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  • 1
    Electronic Resource
    Electronic Resource
    Relationships between newly synthesized proteins and DNA puff patterns in salivary glands of Rhynchosciara americana (1977)
    Springer
    Chromosoma 61 (1977), S. 193-206 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract An analysis of the polypeptides synthesized in the salivary glands of late fourth instar R. americana larvae was accomplished by the use of electrophoresis followed by fluorography of in vitro labelled proteins. It was possible to detect five polypeptides which are synthesized only when the giant DNA puffs occur. One of these polypeptides was tentatively assigned to DNA puff B2 and another to the DNA puff C3. This assignment was based on correlations of polypeptide labelling, puffing patterns and RNA synthesizing capacity of the puffs in different sections of the gland during development. The possible meanings and implications of these DNA puffs are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00292804
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  • 2
    Electronic Resource
    Electronic Resource
    Fine structure and peroxidatic activity of rat blood monocytes (1977)
    Springer
    Cell & tissue research 185 (1977), S. 1-16 
    Details
    ISSN: 1432-0878
    Keywords: Monocyte (Rat) ; Type of granules ; Peroxidase ; Catalase ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Rat blood monocytes contain granules of two types, primary and secondary granules, which can be distinguished on morphological grounds. Secondary granules have a halo between the limiting membrane and the matrix; primary granules have no halo and are generally more elongate than the secondary granules. In addition, there are differences in the distribution of the diameters of these two types of granules. The cytochemical characteristics of the peroxidatic activity of these granules were investigated by using different prefixation conditions in combination with a number of DAB media differing in pH and H2O2 concentration. Prolongation of the prefixation and increasing the pH of the incubation medium increased the staining intensity of the secondary granules and decreased the staining intensity of the primary granules. In unfixed cells peroxidatic activity could be demonstrated only in the primary granules. It is concluded from the present findings that peroxidase occurs in primary granules and not in secondary granules, whereas catalase is present in secondary granules but probably not in primary granules. No evidence was found for the presence of microperoxisomes in rat blood monocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226665
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  • 3
    Electronic Resource
    Electronic Resource
    The differentiation of monocytes into macrophages, epithelioid cells, and multinucleated giant cells in subcutaneous granulomas (1979)
    Springer
    Cell & tissue research 197 (1979), S. 379-396 
    Details
    ISSN: 1432-0878
    Keywords: Peroxidatic activity ; Monocytes ; Macrophages ; Epithelioid cells ; Multinucleated giant cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The peroxidatic (PO) activity of monocytes differentiating into macrophages, epithelioid cells, and multinucleated giant cells in subcutaneous granulomas was investigated with three different media for the demonstration of PO activity. Irrespective of the stage of differentiation, these cells did not show PO activity in the rough endoplasmic reticulum (RER) or nuclear envelope. In addition, it was found that the morphologically characteristic types of granule of the various cells of the monocyte line (the primary granules and secondary granules of monocytes, the macrophage granules, and the epithelioid cell granules), all have distinct cytochemical characteristics. Monocytes lose their primary and secondary granules during differentiation into mature macrophages. Simultaneously, the granules of both types become elongated and the secondary granules lose their halo. In contrast to monocytes, mature macrophages may contain a few microperoxisomes. During the differentiation of macrophages into epithelioid cells or multinucleated giant cells there is an increase in the number of microperoxisomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233564
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  • 4
    Electronic Resource
    Electronic Resource
    The differentiation of monocytes into macrophages, epithelioid cells, and multinucleated giant cells in subcutaneous granulomas (1979)
    Springer
    Cell & tissue research 197 (1979), S. 355-378 
    Details
    ISSN: 1432-0878
    Keywords: Monocytes ; Macrophages ; Epithelioid cells ; Multinucleated giant cells ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The morphological changes occurring in monocytes during their differentiation into macrophages, epithelioid cells, Langhans-type giant cells, and foreign-body-type giant cells were investigated in foreign-body granulomas induced by subcutaneous implantation of pieces of Melinex plastic. Analysis based on Adams's (1974) criteria for discrimination between the several types of cell of the monocyte line, showed that each type has a characteristic type of granule. Primary and secondary granules, numerous in the Golgi area of monocytes were generally found close to the cell membrane and decreased in number in maturing macrophages. This was accompanied by an increase in the number of microtubules. Mature macrophages show numerous characteristic macrophage granules, which are round (average diameter: 280 nm) and have a halo between the limiting membrane and granular matrix. Mature epithelioid cells have characteristic epithelioid cell granules, and multinucleated giant cells a heterogeneous population of granules. Fusing macrophages generally have their Golgi areas facing each other, and also show a reduced thickness of the cell coat. The morphology of the multinucleated giant cell is closely related to the number of nuclei present. In Langhans-type giant cells, which generally have two to ten nuclei, a giant centrosphere with numerous aggregated centrioles is found. In transition forms between Langhans-type and foreign-body-type giant cells, which generally contain 10–30 nuclei, the centrioles show less aggregation. In the foreign-body-type giant cells, which generally have more than 30 nuclei, centrioles are virtually absent and never aggregated. These differences between the Langhans-type giant cells, the foreign-body-type giant cells, and the transition forms, support our previous finding that Langhans-type giant cells are the precursors of foreign-body-type giant cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233563
