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Digitale Medien

Cell shrinkage stimulates bradykinin-induced cell membrane potential oscillations in NIH 3T3 fibroblasts expressing the ras-oncogene (1993)

Ritter, M. ; Wöll, E. ; Waldegger, S. ; [weitere]

Springer

Pflügers Archiv 423 (1993), S. 221-224

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Details

ISSN: 1432-2013

Schlagwort(e): Cell volume ; Intracellular pH ; ras oncogene ; Calcium oscillations ; Cell membrane potential ; Bradykinin

Quelle: Springer Online Journal Archives 1860-2000

Thema: Medizin

Notizen: Abstract In NIH 3T3 fibroblasts expressing the Ha-ras oncogene (+ras) bradykinin leads to sustained oscillations of cell membrane potential due to oscillations of intracellular Ca2+ with subsequent activation of Ca2+-sensitive K+ channels. In cells not expressing the oncogene (-ras), bradykinin leads only to a single transient hyperpolarization of the cell membrane. The present study has been performed to elucidate the possible interaction of cell volume, intracellular pH and bradykinin-induced oscillations of the cell membrane potential. Bradykinin leads to cell shrinkage and intracellular alkalinization of both +ras cells and −ras cells. Inhibition of Na+/H+ exchanger by HOE 694 abolishes the bradykinin-induced alkalinization but does not significantly interfere with the bradykinin-induced oscillations of cell membrane potential. In contrast, prevention of bradykinin-induced cell shrinkage by simultaneous reduction of extracellular osmolarity blunts the oscillations. Thus, cell shrinkage stimulates bradykinin-induced oscillations of cell membrane potential. On the other hand, cell shrinkage alone does not elicit oscillations unless, in addition, Ca2+ entry is stimulated by ionomycin.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00374398

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Digitale Medien

Exposure of perfused liver to hypotonic conditions modifies cellular nitrogen metabolism (1990)

Häussinger, D. ; Lang, F.

New York, N.Y. : Wiley-Blackwell

Journal of Cellular Biochemistry 43 (1990), S. 355-361

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Details

ISSN: 0730-2312

Schlagwort(e): cell volume ; glutamine ; ammonia ; urea synthesis ; metabolic regulation ; Life and Medical Sciences ; Cell & Developmental Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Chemie und Pharmazie , Medizin

Notizen: Isolated livers were exposed to hypotonic perfusates. As shown previously, this hypotonic challenge leads to initial cell swelling, followed by volume regulatory ion fluxes, largely restoring cell volume within approximately 6 min. However, the hepatocyte is left in an altered metabolic state, which is characterized by marked stimulation of hepatic glutamine uptake and degradation and transient release of glutamate from the liver. Urea formation from glutamine and alanine is stimulated, whereas hepatic ammonia uptake and utilization for urea and glutamine synthesis decreases. These observations reveal a hitherto unrecognized factor modulating hepatic function during intestinal absorption.

Zusätzliches Material: 3 Ill.