ISSN:
1434-4726
Keywords:
Selenium
;
Vitamin A
;
Butylated hydroxytoluene
;
Chemoprevention
;
Human tongue cancer cells in vitro
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract The effects of vitamin A, selenium and butylated hydroxytoluene (BHT) on the growth of a human tongue cancer cell line were examined in monolayer cell cultures. A colony-forming assay showed a 50% reduction in the survival rate of the cell line at a concentration of 2.6 μg/ml selenium, 60 I μg/ml vitamin A and/or 38 μg/ml BHT. Relatively low concentrations of selenium markedly inhibited glucose consumption. Flow cytometric analysis with both fluorescein-isothiocyanate-labelled bromodeoxyuridine monoclonal antibody and propidium iodide demonstrated an increase in S-phase fractions 1 day after the addition of selenium, increased GO/G1 phase fractions in the presence of vitamin A and increased G2M phase fractions when BHT was added. These results suggest that these compounds inhibit DNA synthesis of in vitro human tongue cancer cells by different mechanisms.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02114762
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