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  • 1
    Electronic Resource
    Electronic Resource
    The suppressive effect of tape-stripping treatment of guinea-pig skin on the induction of contact sensitivity by intradermal injection of haptenated epidermal cells (1985)
    Springer
    Archives of dermatological research 278 (1985), S. 102-106 
    Details
    ISSN: 1432-069X
    Keywords: Tape stripping ; Contact sensitivity ; DNCB ; Dinitrophenylated epidermal cell ; Immunological suppression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Contact sensitivity (CS) to 2,4-dinitrochlorobenzene (DNCB) was produced in inbred JY1-strain guinea pigs by the intradermal injection of epidermal cells (ECs) prepared from DNCB-painted skin (DNP-ECs). When the site of DNP-EC-induced CS was pretreated by tape stripping, the rate and intensity of the challenge reactions to DNCB were diminished. The ability of DNP-ECs to induce CS returned to normal when normal peritoneal macrophages together with DNP-ECs were administered into the stripped skin. Normal ECs had a similar effect. Using either anti-Ia antiserum and complement or allogeneic ECs (strains 2 and 13), Ia-positive cells among the ECs (presumably Langerhans cells) were found to be essential for the recovery of CS. Tape-stripping treatment also resulted in the development of immunological tolerance, as assessed by subsequent painting with a sensitizing dose of DNCB. These findings suggest that the immunological function of the mononuclear-phagocyte system in the dermis may be impaired when the epidermal surface is markedly disturbed by tape-stripping treatment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00409215
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    The distribution of 2,4-dinitrophenyl groups in guinea pigs following feeding of 2,4-dinitrochlorobenzene (1985)
    Springer
    Archives of dermatological research 278 (1985), S. 97-101 
    Details
    ISSN: 1432-069X
    Keywords: Contact sensitivity ; Tolerance ; Feeding ; DNCB ; Antigen distribution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary An immunofluorescent technique using anti-DNP antibody was employed to investigate the distribution of 2,4-dinitrophenyl (DNP) groups in various tissues following the feeding of 125 mg/kg 2,4-dinitrochlorobenzene (DNCB)-ethanol to guinea pigs that had been starved for 1 day. DNP groups were detected in the areas corresponding to the cytoplasm and the cell membrane of the epithelium in the upper gastrointestinal tract as well as on the cells of mesenteric lymph nodes, Payer's patches, the spleen, and peripheral blood. These results are discussed in relation to the mechanism of tolerance induction produced by the feeding of haptens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00409214
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Histological study on the fate of haptenated epidermal cells injected intradermally in guinea pigs (1987)
    Springer
    Archives of dermatological research 279 (1987), S. S111 
    Details
    ISSN: 1432-069X
    Keywords: 2,4-Dinitrochlorobenzene ; 2,4-Dinitrophenylated epidermal cells ; Contact sensitivity ; Spontaneous flare ; Delayed-type skin reaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The percutaneous administration of in vitro haptenated epidermal cells (EC) has become established as a procedure to produce contact sensitivity (CS) in experimental animals for routine use. The cells have also been found to elicit a significant delayed-type skin reaction by intradermal test in the animals sensitized by painting the skin with the hapten. The fate of 2,4-dinitrophenylated (DNP) isogeneic epidermal cell suspensions (EC) injected intradermally was investigated histologically in intact or 2,4-dinitrochlorobenzene (DNCB)-sensitized strain 13 guinea pigs to study the role of the cells in CS. DNP-EC were found to proliferate actively in the dermis and formed EC nests with central keratinization and then elicited inflammatory reaction associated with necrosis of the epidermal structures 7 days after injection in the intact animals. DNP-EC injected intradermally into the animals which had received and reacted against DNCB underwent a suppression of EC proliferation. These findings are discussed in relation to the role of the haptenated EC in CS.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00585933
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    Paper (German National Licenses)
    Fulltext
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