    Keywords: Lead encephalopathy ; Mitochondria ; Respiration ; Elemental microanalysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The toxic effects of inorganic lead feedings on the immature brain were studied in the rat pup. Beginning when litters were two weeks old, PbCO3 was fed to nursing mothers and then to pups directly after weaning. Results in lead-fed pups were compared to age-matched controls and to lead-fed young adult males (60 days old). Anaemia and growth failure developed in both pups and adults. In the second week, more than half the pups developed an encephalopathy, with haemorrhage and oedema predominately in the cerebellum and lead-containing densities in the cerebellar molecular layer. The latter were confirmed by X-ray microanalysis. No lead-fed adults showed signs of an encephalopathy. Cerebellar mitochondria from lead-fed pups, studied polarographically, showed a very early loss of respiratory control and a subsequent inhibition of phosphorylation-coupled respiration with NAD-linked substrates but not with succinate. Compared to the pup cerebellum, these changes were much less marked in immature cerebral mitochondria and were not found in adult cerebral or cerebellar mitochondria. Cerebral and cerebellar homogenates from immature and mature lead-fed animals showed large increases in lead content measured by atomic absorption spectrophotometry. Immature cerebellar mitochondrial lead contents were increased to the same extent as in the homogenates. Mitochondria from immature cerebrum and from both regions in the mature brain showed less immediate and smaller increases in lead content. In conclusion, altered mitochondrial respiration occurs early in regional and age-dependent association with lead encephalopathy in the rat pup. The development of lead encephalopathy also is associated with increased mitochondrial lead concentrations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00430696
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  • 6
    Electronic Resource
    Electronic Resource
    Hauser-Feshbach calculations in the presence of weakly absorbing channels with special reference to the elastic enhancement factor and the factorization assumption (1980)
    Springer
    The European physical journal 297 (1980), S. 153-160 
    Details
    ISSN: 1434-601X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract In an extension of previous work on the theory of statistical cross sections model calculations have been performed in the presence of weakly absorbing channels, a case which is of particular interest in applied work involving neutron capture or neutron scattering at low energies. Improved formulas for calculating the elastic enhancement factor and compound nucleus cross sections are presented. In our model calculations we find that the factorization condition for cross sections is not satisfied in cases where a few strongly and many weakly absorbing channels are mixed. However, even in these extreme cases, the formulas provide a good description of the computer-generated cross sections involving at least one strong channel. In addition, the structure of the formula for the elastic enhancement factor has been chosen so as to reproduce all known limiting values and to describe cases where many weakly absorbing channels contribute.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01421472
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  • 7
    Electronic Resource
    Electronic Resource
    Selective high metabolic lability of uridine triphosphate in response to glucosamine feeding of untransformed and polyoma virus-transformed hamster fibroblasts (1980)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 104 (1980), S. 253-259 
    Details
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Derepression of hexose transport in a line of Syrian hamster fibroblasts (Nil) and polyoma-transformed (PyNil) hamster fibroblasts is obtained when cells are either starved for glucose or fed with fructose as the only hexose source. D-glucosamine feeding of these cells does not alter the repressed state with regard to hexose transport. High, derepressed rates of galactose transport were changed to low, repressed rates, within 18 hours of refeeding glucose-starved cells with D-glucosamine as the only hexose source. Nil and PyNil cells, when cultured in the presence of D-glucosamine, undergo rapid reductions in total cellular uridine 5′-triphosphate (UTP) pool sizes. By contrast, the total cellular pools of adenosine 5′-triphosphate, guanosine 5′-triphosphate, and cytosine 5′-triphosphate (ATP, GTP, and CTP) were only moderately affected by the treatment of the cells with glucosamine. The metabolic drain of the UTP pools in PyNil cells was much more pronounced than in the untransformed cells. The larger and more rapid metabolic lability of UTP pools in the transformed cells may be the primary reason for the selective toxicity of glucosamine on tumor cells. A comparison of the effects of glucosamine on hexose-starved Nil and PyNil cells demonstrated that only the untransformed cells were able to utilize glucosamine to increase the hexose starvation-depleted pools of all nucleoside triphosphates. Accumulation of UDP-glucosamine and UDP-N-acetylglucosamine followed the reduction in the UTP pools. Inhibition of protein synthesis by cycloheximide during glucosamine feeding led to higher levels of UDP-glucosamine and UDP-N-acetylglucosamine accumulation. It is suggested that the drain of UTP pools during glucosamine treatment proceeds through the formation of the UDP-aminosugars which turn over due to the action of intracellular UDP-aminosugar pyrophosphatase activities.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jcp.1041040216
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