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  • 1
    Electronic Resource
    Electronic Resource
    Transport by reconstituted lactose carrier from parental and mutant strains ofEscherichia coli (1984)
    Springer
    The journal of membrane biology 79 (1984), S. 185-193 
    Details
    ISSN: 1432-1424
    Keywords: reconstitution ; lactose transport ; membrane potential ; pH gradient ; proteolytic enzymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary The lactose transport carrier from parental (X71/F'W3747) and mutant cells (54/F'5441) was reconstituted into proteoliposomes. Transport by the counterflow assay showed slightly greater activity in proteoliposomes prepared from extracts of the mutant membranes compared with that for the parental cell. The mutant carrier showed a threefold lowerK m but similarV max compared to the parent. On the other hand proteoliposomes from the mutant showed a defect in protonmotive force-driven accumulation, compared with the parent. With a pH gradient (inside alkaline) plus a membrane potential (inside negative) the parental proteoliposomes accumulated lactose 25-fold over the medium concentration while the mutant proteoliposomes accumulated sixfold. In a series of experiments proteoliposomes were exposed to proteolytic enzymes. Chrymotrypsin treatment resulted in 30% inhibition of counterflow activity for the reconstituted carrier from both parent and mutant. Papain produced 84% inhibition of transport by the reconstituted parental carrier but only 41% of that of the mutant. Trypsin and carboxypeptidase Y treatment had no effect on counterflow activity of either parent or mutant. Exposure of purified lactose carrier in proteoliposomes to carboxypeptidase Y resulted in the release of alanine and valine, the two C-terminal amino acids predicted from the DNA sequence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01872122
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  • 2
    Electronic Resource
    Electronic Resource
    Membrane transport ofp-nitrophenyl-α-galactoside by the melibiose carrier ofEscherichia coli (1980)
    Springer
    The journal of membrane biology 56 (1980), S. 169-175 
    Details
    ISSN: 1432-1424
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary p-Nitrophenyl-α-galactoside (α-pNPG) was found to be a substrate for the melibiose transport system ofEscherichia coli. This sugar enters induced cells via the carrier and is split by α-galactosidase to galactose andp-nitrophenol. In mutant cells lacking the α-galactosidase [3H]-α-pNPG accumulated to concentrations 15 times higher than the external medium. The transport of α-pNPG is inhibited by both Na+ and Li+. Na+ (10mm) reduced the Km for α-pNPG from 0.45 to 0.18mm and reduced theV max from 6.7 nmoles/min/mg dry wt to a value of 3.0.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01875968
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  • 3
    Electronic Resource
    Electronic Resource
    Reaction of Sulfur Dioxide and Hydrogen Sulfide with Porous Calcined Limestone (1980)
    s.l. : American Chemical Society
    Industrial and engineering chemistry 19 (1980), S. 565-572 
    Details
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/i260076a011
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  • 4
    Electronic Resource
    Electronic Resource
    THE CATION SPECIFICITY FOR THE MELIBIOSE TRANSPORT SYSTEM OF E. COLI (1980)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 341 (1980), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1980.tb47191.x
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  • 5
    Electronic Resource
    Electronic Resource
    A sampling artefact affecting the uranium content of deep-sea porewaters obtained from cores (1984)
    [s.l.] : Nature Publishing Group
    Nature 308 (1984), S. 263-266 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The porewaters were collected on RRS Discovery Cruise 129 in 1982, using the Institute of Oceanographie Sciences (IOS) Mark II in situ porewater sampler12, a box corer13 to retrieve short sediment sections with undisturbed interfaces, and a Kastenlot corer to recover longer (2 m) sections. Subcores ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/308263a0
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  • 6
    Electronic Resource
    Electronic Resource
    Inhibition of active bone resorption by copper (1981)
    Springer
    Calcified tissue international 33 (1981), S. 35-39 
    Details
    ISSN: 1432-0827
    Keywords: Copper ; Resorption ; Bone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary An investigation of the role of copper in bone metabolism was undertaken. Explanted calvaria from 6-day-old mice were grown for 48 h in medium with and without the addition of copper sulfate. Active resorption was found to be significantly inhibited in the presence of copper sulfate concentrations of 10−6M and above. Copper sulfate concentrations of 10−5M and above inhibited hydroxyproline, protein, and DNA synthesis. Lower concentrations were ineffective. The effect of 5 × 10−6M copper sulfate on resorption was reversible. Several other compounds were tested for similar effects and at 5 × 10−6M were found to inhibit bone resorption in the order: copper sulfate 〉 brown gold chloride 〉 sodium aurothiomalate 〉 zinc sulfate 〉 sodium sulfate. The copper sulfate effect was twice that of sodium aurothiomalate, and sodium sulfate was not significantly inhibitory. The results suggest that the high serum copper levels associated with rheumatoid arthritis may reflect the activity of a hypothetical control mechanism of bone resorption. In the diseased state this would act to restore the normal rate of bone resorption.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02409410
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  • 7
    Electronic Resource
    Electronic Resource
    Imaging properties and applications of scanning optical microscopes (1980)
    Springer
    Applied physics 22 (1980), S. 119-128 
    Details
    ISSN: 1432-0630
    Keywords: 42.30 ; 42.80 ; 72.40
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract This review paper is concerned with the imaging properties and major uses of scanning optical microscopes. It is shown that the confocal scanning microscope exhibits a form of super-resolution and that the instrument in general has great application in nonlinear microscopy and the inspection of electronic devices.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00885994
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  • 8
    Electronic Resource
    Electronic Resource
    Differential amplitude contrast imaging in the scanning optical microscope (1983)
    Springer
    Applied physics 32 (1983), S. 187-191 
    Details
    ISSN: 1432-0649
    Keywords: 42.80
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A split detector is used in a scanning optical microscope to produce high-quality differential amplitude contrast images. A slight lateral offset in the detector position is shown to introduce information about object height variations to the image. These results are compared with images obtained by electrical differentiation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00688286
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  • 9
    Electronic Resource
    Electronic Resource
    Three-dimensional surface measurement using the confocal scanning microscope (1982)
    Springer
    Applied physics 27 (1982), S. 211-213 
    Details
    ISSN: 1432-0649
    Keywords: 06 ; 42.80
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The use of the depth discriminination property of the confocal scanning microscope for surface profiling has been adapted to provide a method of high-resolution three-dimensional surface profilometry. Measurements on a semiconductor specimen demonstrate the technique; depth variations of the order of 0.1 μm are clearly resolved.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00697444
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  • 10
    Electronic Resource
    Electronic Resource
    Optical fibre profiling using a scanning optical microscope (1980)
    Springer
    Optical and quantum electronics 12 (1980), S. 341-345 
    Details
    ISSN: 1572-817X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Physics
    Notes: Abstract A scanning optical microscope is used to measure directly the refractive-index profile of an optical fibre. The effects of illuminating the fibre end with a highly convergent beam of light are considered.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00620289
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