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  • 1980-1984  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Gd(-) Gifu and Gd(-) Fukuoka. Two new variants of glucose-6-phosphate dehydrogenase found in Japan (1984)
    Springer
    Human genetics 〈Berlin〉 66 (1984), S. 276-278 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Two new glucose-6-phosphate dehydrogenase (G6PD) variants were discovered in Japan. The first, found in a 9-year-old male, was associated with chronic hemolysis and hemolytic crises after upper respiratory infections. The enzyme activity of the variant was 2.9% of normal. The patient's G6PD showed an increased utilization of substrate analogue, deamino-NADP, and thermal instability. The second variant occurred in a 7-year-old male with druginduced hemolysis. The main enzymatic characteristics were reduced enzyme activity, being 6.4% of normal, faster-thannormal anodal electrophoretic mobility, slightly high Michaelis constant for glucose-6-phosphate, thermal instability, and biphasic pH optima. Enzymatic properties of these variants allowed each to be distinguished from previously reported variants. The first variant was designated Gd (-) Gifu and the other, Gd (-) Fukuoka.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00286616
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Human erythroid cell lines derived from a patient with acute erythremia (1981)
    Springer
    Journal of cancer research and clinical oncology 102 (1981), S. 49-55 
    Details
    ISSN: 1432-1335
    Keywords: KMOE cells ; Erythroid cells ; Human cell line ; Erythroid differentiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Three continuous human cell lines, designated KMOE, derived from a patient with acute erythremia (Di Guglielmo's disease) are reported. The cell lines are the cultures of (1) bone marrow cells, (2) peripheral blood cells, and (3) cells from a tumor developed into an athymic nude mouse after transplantation of the cultured bone marrow cells. Cells of all three lines show morphology of immature erythroblast and have i(17q) marker chromosome. They are negative for both Philadelphia chromosome and Epstein-Barr virus nuclear antigen. Although all KMOE cells in suspension culture are benzidine-negative, benzidine-positive cells are found within colonies formed in semi-solid culture media. The relative number of colonies with benzidine-positive cells is increased when sodium butyrate is added to the culture. The KMOE cell lines are human erythroid cell lines with erythroblastic morphology and still retain their tendency for differentiation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00410534
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    Paper (German National Licenses)
    Fulltext
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