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Plasticity in the barrel cortex of the adult mouse: Effects of peripheral deprivation on GAD-immunoreactivity (1989)

Welker, E. ; Soriano, E. ; Loos, H.

Springer

Experimental brain research 74 (1989), S. 441-452

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ISSN: 1432-1106

Keywords: Barrel cortex ; GAD-immunoreactivity ; Adult cortical plasticity ; Somatosensory system ; Mouse

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Summary The whisker-to-barrel pathway of the adult mouse was used in a study on the effects of peripheral sensory deprivation on GAD-immunoreactivity in the somatosensory cortex. At varying periods of time after removal of a set of vibrissal follicles, mice were processed for immunohistochemistry using an antibody against GAD. In sections tangential to the cortical surface we observed, in the barrels whose follicles were removed, decreased immunoreactivity as early as three days after surgery. The decrease was due to a lesser numerical density of stained puncta and to less intense staining of those remaining. GAD-positive somata were also less intensely stained, whereas their number did not seem to be changed. The changes, apparent at 3 days after the surgery, were restricted to the barrels corresponding to the removed follicles and were maximal at 2–4 weeks. At longer survival times (until 7 months) the immunoreactivity returned to normal, coincident with the regeneration of peripheral nerve fibres in the absence of their follicles. We conclude that GAD-immunoreactivity in the barrel cortex swiftly reacts to modifications of neuronal activity evoked in the periphery.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00247346

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A model system for a fluorometric biosensor using permeabilized Zymomonas mobilis or enzymes with protein confined dinucleotides (1993)

Thordsen, O. ; Lee, S. J. ; Degelau, A. ; [et al.]

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 42 (1993), S. 387-393

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ISSN: 0006-3592

Keywords: biosensor ; glucose ; fructose ; gluconolactone ; sorbitol ; Zymomonas mobilis ; permeabilization ; NADPH ; fluorescence ; bioprocess monitoring ; Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: Using permeabilized Zymomonas mobilis or glucose-fructose oxidoreductase isolated from this microorganism a model system for biosensors with a protein confined NADP(H) cofactor for the determination of glucose, fructose, gluconolactone, and sorbitol was developed. Either permeabilized microorganisms containing the oxidoreductase or the pure enzyme were confined via membrane separation in a small measuring chamber, that was integrated into a flow injection analysis system (FIA). The measuring principle was the monitoring of the NAD(P)H fluorescence, excited at 360 nm and measured at 450 nm. NADP(H), which is confined in the protein complex, was oxidized or reduced during the enzymatic reactions and the changes in the fluorescence intensity were related to the substrate concentration. The sensitivity of the system covered a range from 0.001 to 100 g/L of the analyte depending on substrate and operating conditions. The applicability of this model system for bioprocess monitoring was proved using samples from a Pseudomonas pseudoflava cultivation. © 1993 John Wiley & Sons, Inc.

Additional Material: 13 Ill.