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  • 1
    Digitale Medien
    Digitale Medien
    A variant of finite-dimensional Tikhonov regularization with a-posteriori parameter choice (1988)
    Springer
    Computing 40 (1988), S. 91-109 
    Details
    ISSN: 1436-5057
    Schlagwort(e): 65R20 ; 45L10 ; Integral equations ; Tikhonov regularization ; parameter selection method
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Informatik
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung In dieser Arbeit betrachten wir eine besondere Variante endlich-dimensionaler Tikhonov-Regularisierung schlechtgestellter Operatorgleichungen. Konvergenzraten werden nachgewiesen und eine a-posteriori Parameterwahl wird hergeleitet, die optimale Konvergenzraten bezüglich des Datenfehlers und der endlich-dimensionalen Approximation liefert, ohne Information über die exakte Lösung zu benötigen. Schließlich präsentieren wir auch einige numerische Beispiele, bei denen lineare Splinefunktionen verwendet werden, die die theoretischen Ergebnisse bestätigen.
    Notizen: Abstract In this paper we consider a particular variant of finite-dimensional Tikhonov regularization for ill-posed operator equations. Convergence rates are established and an a-posteriori parameter choice method is derived that leads to optimal convergence rates with respect to data errors and with respect to the finite-dimensional subspace, without using any information about the exact solution. Finally, using linear splines we present several numerical examples that confirm the theoretical results.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02247939
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  • 2
    Digitale Medien
    Digitale Medien
    Multilevel algorithms for ill-posed problems (1992)
    Springer
    Numerische Mathematik 61 (1992), S. 311-334 
    Details
    ISSN: 0945-3245
    Schlagwort(e): 65R20
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Mathematik
    Notizen: Summary In this paper new multilevel algorithms are proposed for the numerical solution of first kind operator equations. Convergence estimates are established for multilevel algorithms applied to Tikhonov type regularization methods. Our theory relates the convergence rate of these algorithms to the minimal eigenvalue of the discrete version of the operator and the regularization parameter. The algorithms and analysis are presented in an abstract setting that can be applied to first kind integral equations.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01385512
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  • 3
    Digitale Medien
    Digitale Medien
    Introduction of resistance to kanamycin into the protoplasts from a pantothenate-requiring auxotrophic cell line of Datura innoxia P. Mill. via direct gene transfer
    Amsterdam : Elsevier
    Plant Science 70 (1990), S. 105-114 
    Details
    ISSN: 0168-9452
    Schlagwort(e): Datura ; auxotrophy ; direct gene transfer ; kanamycin-resistance ; protoplasts ; universal hybridizer
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie
    Materialart: Digitale Medien
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9452(90)90038-P
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  • 4
    Digitale Medien
    Digitale Medien
    Somatic hybrids derived from fusion of a universal hybridizer and a wild type line of Datura
    Amsterdam : Elsevier
    Plant Science 79 (1991), S. 111-118 
    Details
    ISSN: 0168-9452
    Schlagwort(e): Datura ; auxotrophy ; cell culture ; kanamycin resistance ; somatic hybrid ; universal hybridizer
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie
    Materialart: Digitale Medien
    URL: http://linkinghub.elsevier.com/retrieve/pii/0168-9452(91)90077-L
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  • 5
    Digitale Medien
    Digitale Medien
    Transplantation of isolated nuclei into plant protoplasts (1986)
    Springer
    Planta 168 (1986), S. 29-35 
    Details
    ISSN: 1432-2048
    Schlagwort(e): Auxotrophy ; Datura ; DNA transfer ; Nuclear transplantation ; Protoplast ; Prototrophy ; Vicia (nuclear transplantation)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The uptake of isolated nuclei from Vicia hajastana Grossh. cells into protoplasts of an auxotrophic cell line of Datura innoxia P. Mill. was induced under the influence of polyethylene glycol and Ca2+ at pH 6.8. The frequency of nuclear uptake varied from 0.8 to 2.3% and that of the recovery of prototrophic clones from 10-5 to 6·10-4. The prototrophic nuclear fusion products following nuclear uptake could be rescued by initial culture of the protoplasts in non-selective conditions and by the subsequent use of feeder cell layers to support the growth of surviving colonies on a selective medium. The presence of Vicia genomic DNA in some prototrophic clones was confirmed by dot-blot hybridization using Datura and Vicia DNA probes. In certain transformed clones, the recovery of prototrophy was accompanied by the restoration of morphogenetic potential. Welldeveloped shoots typical of wild-type Datura could be regenerated employing an appropriate regeneration medium.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00407005
