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  • 1
    Electronic Resource
    Electronic Resource
    Improved growth conditions for hyphomicrobium sp. B-522 and two additional strains (1982)
    Springer
    Archives of microbiology 131 (1982), S. 32-35 
    Details
    ISSN: 1432-072X
    Keywords: Hyphomicrobium ; Growth requirements ; Growth improvement ; Vitamin B12 ; C-1 utilization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Growth yields and rates of 3 hyphomicrobia were improved by varying components of or adding compounds to medium 337. Methanol (0.5% v/v), and similarly methylamine·HCl (3.38g/l), were optimal among 22 C-sources tested; increasing the methylamine·HCl concentration to 5.07g/l gave higher Hyphomicrobium B-522 yields but also prolonged lag periods. Ten C-sources (organic acids, alcohols) stimulated growth slightly but significantly, even in subcultures. Sugar compounds were not utilized. Strains B-522 and ZV-580 were stimulated by l-lysine and gluconate, while NQ-521 gr was stimulated by aspartate. N-Sources tested were inorganic (3), organic (3), or complex (3). (NH4)2SO4 (0.5g/l) was optimal for strains ZV-580 and NQ-521 gr, but Hyphomicrobium B-522 grew best with urea-N. With NH 4 + , strain B-522 grew as homogeneous suspension, all other N-sources caused clumping and pellicle formation. Inorganic requirements (PO 4 3- , Mg, Ca, Fe, Mn, Mo) of strains B-522 and ZV-580 were optimized. Addition of Ni, Co, or Zn had no effect; metals “44” or Cu, resulted in growth inhibition. Vitamin B12 stimulated Hyphomicrobium B-522; 2.5μg/l B12 decreased the doubling time from 9.3–10.8h to 5.4–5.8h. All combined single improvements resulted in a protein increase of 557% (B-522), 141% (NQ-521 gr), or 109% (ZV-580), respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00451495
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  • 2
    Electronic Resource
    Electronic Resource
    Continuous culture and synchronization ofHyphomicrobium sp. B-522 (1982)
    Springer
    Archives of microbiology 132 (1982), S. 96-99 
    Details
    ISSN: 1432-072X
    Keywords: Hyphomicrobium ; Continuous culture ; Synchronization technique ; Synchronous swarmer cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A technique was developed for synchronization ofHyphomicrobium sp. strain B-522. Bacteria were grown in continuous culture with methanol (0.1%; v/v) growth limiting. Vitamin B12 (2.5 μg/l) was necessary to obtain steady state growth. The critical dilution rate wasD c =0.112; maximum cell output occurred atD=0.105 (Dx=30 mg l-1 h-1). Continuous cultures ofHyphomicrobium B-522 atD=0.110 were used to obtain cells for synchronization experiments. Synchronization was achieved by trapping young hyphal and budding cells in a glass wool column, while the initial swarmer cells were allowed to pass through. By semicontinuously rinsing the system, newly produced swarmers could be sampled in the effluent. The mean length of these synchronous swarmer cells was 1.25 μm (s=±0.13 μm; range 0,6 μm) as compared to 1.40 μm (s=±0.21 μm; range 1.2 μm) for swarmer cells of the continuous culture. Division of synchronous swarmer populations was completed after 7 h; the synchronization index was 0.76.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00690826
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  • 3
    Electronic Resource
    Electronic Resource
    Cell wall studies on budding bacteria of the Planctomyces/Pasteuria group and on a Prosthecomicrobium sp. (1984)
    Springer
    Archives of microbiology 138 (1984), S. 200-205 
    Details
    ISSN: 1432-072X
    Keywords: Budding bacteria ; Cell wall composition ; Antibiotic resistance ; Murein ; Taxonomy of eubacteria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Seven strains of budding, non-prosthecate bacteria belonging to the Planctomyces/Pasteuria group and a Prosthecomicrobium sp. were examined for muramic and diaminopimelic acids. These typical components of Gram-negative murein were found only in Prosthecomicrobium strain IFAM 1314, but they could not be detected in seven budding bacteria. Electron micrographs of ultrathin cell wall sections of strains IFAM 1313 and 1317 showed a membrane with bilayer structure outside the cytoplasmic membrane. 10% sodium dodecylsulfate treatment (30 min, 100°C) allowed the isolation of highly stable cell sacculi which, upon chemical analysis, proved to be mainly proteinaceous. The budding bacteria also showed considerable resistance against penicillin G, ampicillin, cephalotin and D-cycloserin. Our data indicate that these bacteria lack an ordinary Gram-negative type of murein and, instead, carry a stable protein envelope.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00402120
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  • 4
