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  • Analytical Chemistry and Spectroscopy  (12)
  • Oryza sativa  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Senescence of rice leaves XXIX. Ethylene production, polyamine level and polyamine biosynthetic enzyme activity during senescence
    Amsterdam : Elsevier
    Plant Science 78 (1991), S. 193-198 
    Details
    ISSN: 0168-9452
    Keywords: Oryza sativa ; S-adenosyl-l-methionine decarboxylase ; arginine decarboxylaase ; ethylene ; leaf senescence ; ornithine decarboxylase ; putrescine ; spermidine ; spermine
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9452(91)90198-H
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  • 2
    Electronic Resource
    Electronic Resource
    Salicylic acid inhibits the biosynthesis of ethylene in detached rice leaves (1993)
    Springer
    Plant growth regulation 12 (1993), S. 79-82 
    Details
    ISSN: 1573-5087
    Keywords: ethylene biosynthesis ; salicylic acid ; Oryza sativa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effects of salicylic acid (SA) on ethylene biosynthesis in detached rice leaves were investigated. SA at pH 3.5 effectively inhibited ethylene production within 2 h of its application. It inhibited the conversion of ACC to ethylene, but did not affect the levels of ACC and conjugated ACC. Thus, the inhibitory effect of SA resulted from the inhibition of both synthesis of ACC and the conversion of ACC to ethylene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00144586
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  • 3
    Electronic Resource
    Electronic Resource
    Changes in putrescine accumulation induced by agents affecting cytosolic pH in detached rice leaves (1993)
    Springer
    Plant growth regulation 13 (1993), S. 133-136 
    Details
    ISSN: 1573-5087
    Keywords: cytosolic pH ; Oryza sativa ; putrescine ; rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Effects of compounds that influenced cytosolic pH on the level of putrescine in detached rice leaves were examined. Permeant weak acids, isobutyric acid and propionic acid, increased the level of putrescine in detached rice leaves. Procaine and trisodium citrate, known to be permeant weak bases, on the other hand, decreased the level of putrescine. It seems possible that the level of putrescine in detached rice leaves is regulated by the cytosolic pH.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00024255
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Mass spectrometry of laser-desorbed oligonucleotides (1992)
    New York, NY : Wiley-Blackwell
    Rapid Communications in Mass Spectrometry 6 (1992), S. 365-368 
    Details
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: Various matrices were used for laser-desorption ionization of mixtures of small DNA segments. Parent negative ions of 34-mer as well as other smaller single-stranded oligomers were observed for selected organic acids and dye compounds. Both positive and negative parent ions were observed with very little fragmentation. Effects of various matrices and the potential applications to DNA sequencing are briefly discussed.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/rcm.1290060602
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  • 5
    Electronic Resource
    Electronic Resource
    Laser mass spectrometry of oligonucleotides with isomer matrices (1993)
    New York, NY : Wiley-Blackwell
    Rapid Communications in Mass Spectrometry 7 (1993), S. 435-439 
    Details
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: Various isomer matrices were used for laser-desorption ionization of mixtures of oligomers. It was found that the efficiency of production of oligomer ions changed drastically when different isomers were used as matrices. For selected matrix materials, parent oligomer ions with sizes up to 64 bases were observed.
    Additional Material: 13 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/rcm.1290070606
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  • 6
    Electronic Resource
    Electronic Resource
    Picolinic acid as a matrix for laser mass spectrometry of nucleic acids and proteins (1994)
    New York, NY : Wiley-Blackwell
    Rapid Communications in Mass Spectrometry 8 (1994), S. 673-677 
    Details
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: We found that picolinic acid is a very good matrix for matrix-assisted laser desorption/ionization (MALDI) time-of-flight mass spectrometry of oligonucleotides, proteins and tRNA. Among the oligonucleotides, picolinic acid was shown to be effective for homo-oligonucleotides, d(G)40 and d(C)60, and for mixed-base oligonucleotides up to 190 bases. In the case of the single-stranded oligonucleotides, and of double-stranded ones as well, only parent ions corresponding to single-stranded DNA were observed. The efficiency for MALDI of oligonucleotides using the picolinic acid matrix was superior to that using 3-hydroxypicolinic acid. MALDI of transfer RNA phenylalanine-specific (tRNAphe), a 76-base ribonucleic acid, was detected with a signal-to-noise ratio of 〉10. By comparison with 3-hydroxypicolinic acid the results with picolinic acid are notably superior for all oligonucleotides.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/rcm.1290080902
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    3-aminopicolinic aid as a matrix for laser desorption mass spectrometry of biopolymers (1994)
    New York, NY : Wiley-Blackwell
    Rapid Communications in Mass Spectrometry 8 (1994), S. 1001-1006 
    Details
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: 3-Aminopicolinic acid (3-APA) was tested and found to be a useful matrix for matrix-assisted laser desorption/ionization of DNA and protein. Single-stranded DNA segments of 150-mer and double-stranded DNA of 246 base pairs were successfully detected by using 3-APA as an ultraviolet-absorbing matrix in a linear time-of-flight mass spectrometer. In the case of the double-stranded DNA, only parent ions corresponding to single-stranded DNA were observed. The comparison with 3-hydroxypicolinic acid and picolinic acid matrices is discussed.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/rcm.1290081219
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  • 8
    Electronic Resource
    Electronic Resource
    Detection of 500-nucleotide DNA by laser desorption mass spectrometry (1994)
    New York, NY : Wiley-Blackwell
    Rapid Communications in Mass Spectrometry 8 (1994), S. 727-730 
    Details
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: We report the first detection of DNA segments as large as 500 nucleotides by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry, using picolinic acid and 3-hydroxypicolinic acid mixtures as desorption matrices. The successful detection of 500-nucleotide DNA indicates that laser mass spectrometry is now emerging as a new biotechnology tool for DNA-related research. It should be possible to utilize fast detection of large DNA segments by laser mass spectrometry for rapid human genome sequencing.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/rcm.1290080913
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  • 9
    Electronic Resource
    Electronic Resource
    Revisit of MALDI for small proteins (1995)
    New York, NY : Wiley-Blackwell
    Rapid Communications in Mass Spectrometry 9 (1995), S. 1315-1320 
    Details
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: Matrix-assisted laser desorption/ionization (MALDI) was used for several small proteins (such as insulin) and for peptides. It was found that the detection efficiencies of MALDI for the insulin B chain and the insulin A chain are drastically different. Similar phenomena were also observed for various types of peptides. The positive-ion signal of MALDI in detecting proteins or peptides was found to be greatly enhanced by the presence of a basic amino acid in their chains. The experimental results indicate that this enhancement may arise from proton transfer in solution by an acid-base reaction between the protein/peptide and matrix molecule. This pre-protonated mechanism provides a low energy barrier for the ionization of peptides in a MALDI process, and greatly reduces the energy threshold of MALDI. Matrix effects on the ionization mechanism are discussed.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/rcm.1290091318
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  • 10
    Electronic Resource
    Electronic Resource
    Laser mass spectrometry of polydeoxyribothymidylic acid mixtures (1993)
    New York, NY : Wiley-Blackwell
    Rapid Communications in Mass Spectrometry 7 (1993), S. 63-66 
    Details
    ISSN: 0951-4198
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: Matrix-assisted laser desorption/ionization mass spectrometry has been used to mass analyze synthetic oligomers of poly-T ranging in size from 20 to 100 nucleotides. Both positively- and negatively-charged parent molecular ions were observed.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/rcm.1290070115
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