ZIB

Hits per page

hits 1 - 2 | 2 hits

Sorting

Electronic Resource

Expression of CD69 on T-cells from HIV-1-infected children and adolescents increases with increasing viral load (1999)

Böhler, T. ; Walcher, J. ; Hölzl-Wenig, G. ; [et al.]

Springer

European journal of pediatrics 158 (1999), S. 638-644

add to mindlist on the mindlist

Details

ISSN: 1432-1076

Keywords: Key words HIV-1 ; T-cells ; CD69 ; Activation ; Children

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Abstract We evaluated the use of a whole-blood assay that measures spontaneous and activation-induced CD69 expression on peripheral blood T-cells in vitro for assessment of T-cell function in HIV-1-infected paediatric patients. Heparinized venous blood from 28 HIV-1 positive children and adolescents and 23 healthy controls was incubated for 4 h with or without 5 μg/ml phytohaemagglutinin (PHA). Thereafter, analysis of CD69 expression on CD4+ and CD8+ T-cells was done by flow cytometry; simultaneously we determined CD4+ T-cell counts and plasma HIV-1 viral load. Neither spontaneous nor PHA-induced CD69 expression differed significantly between HIV-1 positive patients and healthy controls. However, T-cells from HIV-1 positive patients with plasma HIV-1 viral load levels above 70 × 103 copies/ml showed a higher spontaneous CD69 expression than T-cells from patients with lower plasma viral load levels in different stages of the disease. Antiretroviral treatment in four patients reduced spontaneous CD69 expression in CD4+ T-cells and PHA-induced CD69 expression in CD4+ and CD8+ T-cells significantly after 8 weeks of therapy. Conclusion Spontaneous and activation-induced expression of the early (activation) antigen CD69 on peripheral blood T-cells does not distinguish HIV-1 positive patients from HIV-1 negative healthy controls and is not correlated with peripheral blood CD4+ T-cell counts. This test may not be a reliable marker for functional T-cell deficiency during early stages of HIV disease. Increased spontaneous as well as PHA-induced CD69 expression on T-cells from HIV-1-infected children and adolescents in vitro may rather reflect HIV-induced pre-activation of T-cells in vivo.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/s004310051167

Permalink

Library	Location	Call Number	Volume/Issue/Year	Availability

Others were also interested in ...

Paper (German National Licenses)

Fulltext

Electronic Resource

The influence of rubber-matrix interfaces on the crystallization kinetics of isotactic polypropylene blended with ethylene-propylene-diene terpolymer (EPDM) (1993)

Wenig, W. ; Asresahegn, M.

Stamford, Conn. [u.a.] : Wiley-Blackwell

Polymer Engineering and Science 33 (1993), S. 877-888

add to mindlist on the mindlist

Details

ISSN: 0032-3888

Keywords: Chemistry ; Chemical Engineering

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics

Notes: Blends of isotactic polypropylene with amorphous and slightly crystalline ethylene-propylene-diene terpolymer (EPDM), prepared by solution blending, have been investigated by optical microscopy and differential scanning calorimetry. Nucleation and crystallization kinetic parameters, such as nucleation rates, nucleation half times, Avrami-exponents and spherulitic growth rates, have been determined. It has been found that the dispersion of crystalline EPDM in iPP is different from that of amorphous EPDM. Both EPDMs are incorporated into the spherulites, causing a decrease of the maximum growth rate of the iPP spherulites. The surface free energy of the iPP crystals is diminished on adding EPDM to iPP and is accompanied by a higher secondary nucleation rate. From the decrease observed in the Avrami exponent with increasing EPDM concentration in the blend, it has been concluded that nucleation becomes predominantly heterogeneous, as there is a proportional increase in the interfacial area between the two components.

Additional Material: 21 Ill.