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  • 1995-1999  (3)
  • Dunaliella viridis  (2)
  • Carbohydrates  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Optimized nitrate reductase assay predicts the rate of nitrate utilization in the halotolerant microalga Dunaliella viridis (1997)
    Springer
    Journal of applied phycology 9 (1997), S. 99-106 
    Details
    ISSN: 1573-5176
    Keywords: Nitrate reductase ; in situ enzymatic activity ; Dunaliella viridis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An in situ method for measuring nitrate reductase (NR) activity in Dunaliella viridis was optimized in terms of incubation time, concentration of KNO3, permeabilisers (1-propanol and toluene), pH, salinity, and reducing power (glucose and NADH). NR activity was measured by following nitrite production and was best assayed with 50 mM KNO3, 1.2 mM NADH, 5% 1-propanol (v/v), at pH 8.5. The estimated half-saturation constant (Ks) for KNO3 was 5 mM. Glucose had no effect as external reducing power source, and NADH concentrations 〉1.2 mM inhibited NR activity. Nitrite production was linear up to 20 min; longer incubation did not lead to higher nitrate reduction. The use of the optimized assay predicted the rate of NO 3 − removal from the external medium by D. viridis with high degree of precision.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007978512458
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Effect of turbulence and inorganic carbon supply on growth ofDunaliella viridis Teodoresco (1995)
    Springer
    International journal of salt lake research 4 (1995), S. 223-232 
    Details
    ISSN: 1573-8590
    Keywords: Dunaliella viridis ; subsidiary energy ; turbulence ; carbon supply ; photosynthetic efficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geography
    Notes: Abstract Turbulence is known to enhance growth in microalgae. We studied the possible effects of the extra inorganic carbon supplied to the cultures when air bubbling is the source of turbulence. A set of experiments was carried out to separate the effects of the subsidiary action of agitation on growth from those due to possible carbon enrichment from air CO2 when air bubbling was the source of mixing. It was found that air bubbling does not induce an increase of the concentration of inorganic carbon in HCO 3 − -enriched growth media, and that no differences appear between air-bubbled and CO2-free air-bubbled cultures. On the other hand, photosynthetic O2 was accumulated in the medium in non-mixed cultures, which showed higher respiration and lower photosynthetic rates, probably due to photoinhibition induced by oversaturation of O2. Growth rate, maximal cell density and photosynthetic efficiency were higher in aerated cultures than in non-aerated ones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02001492
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Cytotoxic Triterpene Glycosides from Far-Eastern Sea Cucumbers Belonging to the Genus Cucumaria (1997)
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 1997 (1997), S. 2351-2356 
    Details
    ISSN: 0947-3440
    Keywords: Carbohydrates ; Cucumaria japonica ; Cucumaria miniata ; Sea cucumbers ; Cucumariosides ; Triterpene glycosides ; Antitumor activity ; Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: New cytotoxic triterpene oligoglycoside disulfates, cucumariosides A3 (1) and A6-2 (2), have been isolated from the sea cucumber Cucumaria japonica. The trisulfated cucumarioside A7-3 (4) was found to be the major component in the glycoside fraction of Cucumaria miniata. The structures were assigned on the basis of extensive spectral data (NMR and MS), selective solvolysis of desulfated derivative 3 and other chemical evidence. The presence or absence of a carbonyl group at C-16 of the aglycone and of sulfate groups at C-6 of the glucose and 3-O-methylglucose units are interpreted in terms of the functional evolution of sea cucumbers in order to improve their defensive system. Both (1) and (2) are cytotoxic in vitro at IC50 = 1 μg/ml against a selection of five human and mice tumoral cell lines.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jlac.199719971125
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    Paper (German National Licenses)
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