ISSN:
1432-0738
Keywords:
Key words Artificial antigen
;
Competitive inhibition enzyme immunoassay
;
Sarin
;
Detection
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Two artificial antigens, N α N ɛ-di(O, O-diisopropyl) phosphoryl L-lysine (DIP)- bovine serum albumin (BSA) conjugate (DIP-BSA) and DIP-KLH (keyhole limpet hemocyanin), were synthesized. Antibodies against sarin (O-isopropyl methylphosphonofluoridate) were obtained after immunization of rabbits with DIP-KLH conjugate. A competitive inhibition enzyme immunoassay (CIEIA) was developed to detect the organophosphorus nerve agent sarin. The antibody solutions could be inhibited by sarin as low as 10-6 mol/l, and the standard curve was linear over 3 orders of magnitude. The coefficients of intraassay and interassay variation of this method were 5.4–6.2% (n=11) and 8.0–9.5% (n=6) at a sarin concentration range of 10-3–10-6 mol/l, respectively. The recovery of sarin in water samples at the concentration of 5×10-5 mol/l was in the range of 96.8–102.5%. The specificity of the antiserum was assessed by comparing the inhibition induced by sarin with soman, Vx, isopropyl alcohol and isopropyl methyl phosphonic acid. The results showed that less than 5 mmol/l soman, 2 mmol/l Vx, 16 mmol/l isopropyl alcohol and 8 mmol/l isopropyl methyl phosphonic acid did not influence the determination of sarin in water samples.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002040050226
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