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Improved methodology for ATP determination in marine environments (1976)

Hodson, R. E. ; Holm-Hansen, O. ; Azam, F.

Springer

Marine biology 34 (1976), S. 143-149

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ISSN: 1432-1793

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract A simple technique is described for de-salting and concentrating adenosine triphosphate (ATP) extracted from seawater or marine sediment samples prior to assays with the standard luciferin-luciferase procedure. The technique involves chromatography of H2SO4 extracts on columns of activated carbon. The efficiency of ATP recovery from marine sediments using this pre-treatment was superior to that attained with either boiling “Tris” extraction or with H2SO4 extraction without subsequent purification. All ions which interfere with the luciferase reaction or precipitate ATP upon neutralization of acid extracts are removed with this procedure, thus eliminating the 50- to 100-fold dilutions required with other acid-extraction procedures. In addition, the purified ATP extracts may be concentrated up to 100-fold, thus greatly improving the sensitivity of ATP measurement in samples containing very low biomass. This procedure has been applied to ATP determination in marine sediments in the upwelling area off northwest Africa. The average ATP concentrations in the upper 6 cm of sediment from 1000 and 200 m were 195 and 545 ng per ml wet sediment, respectively.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00390756

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Dissolved ATP in the sea and its utilisation by marine bacteria (1977)

AZAM, F. ; HODSON, R. E.

[s.l.] : Nature Publishing Group

Nature 267 (1977), S. 696-698

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ISSN: 1476-4687

Source: Nature Archives 1869 - 2009

Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics

Notes: [Auszug] Although the particulate ATP content of marine seston (suspended particles) has been used extensively as a sensitive indicator of microbial biomass in oceans and lakes4, the presence of DATP in seawater had not been demonstrated conclusively9. This has been due, in part, to the absence of a ...