ISSN:
1432-1912
Keywords:
Tetanus
;
Botulism
;
Acetylcholine
;
Nerve tissue
;
Cell cultures
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Primary nerve cell cultures derived from embryonic rat central nervous system form [3H]ACh from exogenous [3H]Ch, and release it upon potassium depolarization. Pretreatment of the cultures with botulinum-A toxin or tetanus toxin diminishes the cellular accumulation of [3H]ACh. Poisoning the cultures during the period of [3H]Ch uptake fails to lower [3H]ACh formation. Dependent on dosage, both toxins suppress the release of [3H]ACh upon potassium depolarization. Heat-denaturated toxins as well as tetanus toxin preincubated with tetanus antitoxin were without effect.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00508058
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