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  • 1
    Electronic Resource
    Electronic Resource
    Some properties of vacuoles isolated from Neurospora crassa slime variant (1979)
    Springer
    Archives of microbiology 120 (1979), S. 31-34 
    Details
    ISSN: 1432-072X
    Keywords: Neurospora crassa ; Slime variant ; Vacuoles ; Isolation ; Hydrolases ; Arginine permease
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A method for the isolation of vacuoles based on polybase induced lysis of protoplasts of the cell wall deficient Neurospora crassa slime variant is described. Isolated vacuoles are characterized by 12 to 50 times increased specific activities of several hydrolases as compared with the total homogenate of protoplasts. Total α-amino nitrogen, arginine, and polyphosphate are also greatly enriched in these vacuoles. Vacuoles are equipped with a permease for the transport of basic amino acids across the tonoplast.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00413268
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Subcellular distribution of yeast invertase isoenzymes (1975)
    Springer
    Archives of microbiology 103 (1975), S. 51-55 
    Details
    ISSN: 1432-072X
    Keywords: Yeast ; Invertase ; Isoenzymes ; Localization ; Vacuoles ; Spheroplasts ; Lipid Granules ; Cytosol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Homogenates from yeast cells contain 1% or less of sedimentable invertase activity. Sedimentability is equally low in homogenates from cells repressed or derepressed with regard to invertase secretion. Intracellularly, the mannanprotein form of invertase is largely localized in vacuoles whereas the small isoenzyme is largely present in the soluble cell fraction. These findings indicate that vesicles are not involved in the secretion of invertase. A soluble mode of invertase secretion is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00436329
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Zellkompartimentierung am Beispiel der pflanzlichen Vakuole (1979)
    Springer
    Naturwissenschaften 66 (1979), S. 343-346 
    Details
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Notes: Abstract The significance of membrane-encircled subcellular compartments with regard to requirements of metabolism is shown in the case of the central vacuole of plant cells. This compartment allows the synthesis and storage of secondary products of metabolism which are potentially toxic for the plant. Since unspecific hydrolases are also located in this compartment, it is most probably involved in cellular lytic processes. Moreover, the central vacuoles have important functions in the temporary removal of primary metabolites from the cytoplasm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00368468
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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