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  • Decalcification  (1)
  • Key words KAI1, Ovarian tumors  (1)
  • Key words: Dual-step pressurization, Film condensation, Retrograde fluid  (1)
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  • 1
    Electronic Resource
    Electronic Resource
    Clinical implications of mRNA expression of KAI1 in ovarian cancer with or without metastasis (1999)
    Springer
    International journal of clinical oncology 4 (1999), S. 343-347 
    Details
    ISSN: 1437-7772
    Keywords: Key words KAI1, Ovarian tumors ; PCR ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Background. KAI1 is a potential metastasis suppressor gene for prostate cancer. Decreased expression of KAI1 mRNA has been shown to be associated with the formation of metastasis and the progression of prostate, lung, breast, pancreatic, and bladder cancer. It has also been reported, however, that KAI1 expression is unchanged in metastatic and nonmetastatic esophageal and gastric cancer. We performed the present study to investigate the function of KAI1 in the progression and/or metastasis of ovarian cancer. Methods. We investigated the mRNA expression levels of the KAI1 gene, using quantitative polymerase chain reaction (PCR), in 29 ovarian tumors (1 adenoma, 2 low- malignant potential tumors, 9 adenocarcinomas without metastasis, and 17 adenocarcinomas with metastasis), seven ovarian cancer cell lines, and two normal ovaries. Using a thermal cycler, we found that the KAI1 gene was amplified in parallel with an internal control gene, β-Actin. The relative expression ratio (KAI1/β-Actin) as measured by densitometry was used to evaluate gene expression. Immunohistochemical localization of the KAI1 protein in ovarian cancer tissues was confirmed by the avidin-biotin peroxidase complex (ABC) method. Results. The mRNA expression levels of KAI1 were consistent in normal ovary, ovarian tumor samples, and ovarian cancer cell lines. No statistically significant difference in the KAI1 mRNA expression level was found in ovarian cancer samples with or without metastasis. Immunohistochemistry revealed that the KAI1 protein was expressed in the cell membranes of ovarian cancer cells. Conclusions. Our results suggest that reductions in KAI1 mRNA expression are not involved in either the progression or metastasis of ovarian carcinomas.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s101470050082
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Ultrastructural aspects of human dentine decalcified with chromium sulphate (1969)
    Springer
    Calcified tissue international 4 (1969), S. 305-313 
    Details
    ISSN: 1432-0827
    Keywords: Decalcification ; Chromium Sulphate ; Ultrastructure ; Dentine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé Une méthode nouvelle de décalcification histologique utilisant des solutions aqueuses de sulfate de chrome basique (III) est appliquée à des coupes fines de dentine humaine adulte. L'observation en microscopie électronique montre une bonne conservation structurale dans les régions extra-cellulaires. L'aspect de la matrice péricanaliculaire est particulièrement étudié. On y reconnait plusieurs couches bien individualisées. Une bonne concordance est notée entre les résultats obtenus par microscopie optique et électronique, permettant d'établir une hypothèse nouvelle sur la formation de la dentine péricanaliculaire.
    Abstract: Zusammenfassung Eine kürzlich entwickelte Methode zur histologischen Entkalkung mittels wäßriger Lösungen von basischem Chrom III-Sulfat wird an dünnen Schliffen von menschlichem Dentin Erwachsener angewandt. Darauffolgende Untersuchungen am Elektronenmikroskop zeigten eine sehr gute Wiedergabe der morphologischen Einzelheiten in den Extrazellulär-Regionen. Besonders beachtet wurde das Aussehen der peritubulären Matrix, innerhalb welcher gut differenzierte Schichten erkannt werden konnten. Eine wesentliche Übereinstimmung bestand zwischen Elektronenmikroskopie und kürzlich durchgeführten Untersuchungen an derselben Matric unter dem Mikroskop. Diese Methode ermöglicht es, eine neue Hypothese über die Bildung des peritubulären Dentins aufzustellen.
    Notes: Abstract A recently-developed method for histological decalcification using aqueous solutions of basic chromium (III) sulphate has been applied to thin sections of adult human dentine. Subsequent studies in the electron microscope show a very good retention of morphological detail in the extracellular regions. Special attention was given to the appearance of the peritubular matrix within which well-differentiated layers were recognised. There was substantial agreement between electron microscopy and recent studies of the same matric under the light microscope, allowing a novel hypothesis for the formation of the peritubular dentine.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02279133
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Vapor condensation behind a shock wave propagating through a large molecular-mass medium (1996)
    Springer
    Shock waves 5 (1996), S. 287-292 
    Details
    ISSN: 1432-2153
    Keywords: Key words: Dual-step pressurization, Film condensation, Retrograde fluid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics , Technology
    Notes: Abstract. Measurements of film condensation were made behind the incident shock wave propagating through a vapor-liquid two-phase medium. Major objective of the study is to identify condensing heat transfer rates of the vapor to the shock-tube side wall as well as to learn the condensing main flow field. Ethanol and E-10 (a heavy liquid named Afluid by the manufacturer) were extensively used as working fluid. Steady accumulation of the condensing vapor was confirmed on the wall surface, as similarly seen in the end-wall experiment conducted elsewhere. A most significant result is that "dual-step" shock pressurization was observed in E-10. The first pressure rise is a normal one created by an incident shock front, whereas the second pressure rise is taken place by some large disturbance in the main flow. The reason for this is not certain yet, but is speculated to be a long relaxation time or inefficient compressibility of the fluid. The visualized shock front and its vicinity of E-10 is completely different from those of normal gases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00003873
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    Paper (German National Licenses)
    Fulltext
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