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  • Somaclonal variation  (2)
  • Allosteric model Binding Gating Neuronal nicotinic receptors Single channel  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Gating of α3β4 neuronal nicotinic receptor can be controlled by the loop M2-M3 of both α3 and β4 subunits (1999)
    Springer
    Pflügers Archiv 439 (1999), S. 86-92 
    Details
    ISSN: 1432-2013
    Keywords: Allosteric model Binding Gating Neuronal nicotinic receptors Single channel
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Previous studies have shown that the gating mechanism of α3β4 neuronal nicotinic receptors is affected by a residue in the middle of the M2-M3 loop of the β4 subunit. We have extended the study of the same location to the α3 subunit. Bovine α3β4 receptors were mutated in position 268, substituting the residue present in wild-type receptors, i.e. leucine in α3 and asparagine in β4, for an aspartate. Wild-type and mutated α3 and β4 subunits were combined to form four different receptors. We have measured macroscopic currents in Xenopus oocytes elicited by nicotine, and related them to surface receptor expression measured with an epibatidine-binding essay. We also obtained single-channel recordings of the receptors to study their kinetic behaviour. The results were analysed in terms of an allosteric model with three states. We found that the effect of the mutation in the α3 subunit on the gating of the receptor was similar to the corresponding mutation in the β4 subunit. The effect when both subunits were mutated was additive, suggesting that the contribution of each subunit to the gating mechanism is independent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004249900143
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Somaclonal variation versus chemically induced mutagenesis in tomato (Lycopersicon esculentum L.) (1987)
    Springer
    Theoretical and applied genetics 74 (1987), S. 733-738 
    Details
    ISSN: 1432-2242
    Keywords: Tomato ; Somaclonal variation ; Chemical mutagenesis ; Mutants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A comparison was made of the type and frequency of mutational events found in the progeny of tomato plants regenerated after one passage in vitro with those induced by chemical mutagenesis with ethyl methane sulphonate. Several mutants were recovered in the progeny of regenerated and mutagenized plants of two cultivars of tomato. They can be grouped into the following categories: seedling lethality, male sterility, resistance to Verticillium, short stature, change in number of lateral shoots or in leaf shape. The results indicate that the two sources of variability differ in their effect, changing the spectrum and frequency of the mutants as well as, at least in some cases, their pattern of segregation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00247550
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    The histogenesis of somaclones from tomato (Lycopersicon esculentum Mill.) cotyledons (1988)
    Springer
    Protoplasma 142 (1988), S. 156-163 
    Details
    ISSN: 1615-6102
    Keywords: Tomato ; Lycopersicon esculentum ; Somaclonal variation ; Plant regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A histological study ofin vitro cultured cotyledonary expiants of tomato (Lycopersicon esculentum) was performed in order to determine the site (differentiated tissue or developing callus) and the mode of plant regeneration. Results have shown that callus develops at the excision sites of cotyledonary expiants and that shoots are formed exclusively within the unorganized callus: excision areas are the only morphogenetic sites and the proximal excision is the preferred site for plant regeneration. Shoots differentiate by organogenesis within the superficial region of the callus. Few neocambial cells cooperate in the neoformation. Origin from a single cell is highly unlikely since rarely observed single activated cells never developed into shoots. Regenerated plants may be chimeras if invitro culture induces genetic diversity in the initial cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01290872
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    Paper (German National Licenses)
    Fulltext
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