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Digitale Medien

Isolation of β-Xylanase from whole broth of Bacillus amyloliquefaciens by adsorption on a matrix in fluidized bed with low degree of expansion (1999)

Breccia, Javier D. ; Hatti-Kaul, Rajni ; Castro, Guillermo R. ; [weitere]

Springer

Bioseparation 8 (1999), S. 273-280

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ISSN: 1573-8272

Schlagwort(e): β-1 ; 4-xylanase ; Bacillus amyloliquefaciens ; cation exchanger ; fluidized bed adsorption ; low bed expansion

Quelle: Springer Online Journal Archives 1860-2000

Thema: Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: Abstract β-1,4-Xylanase, produced extracellularly by Bacillus amyloliquefaciens MIR 32, was isolated directly from the culture broth by adsorption on a cation exchanger, Amberlite IRC-50, in fluidized bed with a low degree of expansion. The enzyme was eluted from the adsorbent by increase in pH, with a recovery of 82.3% and purification of 5.3 fold. About 99.99% of the colony forming units, 82% of the contaminating neutral protease activity, and 100% of the reducing sugars present in the crude feedstock were removed at the end of the purification cycle.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1023/A:1008174513826

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Digitale Medien

Purification of recombinant protein A by aqueous two-phase extraction integrated with affinity precipitation (1992)

Kamihira, Masamichi ; Kaul, Rajni ; Mattiasson, Bo

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 40 (1992), S. 1381-1387

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ISSN: 0006-3592

Schlagwort(e): aqueous two-phase extraction ; affinity precipitation ; Eudragit ; protein A ; Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: Aqueous two-phase extraction incorporated affinity precipitation was examined as a technique for protein purification. An enteric coating polymer, Eudragit S100, was employed as a ligand carrier. Eudragit was specifically partitioned to the top phase in the aqueous two-phase systems. For application of this method to purification of recombinant protein A using human IgG coupled to Eudragit in an aqueous two-phase system, 80% of protein A added was recovered with 81% purity. The purity was enhanced 26-fold by thid method. The IgG-Eudragit could be used repeatedly for the purification process. This seperation method should be applicable to industrial-scale purification as a new purification procedure combining the advantages and compensating for the disadvantages of the aqueous two-phase method and affinity precipitation method. © 1992 John Wiley & Sons, Inc.

Zusätzliches Material: 5 Ill.