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  • Engineering General  (8)
  • Biochemistry and Biotechnology  (4)
  • 1
    Electronic Resource
    Electronic Resource
    Urea sensor with single poly(propylene) membrane (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 40 (1992), S. 446-449 
    Details
    ISSN: 0006-3592
    Keywords: urea sensor ; plasma reaction ; poly(propylene) membrane ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Urease was immobilized on the plasma-aminated surface of a hyfrophobic poly(propylene) (PP) membrane. This membrane, with urease matrix on one side while maintaining its original hydrophobic property on the other, was used to construct the urea sensor. The new urea sensors had response sensitivities ranged from 19 mV/decade to 30 mV/decade depending on the conditions of the plasma reaction. The enzyme electrode using single membrane gave a shorter response time as compared to the corresponding conventional electrode employing two seperate PP membranes. The sensitivity of the enzyme electrode increased with increasing buffer pH and reached a maximal level (40 mV/decade) at pH 7.6. The response sensitivity of the electrode was not affected by the change of buffer strength. Deamination of the plasma-modified hydrophobic PP membrane did not occur in aqueous environment judging from the stability of the urea electrode up to 12 days of operation. © 1992 John Wiley & Sons. Inc.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260400315
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  • 2
    Electronic Resource
    Electronic Resource
    Pressure-based method for combustion instability analysis (1994)
    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 19 (1994), S. 981-995 
    Details
    ISSN: 0271-2091
    Keywords: Pressure-based method ; All-speed ; Blast wave ; Two-phase flow ; Combustion instability ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: An improved pressure-based method has been applied to predict the two-dimensional instability analysis of liquid-fuelled rocket engines. This method is non-iterative for transient flow calculations and applicable to all-speed flows. Validation cases include the shock-tube problem, the blast flow field and unsteady spraycombusting flows. Computations for the combustion instability analysis were carried out for various combustion parameters such as spray initial conditions and combustor geometries. Unsteady behaviours of the stable and unstable spray flame fields and effects of acoustic oscillations on the fuel droplet vaporization and combustion process are studied in detail. The present numerical model successfully demonstrates the capability of predicting combustion instability as well as fast transient compressible flows at all speeds.
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/fld.1650191103
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  • 3
    Electronic Resource
    Electronic Resource
    A hybrid k-ε turbulence model of recirculating flow (1991)
    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 12 (1991), S. 369-382 
    Details
    ISSN: 0271-2091
    Keywords: Turbulence ; Recirculating flow ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: This investigation deals with the modification of streamline curvature effects in the k-ε turbulence model for the case of recirculating flows. Based upon an idea that the modification of curvature effects in C2 should not be made in regions where the streamline curvature is small, a hybrid k-ε model extended from the modification originally proposed by Srinivasan and Mongia is developed. A satisfactory agreement of model predictions with experimental data reveals that the hybrid k-ε model can perform better simulation of recirculating turbulent flows.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/fld.1650120405
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  • 4
    Electronic Resource
    Electronic Resource
    An efficient pressure-velocity procedure for gas-droplet two-phase flow calculations (1992)
    Chichester : Wiley-Blackwell
    International Journal for Numerical Methods in Fluids 15 (1992), S. 233-245 
    Details
    ISSN: 0271-2091
    Keywords: Two-phase flow ; Pressure-velocity coupling ; Transient flow ; Turbulent dispersion ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: This paper presents a non-iterative numerical technique for computing time-dependent gas-droplet flows. The method is a fully interacting combination of Eulerian fluid and Lagrangian particle calculations. The interaction calculations between the two phases are formulated on a pressure-velocity-coupling procedure based on the operator-splitting technique. This procedure eliminates the global iterations required in the conventional particle-source-in-cell (PSIC) procedure. Turbulent dispersion calculations are treated by a stochastic procedure. Numerical calculations and comparisons with available experimental data as well as efficiency assessments are given for some sprays typical of spray combustion applications.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/fld.1650150207
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  • 5
    Electronic Resource
    Electronic Resource
    Multi-level finite element solution algorithms based on multiplicative and additive correction procedures (1989)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 28 (1989), S. 27-41 
    Details
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: Owing to the failure of the finite element analyst to employ a properly refined computational model, the accuracy of preliminary finite element computations is often low. Thus, it is useful to introduce a computational procedure for improving the results obtained from a preliminary finite element solution. Such a procedure is presented in this paper. In this procedure the solution error components are decomposed into two parts. One of the error components is assumed to have a long period variation. The other component is assumed to have a short period variation. Multiplicative and additive correction procedures are introduced to iteratively eliminate the two error components. The multiplicative and additive correction procedures are implemented using multi-level solution techniques.
