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  • 1
    Electronic Resource
    Electronic Resource
    Comparative platelet anti-aggregant activity of D-cysteinolic acid analogues (1989)
    Springer
    Cellular and molecular life sciences 45 (1989), S. 1110-1112 
    Details
    ISSN: 1420-9071
    Keywords: D-Cysteinolic acid ; platelet aggregation ; inhibitor ; marine product ; 2-aminoethyl disulfide ; sardine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary D-Cysteinolic acid (1) analogues with an S-C-C-N skeleton showed increased platelet anti-aggregant activity in the following order: 2-aminoethanesulfonic acids, thiazolidines, 2-aminoethanethiols and 2-aminoethyl disulfides. Methyl substitutions at the 2-position potentiated the activity. Of these analogues, bis [(R)-2-aminopropyl] disulfide was the most potent inhibitor of platelet aggregation, with about 600-fold the activity of (1).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01950172
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Green Fluorescent Protein (GFP) as a Marker for Cell Viability After UV Irradiation (1999)
    Springer
    Journal of fluorescence 9 (1999), S. 37-43 
    Details
    ISSN: 1573-4994
    Keywords: Green fluorescent protein (GFP) ; constitutive GFP expression ; visualization of GFP fluorescence ; UV exposure of mammalian cells ; Chinese Hamster Ovary (CHO) cell lines of different repair capabilities
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Generation of Chinese Hamster Ovary (CHO) cell lines stably expressing green fluorescent protein (GFP) was achieved using a plasmid vector that encoded the red-shifted pCX-xGFP under the control of a strong hybrid promoter composed of a CMV enhancer and a β-actin/β-globin gene promoter. Cotransfection of the promoter-less pSV2-Neo helper plasmid transmitting neomycin resistance was followed by selection with the antibiotic G418. Constitutive GFP expression could be visualized in living and fixed cells using fluorescence spectroscopy, fluorescence microscopy, and flow cytometry. DNA repair-proficient (AA8) and deficient (UV5) CHO strains were used for survival tests after UVC irradiation. Cells carrying the GFP construct (AA8-pGFP, UV5-pGFP) show the same response to UV irradiation (colony forming ability) as their nontransformed parental cell lines (AA8, UV5). Using GFP as a marker for cell viability, cells were harvested after certain postirradiation growth periods and the numbers of GFP expressing cells and fluorescence intensities were determined by FACS analysis. Generally, GFP fluorescence in irradiated cells is not seen when cell membranes are damaged (leak-out of the soluble GFP). Irradiated cells without membrane damage express GFP continuously (leading to a dose-dependent increase in GFP contents).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1020583623407
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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