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  • 1
    Electronic Resource
    Electronic Resource
    Identification of the DNA damage-responsive elements of the rhp51 + gene, a recA and RAD51 homolog from the fission yeast Schizosaccharomyces pombe (1996)
    Springer
    Molecular genetics and genomics 251 (1996), S. 167-175 
    Details
    ISSN: 1617-4623
    Keywords: Key wordsSchizosaccharomyces pombe ; DNA-damage inducibility ; Damage-responsive element ; Upstream activating sequence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Schizosaccharomyces pombe rhp51 + gene encodes a recombinational repair protein that shares significant sequence identities with the bacterial RecA and the Saccharomyces cerevisiae 1RAD51 protein. Levels of rhp51 + mRNA increase following several types of DNA damage or inhibition of DNA synthesis. An rhp51:: ura4 fusion gene was used to identify the cis-acting promoter elements involved in regulating rhp51 + expression in response to DNA damage. Two elements, designated DRE1 and DRE2 (for damage-responsive element), match a decamer consensus URS (upstream repressing sequence) found in the promoters of many other DNA repair and metabolism genes from S. cerevisiae. However, our results show that DRE1 and DRE2 each function as a UAS (upstream activating sequence) rather than a URS and are also required for DNA-damage inducibility of the gene. A 20-bp fragment located downstream of both DRE1 and DRE2 is responsible for URS function. The DRE1 and DRE2 elements cross-competed for binding to two proteins of 45 and 59 kDa. DNase I footprint analysis suggests that DRE1 and DRE2 bind to the same DNA-binding proteins. These results suggest that the DRE-binding proteins may play an important role in the DNA-damage inducibility of rhp51 + expression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02172915
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Identification of the DNA damage-responsive elements of therhp51 + gene, arecA andRAD51 homolog from the fission yeastSchizosaccharomyces pombe (1996)
    Springer
    Molecular genetics and genomics 251 (1996), S. 167-175 
    Details
    ISSN: 1617-4623
    Keywords: Schizosaccharomyces pombe ; DNA-damage inducibility ; Damage-responsive element ; Upstream activating sequence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract TheSchizosaccharomyces pombe rhp51 + gene encodes a recombinational repair protein that shares significant sequence identities with the bacterial RecA and theSaccharomyces cerevisiae RAD51 protein. Levels ofrhp51 + mRNA increase following several types of DNA damage or inhibition of DNA synthesis. Anrhp51::ura4 fusion gene was used to identify the cis-acting promoter elements involved in regulatingrhp51 + expression in response to DNA damage. Two elements, designated DRE1 and DRE2 (fordamage-responsiveelement), match a decamer consensus URS (upstream repressing sequence) found in the promoters of many other DNA repair and metabolism genes fromS. cerevisiae. However, our results show that DRE1 and DRE2 each function as a UAS (upstream activating sequence) rather than a URS and are also required for DNA-damage inducibility of the gene. A 20-bp fragment located downstream of both DRE1 and DRE2 is responsible for URS function. The DRE1 and DRE2 elements cross-competed for binding to two proteins of 45 and 59 kDa. DNase I footprint analysis suggests that DRE1 and DRE2 bind to the same DNA-binding proteins. These results suggest that the DRE-binding proteins may play an important role in the DNA-damage inducibility ofrhp51 + expression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02172915
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Rheological study on poly-D(-)(3-hydroxybutyrate) and its blends with poly(ethylene oxide) (1995)
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 35 (1995), S. 1636-1642 
    Details
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Aspects of thermal, morphological, and rheological properties of biodegradable poly-D(-)(3-hydeoxybutyrate) (PHB) blended with poly(ethylene oxide) (PEO) have been studied. Thermal properties and morphology of the blends were examined by scanning electron microscopy and differential scanning calorimetry, respectively. A rotational theometer with parallel plate geometry was also adopted to investigate the rheological properties of these blends. In addition, dynamic ciscoelasticity was measured by a Rheovibron as functions of time and temperature. From these measurements, PHB and PEO were observed to be miscible in the melt state. In the case of the blend systen 80/20 PHB/PEO by weight, the vacant domains of the PHB were filled with PEO particles, and this morphological state enhanced the rheological properties. Furthermore, PHB and its blends were found to have high crystallinities, but to have unstable thermal behavior about Tm.
    Additional Material: 13 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pen.760352010
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Error in the propagation of error formula (1980)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 26 (1980), S. 168-170 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690260132
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Letters to the editor (1981)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 27 (1981), S. 527-528 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690270332
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    Paper (German National Licenses)
    Fulltext
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