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Effect of hydrogen peroxide on the metabolism of articular chondrocytes (1999)

Asada, S. ; Fukuda, K. ; Oh, M. ; [et al.]

Springer

Inflammation research 48 (1999), S. 399-403

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ISSN: 1420-908X

Keywords: Key words: Annexin V — Chondrocytes — Nitric oxide — Apoptosis — MAP kinase

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Abstract. Objective: To examine the effect of hydrogen peroxide on chondrocyte metabolism.¶Materials and Methods: Bovine articular chondrocytes were used. Proteoglycan (PG) synthesis was measured with [35S] sulfate incorporation. For detection of apoptosis, the TdT-mediated dUTP-biotin nick end labeling (TUNEL) and annexin V assay were used. Extracellular-regulated protein kinase (ERK) activity was measured using a mitogen-activated protein kinase assay system.¶Results: Addition of hydrogen peroxide resulted in the inhibition of PG synthesis, apoptosis, and enhanced ERK activity.¶Conclusion: Hydrogen peroxide plays an important role in regulating the metabolism of chondrocytes.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s000110050478

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Importance of spore mutants for fed-batch and continuous fermentation of Bacillus subtilis (1995)

Oh, M. K. ; Kim, B. G. ; Park, S. H.

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 47 (1995), S. 696-702

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ISSN: 0006-3592

Keywords: Bacillis subtilis ; spore mutant ; fed-batch ; continuous culture ; Chemistry ; Biochemistry and Biotechnology

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology

Notes: To alleviate plasmid instability and to prolong the production phase of subtilisin, integrable plasmid and spore mutants are used. Compared with batch-type shake flask cultures, spore mutants' ability to produce subtilisin can be well pronounced in fed-batch and continuous cultures. Hence, the two culture methods make it possible to identify the peculiar characteristics of the spore mutants unobtainable in batch culture. Spore mutants can enhance subtilisin productivity and prolong subtilisin production time in fed-batch culture as well as enable us to use very low dilution rates (〈0.1 h-1) without losing productivity in continuous culture, thereby improving the conversion yield of the nitrogen source. At 0.05 h-1 the spollG mutant of Bacillus subtilis DB104 (Δnpr Δapr) (Emr) spollG (Bimr):: pMK101 (Cmr) showed a subtilisin yield about ten times higher than that from wild-type DB104 (Δnpr Δapr)::pMK101 (Cmr). © 1995 John Wiley & Sons, Inc.

Additional Material: 2 Ill.