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  • 1975-1979  (6)
  • 1
    Electronic Resource
    Electronic Resource
    Improved methodology for ATP determination in marine environments (1976)
    Springer
    Marine biology 34 (1976), S. 143-149 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A simple technique is described for de-salting and concentrating adenosine triphosphate (ATP) extracted from seawater or marine sediment samples prior to assays with the standard luciferin-luciferase procedure. The technique involves chromatography of H2SO4 extracts on columns of activated carbon. The efficiency of ATP recovery from marine sediments using this pre-treatment was superior to that attained with either boiling “Tris” extraction or with H2SO4 extraction without subsequent purification. All ions which interfere with the luciferase reaction or precipitate ATP upon neutralization of acid extracts are removed with this procedure, thus eliminating the 50- to 100-fold dilutions required with other acid-extraction procedures. In addition, the purified ATP extracts may be concentrated up to 100-fold, thus greatly improving the sensitivity of ATP measurement in samples containing very low biomass. This procedure has been applied to ATP determination in marine sediments in the upwelling area off northwest Africa. The average ATP concentrations in the upper 6 cm of sediment from 1000 and 200 m were 195 and 545 ng per ml wet sediment, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00390756
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Some experiments on phosphate assimilation by coastal marine plankton (1977)
    Springer
    Marine biology 40 (1977), S. 9-18 
    Details
    ISSN: 1432-1793
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A study of phosphate assimilation by coastal marine plankton revealed that both phytoplankton and microheterotrophs incorporated radioactive phosphorus (33P). Size fractionation of the particulate matter (using 1 μm pore diameter Nucleopore® membrane filters), antibiotic treatment (using garamycin), and independent estimaties of photoautotrophic (14CO2 uptake) and heterotrophic (3H-glucose uptake) activities were employed to separate phyto- and bacterioplankton phosphate uptake. Results indicated that phytoplankton 33P-uptake was best estimated by the fraction of particulate matter retained on the 1 μm membrane filters. Usually, less than 10% of the phytoplankton (based on chlorophyll a measurements) passed the 1 μm pore-diameter filters, whereas about 90% of the heterotrophic activity passed. At least 50% of the 33P-uptake was associated with the 〈1 μm fraction. It may be possible to resolve the phytoplankton and bacterial contributions to 33P-uptake by comparing the percent of total 33P-uptake with the percent of total 3H-glucose uptake associated with the 〉1 μm fraction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00390622
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Radioisotope choroid plexography. A preliminary report (1976)
    Springer
    Neuroradiology 11 (1976), S. 249-253 
    Details
    ISSN: 1432-1920
    Keywords: Radioisotope plexography ; Choroid plexus ; Hydrocephalus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Radioisotope plexography seems as easy and innocuous as routine brain scanning. An introductory method is given which could be improved. The choroid plexuses of the four ventricles were well visualized in 15 patients. Consideration is given to the possible labeling process. Besides its morphological value, the test could give interesting clues in the dynamic study of hydrocephalus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00328381
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    CAT of the spine and spinal cord (1978)
    Springer
    Neuroradiology 16 (1978), S. 369-370 
    Details
    ISSN: 1432-1920
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary CAT for spine and spinal cord studies was carried out in 182 patients aged 7–80 years during 12 months (April 1977 to March 1978) with the EMI body scanner CT 5005. Three methods were used: (1) standard (noncontrast) scanning; (2) the same, with IV contrast enhancement; (3) the same again, after CSF enhancement with Amipaque (computer-assisted myelography). The combined use of Amipaque and CT seems particularly interesting.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00395307
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Dissolved ATP in the sea and its utilisation by marine bacteria (1977)
    [s.l.] : Nature Publishing Group
    Nature 267 (1977), S. 696-698 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Although the particulate ATP content of marine seston (suspended particles) has been used extensively as a sensitive indicator of microbial biomass in oceans and lakes4, the presence of DATP in seawater had not been demonstrated conclusively9. This has been due, in part, to the absence of a ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/267696a0
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Effect of germanic acid on developing cell walls of diatoms (1977)
    Springer
    Protoplasma 93 (1977), S. 191-204 
    Details
    ISSN: 1615-6102
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effects of 0.1 and 1.0 germanic acid/silicic acid concentrations on wall development inNitzschia alba, Navicula pelliculosa, Cylindrotheca fusiformis, and Cyclotella nana are described. Valve development and differentiation are interrupted, and sometimes completely blocked. Girdle band formation is also affected.N. alba produces multiple walls (at 0.1 [Ge]/[Si]) and dense cytoplasmic granules (at 1.0 [Ge]/[Si]).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01275653
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    Paper (German National Licenses)
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