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  • 6
    Digitale Medien
    Digitale Medien
    A valine-resistant mutant ofArabidopsis thaliana displays an acetolactate synthase with altered feedback control (1994)
    Springer
    Planta 192 (1994), S. 249-255 
    Details
    ISSN: 1432-2048
    Schlagwort(e): Acetolactate synthase ; Amino acid (branched chain) ; Arabidopsis ; Feedback regulation ; Mutant ; Valine resistance
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A valine-resistant mutant line, VAL-2, ofArabidopsis thaliana (L.) Heynh. was identified by screening M 2 populations of ethylmethane-sulfonate-mutagenized seeds. The resistance was found to be due to a single, dominant, nuclear gene mutation. Assay of acetolactate synthase (ALS) indicated that the valine resistance in this mutant is caused by decreased sensitivity of ALS to the branched-chain amino acids, valine, leucine andisoleucine. A two fold decrease in apparentK m value for pyruvate of the mutant ALS enzyme was detected compared with that of the wild type. The sensitivity of the ALS enzyme to sulfonylurea, imidazolinone and triazolopyrimidine herbicides was not altered in the mutant. At the plant growth level the mutant was also resistant to valine plus leucine, but was sensitive to leucine orisoleucine alone. The mutant gene,var1, maps, or is very closely linked, toCSR1, the gene encoding acetolactate synthase inArabidopsis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01089041
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  • 7
    Digitale Medien
    Digitale Medien
    A valine-resistant mutant of Arabidopsis thaliana displays an acetolactate synthase with altered feedback control (1994)
    Springer
    Planta 192 (1994), S. 249-255 
    Details
    ISSN: 1432-2048
    Schlagwort(e): Acetolactate synthase ; Amino acid (branched chain) ; Arabidopsis ; Feedback regulation ; Mutant ; Valine resistance
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A valine-resistant mutant line, VAL-2, of Arabidopsis thaliana (L.) Heynh. was identified by screening M 2 populations of ethylmethane-sulfonate-mutagenized seeds. The resistance was found to be due to a single, dominant, nuclear gene mutation. Assay of acetolactate synthase (ALS) indicated that the valine resistance in this mutant is caused by decreased sensitivity of ALS to the branched-chain amino acids, valine, leucine and isoleucine. A two fold decrease in apparent K m value for pyruvate of the mutant ALS enzyme was detected compared with that of the wild type. The sensitivity of the ALS enzyme to sulfonylurea, imidazolinone and triazolopyrimidine herbicides was not altered in the mutant. At the plant growth level the mutant was also resistant to valine plus leucine, but was sensitive to leucine or isoleucine alone. The mutant gene, var1, maps, or is very closely linked, to CSR1, the gene encoding acetolactate synthase in Arabidopsis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00194459
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  • 8
    Digitale Medien
    Digitale Medien
    A transient duplication of the acetolactate synthase gene in a cell culture of Datura innoxia (1987)
    Springer
    Theoretical and applied genetics 74 (1987), S. 417-422 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Gene amplification ; Datura ; Sulfonylurea resistance ; Plant cell culture
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary A 2.0 kb fragment of the yeast ILV2 gene, which codes for the target enzyme acetolactate synthase (ALS) of the herbicide chlorsulfuron, was shown to hybridize to the nuclear DNA of a haploid cell culture of Datura innoxia P. Mill. Nuclear DNA of a chlorsulfuron resistant line of D. innoxia, CSR6, gave a prominent 2.65 kb band when cleaved by either EcoRI or HindIII. The 2.65 kb band has been shown to hybridize with the yeast ILV2 probe. A herbicide resistant line descended from CSR6 by continuous culture resulted in the loss of the 2.65 kb restriction fragment. These observations suggest that CSR6 resulted from a large tandem duplication of the ALS gene and that a point mutation for herbicide resistance in an ALS gene repeat unit of the duplication was selected during subsequent growth of the resistant line.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00289814
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