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  • 1
    Electronic Resource
    Electronic Resource
    Some properties of vacuoles isolated from Neurospora crassa slime variant (1979)
    Springer
    Archives of microbiology 120 (1979), S. 31-34 
    Details
    ISSN: 1432-072X
    Keywords: Neurospora crassa ; Slime variant ; Vacuoles ; Isolation ; Hydrolases ; Arginine permease
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A method for the isolation of vacuoles based on polybase induced lysis of protoplasts of the cell wall deficient Neurospora crassa slime variant is described. Isolated vacuoles are characterized by 12 to 50 times increased specific activities of several hydrolases as compared with the total homogenate of protoplasts. Total α-amino nitrogen, arginine, and polyphosphate are also greatly enriched in these vacuoles. Vacuoles are equipped with a permease for the transport of basic amino acids across the tonoplast.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00413268
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  • 2
    Electronic Resource
    Electronic Resource
    Subcellular distribution of yeast invertase isoenzymes (1975)
    Springer
    Archives of microbiology 103 (1975), S. 51-55 
    Details
    ISSN: 1432-072X
    Keywords: Yeast ; Invertase ; Isoenzymes ; Localization ; Vacuoles ; Spheroplasts ; Lipid Granules ; Cytosol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Homogenates from yeast cells contain 1% or less of sedimentable invertase activity. Sedimentability is equally low in homogenates from cells repressed or derepressed with regard to invertase secretion. Intracellularly, the mannanprotein form of invertase is largely localized in vacuoles whereas the small isoenzyme is largely present in the soluble cell fraction. These findings indicate that vesicles are not involved in the secretion of invertase. A soluble mode of invertase secretion is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00436329
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  • 3
    Electronic Resource
    Electronic Resource
    Isolation and properties of the plasmalemma in yeast (1967)
    Springer
    Archives of microbiology 58 (1967), S. 201-211 
    Details
    ISSN: 1432-072X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A method is described for the isolation of fragments of the plasmalemma based on differential and density gradient centrifugation using cell free extracts from anaerobically grown Saccharomyces cerevisiae. Electron microscopically investigated frozen-etched specimens of isolated plasmalemma revealed the presence of globular particles attached to the outer surface of the membrane; these particles correspond to those observed in situ. In isolated plasmalemma a high specific activity of Mg++-dependent ATPase, which is not sensitive to Oligomycin, is present. Yeast plasmalemma contains protein, lipids (including phospholipids) and an appreciable amount of polysaccharide. Hydrolysis of this polysacharide yields only mannose. The treatment of the isolated plasmalemma with detergents liberates the globular particles which can be isolated by density gradient centrifugation. Protein and polysaccharide occur in the respective fraction; therefore the globular particle represents a mannan-protein. It is concluded that the particles, which cover the plasma-membrane of plant cells, represent glycoproteins, that is, building stones to be incorporated into the fibrillar network of the cell walls.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00408804
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  • 4
    Electronic Resource
    Electronic Resource
    Lichthemmung der Oxydation von Krebscyclus-Säuren durch Blumenkohl-Mitochondrien (1962)
    Springer
    Cellular and molecular life sciences 18 (1962), S. 133-134 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The oxidation of succinic-, malic-, and α-ketoglutaric acid by cauliflower mitochondria is slowed down by strong visible light. The cytochrome-c-oxidase activity is also inhibited by light and the pigment is completely reduced in time. It is concluded that the Krebscycle is inhibited by light.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02153858
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  • 5
    Electronic Resource
    Electronic Resource
    Cell wall degradation in senescing tobacco leaf discs (1974)
    Springer
    Cellular and molecular life sciences 30 (1974), S. 98-99 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Zusammenfassung In ausgestanzten Blattstücken vonNicotiana tabacum, welche auf Wasser schwimmend inkubiert werden, findet im Verlauf von 7 Tagen ein beträchtlicher Abbau der Hemizellulose statt. Benzyladenin (10−5 M) verzögert den Abbau, Abscisinsäure (5×10−5 M) verstärkt ihn. Der Gehalt an Zellulose verändert sich im Verlauf der Alterung kaum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01921624
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  • 6
    Electronic Resource
    Electronic Resource
    Zellkompartimentierung am Beispiel der pflanzlichen Vakuole (1979)
    Springer
    Naturwissenschaften 66 (1979), S. 343-346 
    Details
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Notes: Abstract The significance of membrane-encircled subcellular compartments with regard to requirements of metabolism is shown in the case of the central vacuole of plant cells. This compartment allows the synthesis and storage of secondary products of metabolism which are potentially toxic for the plant. Since unspecific hydrolases are also located in this compartment, it is most probably involved in cellular lytic processes. Moreover, the central vacuoles have important functions in the temporary removal of primary metabolites from the cytoplasm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00368468
