ISSN:
1432-0983
Keywords:
Multi-copy plasmid
;
Hyper-resistance
;
4-NQO
;
MNNG
;
Triaziquone
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Screening of a multi-copy vector-based yeast genomic library in haploid cells of wild-type Saccharomyces cerevisiae yielded transformants hyper-resistant to various chemical mutagens. Genetical analysis of the yeast insert DNAs revealed three genes SNG1, SNQ2, and SNQ3 that confer the phenotype hyper-resistance to MNNG, to 4-NQO and triaziquone, and to mutagens 4-NQO, MNNG, and triaziquone, respectively. Integration of the gene disruption-constructs into the haploid yeast genome yielded viable null-mutants with a mutagen-sensitive phenotype. Thus, copy number of these non-essential yeast genes determines the relative resistance to certain chemical mutagens, with zero copies yielding a phenotype of mutagen sensitivity and multiple copies one of mutagen hyper-resistance, respectively.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00351689
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