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  • 1
    Electronic Resource
    Electronic Resource
    Homogeneous mono-125I-insulins (1980)
    Springer
    Diabetologia 19 (1980), S. 546-554 
    Details
    ISSN: 1432-0428
    Keywords: 125I-insulin ; insulin ; radioiodination ; iodate ; lactoperoxidase ; monoiodotyrosine ; diiodotyrosine ; A chain ; B chain ; tyrosine-A14 ; tyrosine-A19 ; insulin immunoassay ; radiochemical purity ; radioimmunochemical stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Mono-125I-(Tyr A19)-insulin (Mono *A19) was prepared by iodinating MC porcine insulin with 125I in acid medium using iodate (as oxidizing agent), followed by anion-exchange chromatography. Mono-125I-(Tyr A14)-insulin (Mono *A14) was prepared by iodinating MC porcine insulin with 125I, using H2O2/lactoperoxidase at neutral pH, followed by anion-exchange chromatography. The specific radioactivities were in the ranges of 120–200 and 220–300 mCi/mg for Mono *A19 and Mono *A14, respectively. Analyses of the intramolecular distributions of 125I demonstrated that the preparations were 97–98% radiochemically pure. In both preparations, 98–99% of the radioactivity was capable of binding to insulin antibodies for up to 6 months of storage of the tracers. The IRI concentration decreased with the duration of storage. The greatest observed fall in IRI concentration was 70%. The time course could be explained by the assumption that the disintegration of a 125I-nucleus destroys the immunoreactivity of the insulin molecule in which the decay occurs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00253183
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  • 2
    Electronic Resource
    Electronic Resource
    Intramuscular laser-Doppler flowmetry in the supraspinatus muscle during isometric contractions (1995)
    Springer
    European journal of applied physiology 71 (1995), S. 373-378 
    Details
    ISSN: 1439-6327
    Keywords: Microcirculation ; Shoulder ; Abduction ; Laser-Doppler
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract To study the regulation of microvascular blood flow in a compartment muscle, laser-Doppler measurements of muscle microcirculation were recorded in the supraspinatus muscle in eight volunteers during and following submaximal isometric muscle contractions. The subjects performed isometric shoulder abductions at five contraction levels from 5% to 50% maximal voluntary contraction for I min each and a sustained 30° shoulder abduction for 20 min. The subjects' perceived exertion increased from “no perceived exertion” to “near maximal exertion” during the 20-min period with 30° shoulder abduction. Microcirculation increased during all 1-min contractions. Following the contractions at 20%, 30% and 50% MVC post-exercise reactive hyperaemia was seen for a period of at least 1 min. The reactive hyperaemia increased in magnitude in response to increasing contraction level. The results showed the same time-history of the blood flow at microvascular level as previously seen in larger peripheral vessels in response to muscle contractions. During the 20-min contraction microcirculation increased in line with the findings during the brief contractions. However, in contrast to the brief contractions no postexercise reactive hyperaemia occurred following the prolonged contraction. Lack of postexercise reactive hyperaemia following the prolonged shoulder abduction would suggest insufficient regulation of the vascular resistance. Alternatively, lack of hyperaemia could be taken as an indication of sufficient microcirculation during the preceding contraction. From previous studies on intramuscular pressure and metabolism the latter alternative would seem unlikely.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00240420
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    Paper (German National Licenses)
    Fulltext
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