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nme.1620280105
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  • 6
    Electronic Resource
    Electronic Resource
    The method of fundamental solutions for non-linear thermal explosions (1995)
    Chichester : Wiley-Blackwell
    Communications in Numerical Methods in Engineering 11 (1995), S. 675-681 
    Details
    ISSN: 1069-8299
    Keywords: blow-up ; method of fundamental solutions ; BEM ; thin plate spline ; Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A numerical method based on the method of fundamental solutions, thin plate spine interpolation and monotone iteration is devised to find the minimal solution of the steady-state blow-up problem. The method of fundamental solutions requires neither domain nor boundary discretization and results in high accuracy and efficiency. For illustration, critical values of the Frank-Kamenetskii parameter are given for different geometrical boundaries in the two-dimensional case.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cnm.1640110806
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  • 7
    Electronic Resource
    Electronic Resource
    Minimal nutritional requirements for immobilized yeast (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 36 (1990), S. 993-1001 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The effect of reduced nutritional levels (particularly nitrogen source) for immobilized K. fragilis type yeast were studied using a trickle flow, “differential” plug flow type reactor with cells immobilized by adsorption onto an absorbant packing matrix. Minimizing nutrient levels in a feed stream to an immobilized cell reactor (ICR) might have the benefits of reducing cell growth and clogging problems in the ICR, reducing feed preparation costs, as well as reducing effluent disposal costs. In this study step changes in test feed medium nutrient compositions were introduced to the ICR, followed by a return to a basal medium. Gas evolution rates were monitored and logged on a continuous basis, and effluent cell density was used as an indicator of cell growth rate of the immobilized cell mass. Startup of the reactor using a YEP medium showed a rapid buildup of cells in the reactor during the initial 110 h operation. The population density then stabilized at 1.6 × 1011 cells/g sponge. A defined medium containing a complex mix of essential nutrients with an inorganic nitrogen source (ammonium sulfate) was able to maintain 90% of the productivity in the ICR as compared to the YEP medium, but proved unable to promote growth of the immobilized cell mass during startup. Experiments on reduced ammonium sulfate in the defined medium, and reduced yeast extract and peptone in YEP medium indicated that stable productivity could be maintained for extended periods (80 h) in the complete absence of any nutrients besides a few salts (potassium phosphate and magnesium sulfate). It was found that productivity rates dropped by 35-65% from maximal values as nitrogenous nutrients were eliminated from the test mediums, while growth rates (as determined by shed cell density from the reactor) dropped by 75-95%. Thus, nutritional deficiencies largely decoupled growth and productivity of the immobilized yeast which suggests productivity is both growth- and non-growth-associated for the immobilized cells. A yeast extract concentration of 0.375 g/L with or without 1 g/L ammonium sulfate was determined to be the minimum level which gave a sustained increase in productivity rates as compared to the nutritionally deficient salt medium. This represents a 94% reduction in complex nitrogenous nutrient levels compared to standard YEP batch medium (3 g/L YE and 3.5 g/L peptone).
