ISSN:
1432-0878
Schlagwort(e):
Germinal center reaction
;
Dendritic reticulum cell
;
Rabbit spleen
;
Enzyme histochemistry
;
Electron microscopy
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
,
Medizin
Notizen:
Summary To obtain more information concerning the origin of dendritic reticulum cells, the development of germinal centers in the spleens of rabbits was investigated by conventional light microscopy, enzyme histochemistry, and electron microscopy. Washed sheep erythrocytes were used as antigen. Splenic tissue was examined on the 13th, 18th, 21st, 27th and 48th day after antigen administration. Electron microscopic investigations revealed transitional forms between typical fibroblastic reticulum cells, which formed the framework of the entire splenic white pulp, and typical dendritic reticulum cells. During this transformation, the enzyme histochemical pattern of alkaline phosphatase disappeared and a positive alpha-naphthyl acetate esterase reaction appeared in the transformed cells. On the basis of these findings, it is highly likely that dendritic reticulum cells develop through transformation of fibroblastic reticulum cells during the development of germinal centers in rabbit spleens. The characteristic folding of the surface membrane of dendritic reticulum cells is probably caused by the conspicuous increase in size of the Golgi apparatus, the detachment of vesicles, and the uptake of such vesicles by the cell membrane observed electron microscopically during the cellular transformation. Receptors that are of significance in antigen trapping might reach the cell surface in this manner, i.e., with the Golgi vesicles.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00237632
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