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  • 5
    Electronic Resource
    Electronic Resource
    Comparative study on peroxidatic activity in inflammatory cells on cutaneous and peritoneal implants (1979)
    Springer
    Cell & tissue research 197 (1979), S. 397-412 
    Details
    ISSN: 1432-0878
    Keywords: Peroxidatic activity ; Macrophages ; Multinucleated giant cells ; Skin ; Peritoneal cavity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Inflammatory reactions were evoked by simultaneous implantation of pieces of Melinex plastic in the subcutaneous tissues of the dorsum and in the peritoneal cavity of rats. The cellular composition of the Melinex-adherent cells and their peroxidatic (PO) activity were investigated in relation to the duration of implantation. Several striking differences were found between the subcutaneous and peritoneal implants. On the 7th and 14th days, multinucleated giant cells were abundantly present on the subcutaneous implants, whereas they were relatively rare on the peritoneal implants. The subcutaneous implants bore no mast cells and only a few eosinophilic granulocytes, but both types of cell were observed frequently on the peritoneal implants. Macrophages and multinucleated giant cells on the subcutaneous implants show PO activity only in the granules or are PO negative. On the peritoneal implants three types of macrophages can be distinguished: exudate macrophages which have PO activity restricted to granules or are PO-negative; macrophages with PO activity in granules and both the rough endoplasmic reticulum (RER) and nuclear envelope; and resident macrophages with PO activity only in the RER and nuclear envelope. In addition, two types of multinucleated giant cells are found, one with and the other without PO activity in the RER and nuclear envelope. Multinucleated giant cells with PO activity in the RER and nuclear envelope as well as exudate macrophages with PO activity in the RER and nuclear envelope were mainly found 32 h and 3 days after implantation of the Melinex in the peritoneal cavity. These findings are discussed in the light of current knowledge of the PO activity in macrophages and multinucleated giant cells. It is concluded that the appearance of PO activity in the RER and nuclear envelope of exudate macrophages and multinucleated giant cells is in all probability a transient phenomenon, and that there is no objective evidence to support the opinion that exudate macrophages with PO activity in the RER and nuclear envelope are transitional cells between exudate and resident macrophages.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233565
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  • 6
    Electronic Resource
    Electronic Resource
    The abdominal muscle receptor organ in Astacus leptodactylus (Crustacea) (1979)
    Springer
    Cell & tissue research 202 (1979), S. 41-61 
    Details
    ISSN: 1432-0878
    Keywords: Abdominal muscle receptor organ ; Dendrites ; Fine structure ; Crayfish
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The structure of both the slow- and the fast-adapting abdominal muscle receptor organ of Astacus leptodactylus is described with particular reference to differences between the two systems. The receptors are composed of a thin muscle that extends from the front edge of one segment to the front edge of the following and a sensory cell connected with this muscle. In the zone where the sensory cells enter their respective muscle, muscle fibers are reduced (zone of relative muscle exclusion=ZRME) and partly replaced by connective tissue. The occurrence of dendritic processes of both the slow and the fast neurons is confined to this zone. The following differences between the two receptor types are established: (1) The fast receptor muscle reveals a smaller sarcomere length than the slow receptor muscle and a higher myosin/actin filament ratio. (2) Muscle fibers that pass the ZRME are always found at its periphery in the fast system, separated from dendritic processes by layers of connective tissue, while in the slow system muscle fibers frequently are intermingled with the sensory elements. (3) The ZRME of the slow receptor is 20–30% longer than that of the fast receptor. (4) The dendritic varicosities of the slow neuron, on an average, contain many more mitochondria than those of the fast neuron. (5) Dendritic processes (fine twigs as well as varicosities) are juxtaposed to the sarcolemma of the muscle fibers only in the slow system; in the fast system dendrites and muscle are spatially separated by connective tissue. It is assumed that these differences between the two receptor types arep at least in part responsible for the different thresholds observed in physiological experiments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00239220
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  • 7
    Electronic Resource
    Electronic Resource
    Ultrastructural findings on lipoproteinsin vitro and in xanthomatous tissue (1978)
    Springer
    Journal of molecular histology 10 (1978), S. 299-307 
    Details
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Synopsis The application of OsO4 plus K3[Fe(CN)6] as a secondary fixative following aldehyde fixation, permitted demonstration of the presence of 30–300 nm ‘membrane-bound’ particles in xanthomatous tissue. With the same fixation method, isolated low density lipoprotein particles in a fibrin matrix could be observed in the transmission electron microscope in a way permitting comparison with similarly fixed tissue. However, isolated particles of very low density lipoproteins treated in the same way as low density particles had an irregular appearance and a diameter varying between 30 and 80 nm.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01007561
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