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  • 7
    Electronic Resource
    Electronic Resource
    A lysosomal aminopeptidase isozyme in differentiating yeast cells and protoplasts (1971)
    Springer
    Planta 96 (1971), S. 43-53 
    Details
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Cells of Saccharomyces cerevisiae that have been growing exponentially for many generations contain low activities of lysosomal enzymes. In contrast to such fully adapted cells, differentiating or resting cells contain comparatively high activities of these enzymes. Thus, the digestive enzymes seem to be involved in the process of biochemical differentiation. One of the four aminopeptidase isozymes present in extracts from yeast cells is localized in the vacuoles. This lysosomal enzyme can be separated from the other aminopeptidases by Sephadex G-150 gel filtration. Its specific activity is about 4 times higher in stationary cells than in exponentially growing cells. Upon incubating protoplasts in a buffered sorbitol medium the activities of proteases and RNase increase significantly. A corresponding increase of lysosomal aminopeptidase activity occurs in the absence of glutamic acid or casein hydrolysate. Cycloheximide and actinomycin D inhibit the increase of lysosomal hydrolase activities in differentiating protoplasts. The observed changes of enzyme activities are probably due to induced synthesis of the respective proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00397903
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  • 8
    Electronic Resource
    Electronic Resource
    Atmung und Wachstum von Hefe im Licht (1962)
    Springer
    Planta 58 (1962), S. 154-163 
    Details
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Summary Illumination of yeast cells with strong visible light results in an inhibition of the aerobic glucose metabolism. However, under these conditions the carbon dioxide output remains constant, so that the RQ rises to high values in time. The absorption bands of the cytochromes show that these pigments are reduced by light. The conclusion is that light inhibits the Krebs cycle. It also causes a strong inhibition of yeast growth.
    Notes: Zusammenfassung Belichtung von Hefezellen mit starkem sichtbarem Licht verursacht eine Hemmung des aeroben Umsatzes von Glucose. Unter den gleichen Bedingungen bleibt jedoch die CO2-Abgabe konstant, so daß sich hohe RQ-Werte ergeben. Die Absorptionsbanden der Cytochrome zeigen, daß diese Pigmente im Licht in den reduzierten Zustand übergehen. Es wird auf eine lichtbedingte Hemmung des Krebscyclus geschlossen. Das Wachstum der Hefe ist im Licht ebenfalls stark beeinträchtigt.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01911214
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  • 9
    Electronic Resource
    Electronic Resource
    Über die Lichtatmung der Brakteen vonEuphorbia pulcherrima (1962)
    Springer
    Planta 58 (1962), S. 193-198 
    Details
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Summary The red bracts of the spurgeEuphorbia pulcherrima (Poinsettia) undergo a pronounced and persistent decrease of their respiration by light. The amount of this inhibition is equivalent to the respiratory decrease caused by malonic acid. In bracts poisened by malonic acid no additional obstruction of the respiration occurs with light. It is concluded, that light intervenes with the Krebs cycle.
    Notes: Zusammenfassung Die roten Hochblätter vonEuphorbia pulcherrima erfahren im Licht eine starke und andauernde Hemmung der Atmung. Das Ausmaß dieser Atmungsbehinderung stimmt mit der durch Malonsäure erreichbaren Hemmung überein. Malonsäure-gehemmtes Blattgewebe erfährt im Licht keine zusätzliche Hemmung. Es wird gefolgert, daß im Licht der Ablauf des Krebscyclus unterbunden wird.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01911217
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  • 10
    Electronic Resource
    Electronic Resource
    Vacuolation: Origin and development of the lysosomal apparatus in root-tip cells (1968)
    Springer
    Planta 80 (1968), S. 159-175 
    Details
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The morphology of vacuolation has been investigated in root tip cells of corn using the freeze-etching technique. The genesis of vacuoles involves the following processes: a) Formation of small, endoplasmic-reticulum (ER)-derived vesicles (provacuoles); b) fusion of provacuoles resulting in the formation of small vacuoles, and followed by fusion and expansion of vacuoles; c) incorporation of large, dictyosome-derived vesicles into vacuoles by invagination of the tonoplast; d) invagination of the tonoplast resulting in the incorporation of cytoplasmic material into vacuoles. The morphological findings are correlated with biochemical data obtained from isolated vacuoles (lysosomes). Provacuoles (ER-derived vesicles) are shown to be primary lysosomes; their hydrolases arise from the ER. Vacuoles represent secondary lysosomes (digestive vacuoles) of the higher-plant cell. The metabolic role of lytic processes proceeding in the lysosomal apparatus is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385592
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