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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 135 (1983), S. 199-204 
    ISSN: 1432-072X
    Keywords: Rhizobium leguminosarum ; Nitrogenase ; Acetylene reduction ; Growth yields ; Chemostat ; Fedbatch
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Rhizobium leguminosarum is capable of nitrogen fixation in free living cultures. Nitrogenase activity can be induced when the ammonia supply of a nitrogen-limited culture, in which the oxygen concentration is regulated at 1 μm, is switched off. Assuming a nitrogen content of the cell of 12% the theoretical washout curve of such a culture can be calculated. The measured optical densities are higher than the calculated ones. In one case, 8 days after the shift, the medium supply was switched off and batch growth with molecular nitrogen occurred. Acetylene reduction started immediately in samples taken 2 days after the shift. Addition of ammonium chloride to the test vials inhibited acetylene reduction. The highest specific activities were found 5–8 days after the shift (100 nmol ethylene formed per milligram dry weight per hour). From this activity a μmax of 0.007–0.009 (generation time 76–98 h) on molecular nitrogen can be calculated. After the shift Y mannitol decreased from 33 to 23 in the first 48 h. Three days after the shift Y mannitol had a value of 15. During batch growth Y mannitol had a value of 8. In a carbon-limited fed-batch culture R. leguminosarum shows three distinct growth phases with different values of Y mannitol. This is an indication for a stringent response in the third growth phase, and probably nitrogen fixation occurs under stringent response situations.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Medical & biological engineering & computing 38 (2000), S. 632-638 
    ISSN: 1741-0444
    Keywords: Accelerometry ; Gait ; Kinematics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The use of piezo-resistive uni-axial accelerometer signals in gait analysis is complicated by the fact that the measured signal is composed of different types of acceleration. The aim of the study is to obtain insight into the signal from a tangential accelerometer attached to the thigh during walking. Six subjects walk with three different speeds. Simultaneous measurements are performed with accelerometers, footswitches and an opto-electronic system. The components of the accelerometer signal are calculated from the opto-electronic system. A clear relationship is found between the measured and calculated accelerometer signals (range RMS: 0.76–3.69 m s−2, range rms: 0.22–0.61). The most pronounced feature is a high positive acceleration peak (〉 10 m.s−2) at the end of the cycle. The gravitational acceleration during one cycle is characterised by a sinusoidal shape, whereas the inertial acceleration contains higher-frequency components (up to 20 Hz). During the major part of the gait cycle, the gravitational and inertial acceleration make opposing contributions to the signal. As a result, the gravitational acceleration influences the amplitudes of the measured acceleration signal, the shape and peaks of which are mainly determined by the inertial acceleration. Because the gravitational and inertial accelerations differ in frequency components, the application for gait analysis remains feasible.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1741-0444
    Keywords: Accelerometry ; Upper limb usage ; Mobility-related activities
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The aim of this research was to assess the ability of an upper limb-activity monitor (ULAM) to discriminate between upper limb usage and non-usage in healthy and disabled subjects during normal daily life. The ULAM was based on ambulatory accelerometry and consisted of several acceleration sensors connected to a small recorder worn around the waist. While wearing this ULAM, four healthy and four disabled subjects performed an activity protocol representing normal daily life upper limb usage or non-usage. The motility feature (derived from the raw acceleration signals) was used as a measure of the extent of upper limb usage. Agreement scores between ULAM output and videotape recordings (reference method) were calculated. ULAM data that were of special interest for rehabilitation were detected satisfactorily (overall agreement 83.9%). There were no systematic differences in the agreement percentages between healthy and disabled subjects for mobility-related activities (p=0.345) and the different forms of upper limb usage or non-usage (p=0.715). The ULAM can be used in future studies in subjects with upper limb disorders to discriminate between upper limb usage and non-usage during performance of mobility-related activities to determine activity limitations.
    Type of Medium: Electronic Resource
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