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  • DNA markers  (2)
  • 2320.Lv  (1)
  • 1
    Electronic Resource
    Electronic Resource
    The deformedN=60 nucleus 41 101 Nb (1991)
    Springer
    The European physical journal 340 (1991), S. 5-16 
    Details
    ISSN: 1434-601X
    Keywords: 23.20.Ck ; 2320.Lv ; 27.60.+j
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The β− decay of101Zr has been investigated at the fission-product separators JOSEF and LOHENGRIN. The half-life of101Zr has been determined to 2.5(1) s and a level scheme for101Nb has been established fromγ ray singles as well as X/3-γ and γ—γ coincidence measurements. Conversion coefficients for transitions in101Nb and level half-lives between 10 ps and 2 ns have been determined. Three rotational bands are identified among the low-lying levels with band heads at 0 keV, 206 keV and 208 keV. The bands are probably based on the Nilsson configurations [422 5/2+], [301 3/2−] and [303 5/2−], respectively. The deformation has been determined to βq=0.40(4) and 0.41(8) for the ground state band and the band based on the 206 keV level from the half-lives of the first and second excited members of these bands. This shows that the rapid onset of deformation at N=60 which is typical for the A=100 region of neutron-rich nuclei, takes also place in the Nb isotopes. Nilsson model calculations describe the experimental data well, especially the several determined transition probabilities including those for E1 transitions from the 206 and 208 keV band heads to the ground state.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01284474
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  • 2
    Electronic Resource
    Electronic Resource
    Association of a DNA marker with Hessian fly resistance gene H9 in wheat (1994)
    Springer
    Theoretical and applied genetics 89 (1994), S. 964-968 
    Details
    ISSN: 1432-2242
    Keywords: RAPD ; Wheat ; Hessian fly ; DNA markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Hessian fly [Mayetiola destructor (Say)] is a major pest of wheat (Triticum aestivum L.) and genetic resistance has been used effectively over the past 30 years to protect wheat against serious damage by the fly. To-date, 25 Hessian fly resistance genes, designated H1 to H25, have been identified in wheat. With near-isogenic wheat lines differing for the presence of an individual Hessian fly resistance gene, in conjunction with random amplified polymorphic DNA (RAPD) analysis and denaturing gradient-gel electrophoresis (DGGE), we have identified a DNA marker associated with the H9 resistance gene. The H9 gene confers resistance against biotype L of the Hessian fly, the most virulent biotype. The RAPD marker cosegregates with resistance in a segregating F2 population, remains associated with H9 resistance in a number of different T. aestivum and T. durum L. genetic backgrounds, and is readily detected by either DGGE or DNA gel-blot hybridization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224525
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  • 3
    Electronic Resource
    Electronic Resource
    Detection of DNA sequence polymorphisms among wheat varieties (1992)
    Springer
    Theoretical and applied genetics 84 (1992), S. 573-578 
    Details
    ISSN: 1432-2242
    Keywords: Common wheat ; RAPD ; DGGE ; DNA polymorphism ; DNA markers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A DNA marker detection strategy that allows the rapid, efficient resolution of high levels of polymorphism among closely related lines of common wheat (Triticum aestivum) has been developed to circumvent the apparent lack of restriction fragment length polymorphism in many important self-pollinated crop species. The technique of randomly amplified polymorphic DNA (RAPD) was combined with a denaturing gradient gel electrophoresis system (DGGE) to explore DNA sequence polymorphisms among different genotypes of wheat. Of the 65 primer combinations used for the polymerase chain reaction (PCR) amplifications, over 38% of them produced readily detectable and reproducible DNA polymorphisms between a spring wheat line, SO852, and a winter wheat variety, ‘Clark’. A high level of polymorphism was observed among a number of commercial varieties and breeding lines of wheat. This procedure was also used to detect polymorphisms in a recombinant inbred population to test the feasibility of its application in genome mapping. This DNA polymorphism detection system provides an opportunity for pedigree analysis and fingerprinting of developed wheat lines as well as construction of a high density genetic map of wheat. Without the need for 32P and sophisticated DNA extraction procedures, this approach should make it feasible to utilize marker-based selection in a plant breeding program.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224154
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