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Detection of 500-nucleotide DNA by laser desorption mass spectrometry (1994)

Tang, K. ; Taranenko, N. I. ; Allman, S. L. ; [et al.]

New York, NY : Wiley-Blackwell

Rapid Communications in Mass Spectrometry 8 (1994), S. 727-730

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ISSN: 0951-4198

Keywords: Chemistry ; Analytical Chemistry and Spectroscopy

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Physics

Notes: We report the first detection of DNA segments as large as 500 nucleotides by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry, using picolinic acid and 3-hydroxypicolinic acid mixtures as desorption matrices. The successful detection of 500-nucleotide DNA indicates that laser mass spectrometry is now emerging as a new biotechnology tool for DNA-related research. It should be possible to utilize fast detection of large DNA segments by laser mass spectrometry for rapid human genome sequencing.

Additional Material: 7 Ill.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1002/rcm.1290080913

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