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A population analysis of Robertsonian and Ag-NOR polymorphisms in brown trout (Salmo trutta) (1994)

Castro, J. ; Rodríguez, S. ; Arias, J. ; [et al.]

Springer

Theoretical and applied genetics 89 (1994), S. 105-111

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ISSN: 1432-2242

Keywords: Salmo trutta ; Population analysis Robertsonian polymorphism ; Ag-NOR pattern

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract An analysis of Robertsonian polymorphism and variation in the number of active NORs has been carried out in several populations of brown trout (Salmo trutta) from Northwestern Spain. The karyotype of this species appears to be soundly established, and essentially no variation has been found in chromosome number. Interindividual and interpopulation variation in arm number was detected, with figures ranging between 100 and 102 among individuals, and between 100.10 and 100.80 among populations. This variation in arm number is solely attributable to the polymorphism of the short arm of the main NOR-bearing pair 11, which can appear from acrocentric to metacentric in different individuals. Most populations analyzed showed the standard distribution of active NORs previously observed in this species. The Miño drainage basin, and specially the Chamoso population, showed a multi-chromosomal distribution of active NORs, with several new locations, always telomeric. In most cases no concordance was observed between previously detected rDNA sites in S. trutta and the new Ag-NOR locations. This fact suggests a transposition mechanism rather than an activation of silent rDNA sites to explain this multichromosomal NOR pattern.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00226990

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Electrochemical study of iproniazid and its analytical determination by differential pulse polarography (1994)

Martín, M. E. ; Borges, T. M. ; Arias, J. J. ; [et al.]

Weinheim : Wiley-Blackwell

Electroanalysis 6 (1994), S. 491-496

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ISSN: 1040-0397

Keywords: Iproniazid ; Differential pulse polarography ; Chemistry ; Polymer and Materials Science

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology

Notes: Iproniazid was found to be irreversibiy reduced in two steps, involving the exchange of two electrons and two protons each, by using tast polarography, normal pulse polarography (NPP), and cyclic voltammetry (CV). The rate constants of these two steps were found to be 5.8 × 10-6 and 4.2 × 10-6 and 4.2 × 10-6 cm s-1, respectively, and their corresponding diffusion coefficients 4.5 × 10-5 and 8.3 × 10-5 cm2 s-1, Also, α, the coefficient of cathodic transfer, was found to be 0.20 to 0.25 for the first peak and 0.17 to 0.15 for the second. On the basis of the electrochemical study performed, a differential pulse polarographic method for the determination of iproniazid over the concentration range of 5 × 10-7 M to 1 × 10-4 M was developed.

Additional Material: 7 Ill.