    Electronic Resource
    Electronic Resource
    Electron irradiation induced vitrification at dislocations in quartz (1980)
    [s.l.] : Nature Publishing Group
    Nature 287 (1980), S. 314-316 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Quartz, the second most abundant crustal mineral, is important in the study of deformation within the Earth's crust. Plastically deformed quartz has therefore been the subject of numerous studies3'5 from which two significant findings have emerged: (1) quartz deforms by dislocation creep mechanisms ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/287314a0
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  • 5
    Electronic Resource
    Electronic Resource
    A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid (1983)
    [s.l.] : Nature Publishing Group
    Nature 303 (1983), S. 179-180 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Fig. 1 a, The construction of pALlOSO. The part of the T-DNA of pTiAchS present on pOTYS is shown in the bottom right-hand corner. pOTYS was constructed from subclones of BamHl fragment 8 and Hindlll fragment 1, using pJDB 207 as a vector plasmid. For pALlOSO, R772 and pOTYS, the restriction sites ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/303179a0
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  • 6
    Electronic Resource
    Electronic Resource
    Anti-phase domain boundary tubes in Ni 3 Al (1982)
    [s.l.] : Nature Publishing Group
    Nature 300 (1982), S. 621-623 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] In many nickel-based alloys the high-temperature strength is provided by the y' precipitate based on Ni3Al, an ordered phase with the L12 structure. The flow stress of the y' phase reaches a peak value at about 700 C. Several theories have been put forward to explain this anomalous behaviour3 with ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/300621a0
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  • 7
    Electronic Resource
    Electronic Resource
    Isolation of symbiotically defective mutants in Rhizobium leguminosarum by insertion of the transposon Tn5 into a transmissible plasmid (1980)
    Springer
    Molecular genetics and genomics 178 (1980), S. 185-190 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Selection was made for the transposition of the kanamycin resistance transposon Tn5 from a location on the chromosome of R. leguminosarum into a transmissible, bacteriocinogenic plasmid that also carries genes required for the induction of nitrogen-fixing nodules on peas. One hundred and sixty independent insertions into transmissible plasmids were isolated. When these plasmids were transferred by conjugation into a non-nodulating strain, which carries a deletion in one of its resident plasmids, of the 160 isolates tested 14 yielded transconjugants that formed nodules that did not fix nitrogen (Fix-) and in a further 15 cases the transconjugants were unable to form nodules (were Nod-). When transferred to a symbiotically proficient strain (i.e. Nod+ Fix+) none of the transconjugants was symbiotically defective; thus the mutations were not dominant. When kan was transduced from the clones that generated Fix- transconjugants into a Fix+ recipient the majority of transductants inherited Fix- indicating that the insertion of Tn5 had induced the symbiotic mutations. Transduction of kan, from the clones that failed to donate Nod+ by conjugation to strain 6015, occurred at barely detectable frequencies and it was not possible to demonstrate transduction of Nod-. kan was co-transduced with Nod+ from some of the clones and some of these transductants also inherited the ability to produce medium bacteriocin and to transfer at high frequency by conjugation. Thus the genes for all these characters are closely linked.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00267228
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  • 8
    Electronic Resource
    Electronic Resource
    Agrobacterium tumefaciens T-DNA in the yeast Saccharomyces cerevisiae (1984)
    Springer
    Molecular genetics and genomics 195 (1984), S. 209-214 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A section of the octopine Ti plasmid pTiACH5 including the region always integrated in crown gall tumour cells (the TL-DNA) was cloned into the yeast — E. coli shuttle vector pJDB207. This plasmid, pOTY8, was transformed into S. cerevisiae where transcription of the T-DNA was demonstrated, but there was no evidence for the production of octopine synthetase. The TL-DNA was cloned into a derivative of pJDB207 unable to replicate in yeast, but the TL-DNA did not appear to contain any autonomously replicating sequences (ars) functional in yeast.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00332748
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