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260361004
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  • 8
    Electronic Resource
    Electronic Resource
    The long-term effects of ethanol on immobilized cell reactor performance using K. fragilis (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 36 (1990), S. 975-982 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The effects of ethanol on reactor performance were studied in a small, 5-cm packed height, “differential” type immobilized cell reactor. Lactose utilizing yeast cells, Kluyveromyces fragilis, were absorbed to a porous adsorbant sponge matrix in a gas continuous reactor. Step changes in the feed ethanol concentration to the column (10-130 g/L) were used to test the reactor response over extended periods of time (about 30-50 h per dosage level) followed by a return to basal zero inlet ethanol feed. Effluent cell density and effluent cell viability were measured at intervals. An inhibitory response in ethanol productivity to feed dosage ethanol levels above 20 g/L was detected almost immediately, with a near steady state response noted within 2.5 h of initiating the dosage. Feed ethanol levels above 50 g/L resulted in a subsequent gradual decrease in reactor productivity over time, which was associated with a decrease in the fraction of viable shed cells in the reactor effluent. The reactor response to a step removal of the ethanol inhibition was also monitored. Quick and complete rebounding of the fermentation rate to the original basal rate was noted following dosage concentrations of under 50 g/L ethanol. Recovery rates slowed following ethanol dosage levels above 50 g/L. Viable shed cell density improved overtime during the slow recovery periods. Growth rates (as determined by shed cell density) were more strongly inhibited than productivity. Growth responded more slowly to changes in ethanol environment as growth rates at 30 h fell to about 40% of the rates measured 7.5 h after initiation of a dosage level. It is concluded that ethanol contributions to cell injury and death (and consequent ICR performance degradation) may be more important than ethanol inhibition of productivity rates in the long-term operation of immobilized cell reactors at ethanol concentrations over 50 g/L.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260361002
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  • 9
    Electronic Resource
    Electronic Resource
    Cell growth and death rates as factors in the long-term performance, modeling, and design of immobilized cell reactors (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 36 (1990), S. 983-992 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The viable fraction of immobilized cells in a bioreactor may be critical in predicting long-term or steady-state reactor performance. The assumption of near 100% viable cells in a bioreactor may not be valid for portions of immobilized cell reactors (ICRs) characterized by conditions resulting in appreciable death rates. A mathematical model of an adsorbed cell type ICR is presented in which a steady-state viable cell fraction is predicted, based on the assumptions of no cell accumulation in the reactor and a random loss of cells from the reactor. Data on cell death rates, cell growth rates, and productivity rates as functions of temperature, substrate, and ethanol concentration for the lactose utilizing yeast K. fragillis were incorporated into this model. The steady-state reactor viable cell fraction as predicted by this model is a strong function of both temperature and ethanol concentration. For example, a stable 20% viable fraction of the immobilized cells is predicted in ICR locations experiencing continuous conditions of either 30 g/L ethanol at 40°C, or 95 g/L ethanol at 25°C. Steady-state ICR “plug flow” concentration profiles and column productivities are predicted at three operating temperatures, 20, 30, and 40°C using two different models for ethanol inhibition of productivity. These profiles suggest that the reactor operating temperature should be low if higher outlet ethanol concentrations are desired. Three reactor design strategies are presented to maximize the viable cell fraction and improve long-term ethanol productivity in ICR's: (1) reducing outlet ethanol concentrations, (2) rotating segments of an ICR between high and low ethanol environments, and (3) simultaneous removal of the ethanol produced from the reactor as it is formed.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260361003
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  • 10
    Electronic Resource
    Electronic Resource
    Numerical analysis of the response of reinforced soils to direct shearing: Part 2 (1988)
    New York, NY [u.a.] : Wiley-Blackwell
    International Journal for Numerical and Analytical Methods in Geomechanics 12 (1988), S. 157-171 
    Details
    ISSN: 0363-9061
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Geosciences
    Notes: A soil-reinforcement load transfer model was developed by the authors1 to simulate the response of the reinforced soil material to triaxial compression and direct shearing. This paper presents the application of the proposed model for the numerical analysis of direct shear tests on sand samples reinforced with different types of tension resisting reinforcements. A parametric study is conducted to evaluate the effect of the mechanical characteristics and dilatancy properties of the soil, extensibility (elastic modulus) of the reinforcements, and their inclination with respect to the failure surface on the response of the reinforced soil material to direct shearing. An attempt is made to verify the proposed model by comparing numerical test simulations with experimental results reported by Jewell,2 and Gray and Ohashi.3 Comparisons of predicted and experimental results illustrate that the model can provide adequate simulations of the response of the reinforced soil material to shearing. In particular, it allows an evaluation of the effect of soil dilatancy (or contractancy), and extensibility of the reinforcement on tension forces generated in inclusions during shearing.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nag.1610120204
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