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  • 21.60.Fw  (2)
  • DNA markers  (2)
  • 23.20.−g  (1)
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  • 1
    Digitale Medien
    Digitale Medien
    Detection of DNA sequence polymorphisms among wheat varieties (1992)
    Springer
    Theoretical and applied genetics 84 (1992), S. 573-578 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Common wheat ; RAPD ; DGGE ; DNA polymorphism ; DNA markers
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary A DNA marker detection strategy that allows the rapid, efficient resolution of high levels of polymorphism among closely related lines of common wheat (Triticum aestivum) has been developed to circumvent the apparent lack of restriction fragment length polymorphism in many important self-pollinated crop species. The technique of randomly amplified polymorphic DNA (RAPD) was combined with a denaturing gradient gel electrophoresis system (DGGE) to explore DNA sequence polymorphisms among different genotypes of wheat. Of the 65 primer combinations used for the polymerase chain reaction (PCR) amplifications, over 38% of them produced readily detectable and reproducible DNA polymorphisms between a spring wheat line, SO852, and a winter wheat variety, ‘Clark’. A high level of polymorphism was observed among a number of commercial varieties and breeding lines of wheat. This procedure was also used to detect polymorphisms in a recombinant inbred population to test the feasibility of its application in genome mapping. This DNA polymorphism detection system provides an opportunity for pedigree analysis and fingerprinting of developed wheat lines as well as construction of a high density genetic map of wheat. Without the need for 32P and sophisticated DNA extraction procedures, this approach should make it feasible to utilize marker-based selection in a plant breeding program.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00224154
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  • 2
    Digitale Medien
    Digitale Medien
    Association of a DNA marker with Hessian fly resistance gene H9 in wheat (1994)
    Springer
    Theoretical and applied genetics 89 (1994), S. 964-968 
    Details
    ISSN: 1432-2242
    Schlagwort(e): RAPD ; Wheat ; Hessian fly ; DNA markers
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The Hessian fly [Mayetiola destructor (Say)] is a major pest of wheat (Triticum aestivum L.) and genetic resistance has been used effectively over the past 30 years to protect wheat against serious damage by the fly. To-date, 25 Hessian fly resistance genes, designated H1 to H25, have been identified in wheat. With near-isogenic wheat lines differing for the presence of an individual Hessian fly resistance gene, in conjunction with random amplified polymorphic DNA (RAPD) analysis and denaturing gradient-gel electrophoresis (DGGE), we have identified a DNA marker associated with the H9 resistance gene. The H9 gene confers resistance against biotype L of the Hessian fly, the most virulent biotype. The RAPD marker cosegregates with resistance in a segregating F2 population, remains associated with H9 resistance in a number of different T. aestivum and T. durum L. genetic backgrounds, and is readily detected by either DGGE or DNA gel-blot hybridization.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00224525
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  • 3
    Digitale Medien
    Digitale Medien
    Structure of theN=59 nucleus97Sr: coexistence of spherical and deformed states (1990)
    Springer
    The European physical journal 337 (1990), S. 149-159 
    Details
    ISSN: 1434-601X
    Schlagwort(e): 23.20.Lv ; 21.60.Ev ; 23.20.−g
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract A band with a rotational pattern based on a state at 585.1 keV has been identified in theN=59 neutron-rich nucleus97Sr. Its properties lead to the [422] 3/2 Nilsson-orbital assignment for the band head. There is evidence for a second band with the head at 644.7 keV and the configuration [541] 3/2. Since the ground state and the lowest excited levels are spherical, shape coexistence is established for97Sr. A deformed nature of several levels at 500–600 keV results also from QRPA-model calculations. The structure of the low-lying spherical levels has been studied in the frame of the IBF model. The results of the present investigations lead to a better understanding of theN=59 isotones which constitute the link between the spherical and deformed nuclei atA∼100 as a species with shape coexistence but without any indications of particular softness.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01294286
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  • 4
    Digitale Medien
    Digitale Medien
    Structure of the neutron-rich nucleus 37 94 rb57 (1989)
    Springer
    The European physical journal 334 (1989), S. 259-267 
    Details
    ISSN: 1434-601X
    Schlagwort(e): 21.10.Pc ; 21.60.Fw ; 27.60.+j
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract A level scheme has been established for 37 94 Rb57 based on studies at the fission-product separator JOSEF ofγγ coincidences following theβ- decay of94Kr. For the 190.7 keV level a half-life of 2.5(8) ns has been determined through the centroid-shift method. The majorβ-decay branch of94Kr proceeds with log ft=3.7 into the 988.4 keV state in94Rb. This transition must be of the 0+→1+ GT type involving the transitionνg7/2→πg9/2. IBFFM calculations have been performed for94Rb, starting from the parametrization used in the previous IBM investigations for the isotope93Rb and the isotone96Y. A theoretical interpretation of the low-lying levels including propositions for the spins is given in a comparison to the available data.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01284553
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  • 5
    Digitale Medien
    Digitale Medien
    Structure of low-lying levels in91Rb and93Rb (1986)
    Springer
    The European physical journal 325 (1986), S. 139-147 
    Details
    ISSN: 1434-601X
    Schlagwort(e): 23.20.Ck ; 21.60.Fw ; 27.60.+j
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract Life-times of low-lying levels in 37 91 Rb54 and 37 93 Rb56 have been determined fromβ −−γ coincidence measurements at the fission-product separator JOSEF. Values oft 1/2=17.0(8) ns and 2.0(2) ns have been obtained for the levels at 1,134 keV in91Rb and 267 keV in93Rb, respectively, and upper limits could be deduced for several other states. Calculations in the frame of IBFM/PTQM have been performed for91Rb, the results of which allow an interpretation of the low-lying levels of this nucleus. For the 1,134 keV level the configuration [πg 9/2⊗2+] 7/2+ is suggested which lies close to or even below the 9/2 1 + level. The calculated half-life of 14 ns for the 1,134 keV level reproduces well the experimental value. The half-life of the 267 keV level in93Rb favours the assignment ofI π=1/2− to this state over the alternative 3/2−.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01289644
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