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  • 1
    Electronic Resource
    Electronic Resource
    A nystatin-resistant mutant of Rhodotorula gracilis. Transport properties and sterol content (1982)
    Springer
    Archives of microbiology 132 (1982), S. 313-316 
    Details
    ISSN: 1432-072X
    Keywords: Rhodotorula gracilis ; Nystatin resistance ; Sterol content ; Plasma membrane permeability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A nystatin-resistant mutant of Rhodotorula gracilis was obtained by treatment of the wild strain cells with N-methyl-N-nitro-N-nitrosoguanidine and selected on agar plates containing 150 μg nystatin/ml. Three important transport functions of the plasma membrane of mutant cells: the accumulation of monosaccharides, the generation and maintenance of the pH-gradient and of the membrane potential, as well as the cell respiration were insensitive to at least 10-5 M nystatin. This concentration of nystatin inhibited completely all these processes in wild strain cells. Analysis of cellular sterols revealed a defect of ergosterol biosynthesis in the mutant, which was localized at the last oxidative step between 5,6-dihydroergosterol and ergosterol.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00413381
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Changes in myocardial substrate utilisation and protection of ischemic stressed myocardium by oxfenicine [(S)-4-hydroxyphenylglycine] (1984)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 327 (1984), S. 70-74 
    Details
    ISSN: 1432-1912
    Keywords: Cardiac metabolism ; Myocardial ischemia ; Oxfenicine ; Oxygen debt ; Oxygen repayment ; Release of electrolytes and substrates
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Potential protective effects of oxfenicine [(S)-4-hydroxyphenylglycine] in ischemic stressed canine myocardium have been studied. This compound is characterized as a drug leading to metabolic inhibition of free fatty acid (FFA) metabolism. The drug (0.06 mmol·kg−1 body weight) caused no changes in hemodynamics or energy demand (Et) but depressed myocardial oxygen consumption (MVO2) by 11% (P〈0.02). Significant changes in FFA and lactate metabolism were observed: lactate extraction (EX) increased from 22.5–37.1 μmol/min, extraction ratio (EXR) from 16.5–30% and oxygen extraction ratio (OER) from 24.8–38%; EX of FFA decreased from 6900–5000 nmol/min, EXR from 48.2–31.4% and OER from 59.7–46.6%. Arterial concentrations of FFA and lactate remained unchanged. EX, EXR and OER of glucose were not affected under basic conditions. In the same collective, repeated ischemia (3 min) was produced by proximal occlusion of the left anterior descending artery (LAD). The efficiency of the drug was examined by (a) the amounts of ischemia metabolites released in the early reperfusion and (b) quantification of O2-debt and O2-repayment in the occlusion- and reperfusion periods. Compared to control occlusions, premedication led to a reduced O2-debt (P〈0.01) combined with a reduced amount of oxygen additionally taken up in the early reperfusion (P〈0.05). Furthermore, release of potassium increased (+7.1%; P〈0.05); release of lactate (-32%, P〈0.001) and inorganic phosphate (-34%, P〈0.01) decreased. These data give support to the concept that a pharmacologically induced shift of cardiac metabolism with reduction of FFA utilisation may be favourable in circumstances with limited oxygen supply.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00504994
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Esthesioneuroblastoma: Ultrastructural, immunohistological and biochemical investigation of one case (1984)
    Springer
    European archives of oto-rhino-laryngology and head & neck 239 (1984), S. 133-144 
    Details
    ISSN: 1434-4726
    Keywords: Esthesioneuroblastoma ; Intermediate filaments ; Secretory granules ; Ultrastructure ; Immunohistology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A case of esthesioneuroblastoma, the pathological diagnosis of which almost always causes great difficulties, was investigated ultrastructurally, biochemically, and immunohistologically, using antibodies against the five known types of intermediate filaments [keratin, vimentin, desmin, glial fibrillary acidic protein (GFAP) and neurofilaments]. The tumour cells did not react with antibodies against any of the five intermediate filament proteins. Ultrastructural investigations showed dense cored secretory granules in the cytoplasm and cell processes. Thus, immunohistology offers by “exclusion” a differential diagnosis to avoid often misdiagnosed tumours (undifferentiated carcinomas, embryonal rhabdomyosarcomas, and malignant lymphomas), since carcinomas react with antikeratin, embryonal rhabdomyosarcomas with antibodies to desmin and malignant lymphomas show immunofluorescence with antibodies to vimentin. The biological behaviour (age distribution, tendency to metastasize), the normal values of biochemical parameters, homovanillic acid and vanilmandelic acid (HVA, VMA), and the absence of neurofilaments distinguish this type of tumour from the peripheral sympathetic neuroblastoma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00463554
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    Paper (German National Licenses)
    Fulltext
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