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  • Artikel: DFG Deutsche Nationallizenzen  (3)
  • Corticoids  (2)
  • 14C-Glucose  (1)
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  • Artikel: DFG Deutsche Nationallizenzen  (3)
Materialart
Erscheinungszeitraum
  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 280 (1973), S. 49-62 
    ISSN: 1432-1912
    Schlagwort(e): Corticotropin-Releasing Factor ; Corticoids ; Median Eminence ; Rat ; Histology
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary In 465 female Wistar rats the influence of an i.m. injection of various natural or synthetic corticoids on the augmentation of “Gomori-positive” granules in the outer layer of the median eminence following bilateral adrenalectomy has been investigated. While the augmentation of the granules is not impaired by progesterone it is inhibited or completely blocked, depending on the dose and the manner of application, by 11-desoxycorticosterone, corticosterone, cortisol, 9α-fluorocortisol, prednisolone, triamcinolone, dexamethasone and fluoroformylone. The doses necessary to suppress the augmentation of the granules correspond to those shown by other authors to be required for blocking ACTH-secretion. The inhibitory effect of the investigated corticoids on the augmentation of the granules is related to their antiphlogistic potency. The findings suggest that the amount of the “Gomori-positive” granules in the outer layer of the median eminence of the rat might be used as a parameter of the activity of the corticotropin-releasing factor.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Anatomy and embryology 146 (1974), S. 43-55 
    ISSN: 1432-0568
    Schlagwort(e): Pantothenic acid deficiency ; Corticotropin-releasing factor ; Corticoids ; Histologie ; Rat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary In the outer layer of the rat median eminence “Gomori-positive” granules occur which are assumed to be the morphological correlate of the corticotropin-releasing factor (CRF). The present study was carried out in order to establish whether the amount of these CRF-granules is influenced by a pantothenic acid (PA)-deficiency, which is known to result in an inhibition of corticosteroid production. Female SPF Wistar rats were used. PA-deficiency was induced by PA-free nutrition and/or administration of the PA-antagonist Ω-methyl-PA. Corresponding experimental groups were given either tap water or a solution of 1% NaCl and 5% glucose in tap water to drink. In rats fed with a diet free of PA and in addition treated with Ω-methyl-PA a severe disturbance of the corticoid balance developed within 6 weeks. The corticosterone production was depressed to about 11–16% of the control values. The adrenal cortex showed necrotic changes which either involved the whole parenchyma or were confined to the inner zones. In those animals in which a subcapsular region, in its extent corresponding to the zona glomerulosa, remained intact, an augmentation of the CRF-granules was observed. In those animals, however, in which the whole adrenal cortex was damaged, the amount of CRF-granules did not increase. Administration of the 1% NaCl containing drinking fluid did not influence the augmentation of the CRF granules, but had a protective effect on the development of necrotic changes in the zona glomerulosa of the adrenal cortex. The findings show that a functional relationship exists between the inner zones of the adrenal cortex and the CRF-granules in the median eminence.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    Archives of microbiology 100 (1974), S. 243-252 
    ISSN: 1432-072X
    Schlagwort(e): Saccharomyces cerevisiae ; Carbon Monoxide Trace-Measurement ; 14C-Glucose ; CO Production ; Atmospheric Cycle of Trace Gases
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung 1. Mit einer empfindlichen Analysenmethode, die auf die Reaktion CO+HgO→CO2+Hg basiert und den CO-Gehalt auf Grund der Absorption des freigesetzten Hg bei 2537 Å ermittelt, wurden im Gasraum über wachsenden Kulturen von Saccharomyces cerevisiae, S. oviformis, Escherichia coli, Aerobacter aerogenes, Pseudomonas spec. und Lactobacillus brevis 0.4–2.6 ppm CO nachgewiesen. Bei Lactobacillus arabinosus, Bacillus cereus var. mycoides und Aspergillus niger war eine CO-Bildung nicht meßbar. 2. Bei S. cerevisiae war die CO-Bildung bei Konzentrationen von 10–50 g Glucose pro Liter Medium am größten. Außerdem wurde die CO-Bildung proportional zum anfänglichen Sauerstoffgehalt im Gasraum über den Kulturen gefördert. 3. Mit 14C-markierter Glucose wurde nachgewiesen, daß CO aus Glucose entsteht. 4. Die CO-Bildung der untersuchten Mikroorganismen ist so gering, daß sie keine Bedeutung für den Kreislauf dieses Spurengases in der Atmosphäre hat.
    Notizen: Summary 1. Growing cultures of Saccharomyces cerevisiae, S. oviformis, Escherichia coli, Aerobacter aerogenes, Pseudomonas spec. and Lactobacillus brevis produce trace amounts of CO (0.4–2.6 ppm) that can be detected in the gas space above the cultures using a sensitive analytical method based on the reaction CO+HgO→CO2+Hg. The liberated Hg is quantitatively measured by atomic absorption at 2537 Å. No CO could be detected above cultures of Lactobacillus arabinosus, Bacillus cereus var. mycoides and Aspergillus niger. 2. The maximum CO production by Saccharomyces was obtained with concentrations of 10–50 g glucose per liter medium. The amount of CO formed increased with the oxygen concentration in the gas space above the cultures. 3. Using 14C-glucose it was shown that S. cerevisiae forms CO from glucose. 4. The formation of CO by the microorganisms investigated is very small. The ratio of CO/CO2 produced is much smaller than in environmental air. Therefore it can be concluded that the production of CO by these microorganisms has probably no significance for the atmospheric cycle of this trace gas.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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