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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Clinical oral investigations 1 (1997), S. 125-130 
    ISSN: 1436-3771
    Keywords: Key words Fluoride ; Dentin ; Dentinal fluid flow
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The aim of the present study was to compare fluoride uptake of dentin with and without simulating dentinal fluid flow. Eighty-five dentinal discs were prepared from retained human molars. Seventeen discs were used to assess baseline fluoride content (controls). Sixty-eight discs were evenly distributed among two experimental groups and were fixed for 24 h in a two-chamber device allowing for simulating dentinal fluid flow. Thirty-four samples were treated with dentifrice slurry containing a low fluoride concentration (Elmex, dilution 1:5); the remaining discs were treated with a solution of a high fluoride concentration (Elmex Fluid). For the perfused group, each of the 17 discs of the high and low concentration fluoride groups were constantly perfused during the experiment. The remaining fluoridated specimens were not perfused. All specimens were assayed for KOH-soluble fluoride and structurally bound fluoride. Structurally bound fluoride was determined in three successive layers of 20 µm each. Uptake of KOH-soluble fluoride was significantly higher in the specimens fluoridated with Elmex Fluid compared to the samples treated with the dentifrice slurry. The uptake of KOH-soluble fluoride was not influenced by perfusion of the dentinal discs. However, perfusion resulted in lower acquisition of structurally bound fluoride in the samples treated with the dentifrice slurry. After application of Elmex Fluid, no significant difference was found in the amount of structurally bound fluoride between the perfused specimens and non-perfused samples. It is suggested that further studies on fluoride uptake in dentin should use a model simulating outward dentinal fluid flow.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1433-8580
    Keywords: Edema, traumatic ; Rat paw ; Ödem, traumatisches ; Rattenpfote
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Berichtet wird über ein Versuchsmodell, das es zuverlässig ermöglicht, am Beispiel des Rattenpfotentestes ein posttraumatisches Ödem mit hoher Volumenausbeute und sicherer Reproduzierbarkeit unter größtmöglicher Gewebeschonung zu erzeugen. Das Ödem wird durch Quetschen der Rattenhinterpfote unter standardisierten Bedingungen in einer Wringe mit gegenläufigen Holzwalzen induziert und volumetrisch gemessen. Die Ergebnisse werden diskutiert und denen anderer Versuchsmodelle gegenübergestellt.
    Notes: Summary A standardized reproducible technique is reported for inducing traumatic edema in the rat paw showing considerable swelling volume and low tissue damage. The edema is measured volumetrically subsequent to squashing the rat paw under standardized conditions in a wringing-machine with two wooden rollers. The technique is compared with other methods.
    Type of Medium: Electronic Resource
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