ISSN:
1432-072X
Keywords:
Rhodotorula gracilis
;
Nystatin resistance
;
Sterol content
;
Plasma membrane permeability
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract A nystatin-resistant mutant of Rhodotorula gracilis was obtained by treatment of the wild strain cells with N-methyl-N-nitro-N-nitrosoguanidine and selected on agar plates containing 150 μg nystatin/ml. Three important transport functions of the plasma membrane of mutant cells: the accumulation of monosaccharides, the generation and maintenance of the pH-gradient and of the membrane potential, as well as the cell respiration were insensitive to at least 10-5 M nystatin. This concentration of nystatin inhibited completely all these processes in wild strain cells. Analysis of cellular sterols revealed a defect of ergosterol biosynthesis in the mutant, which was localized at the last oxidative step between 5,6-dihydroergosterol and ergosterol.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00413381
