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  • 1
    Electronic Resource
    Electronic Resource
    Serological characterization of fluorescent Pseudomonas strains cross-reacting with antibodies against Erwinia chrysanthemi (1993)
    Springer
    European journal of plant pathology 99 (1993), S. 51-60 
    Details
    ISSN: 1573-8469
    Keywords: ELISA ; immunofluorescence cell-staining ; Ouchterlony double diffusion ; SDS-PAGE ; Western blotting ; lipopolysaccharides ; fatty acid analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Sixteen bacterial strains, cross-reacting with antibodies againstErwinia chrysanthemi (Ech), were isolated from potato peel extracts, ditch water, and the rhizosphere of wheat, onion, sugar beet and chicory using the immunofluorescence colony-staining procedure. Based on fatty acid profiles, isolates were classified as belonging to thePseudomonas fluorescens group. These strains, together with two previously isolated cross-reactingP. fluorescens strains, crossreacted with polyclonal antibodies against Ech in immunofluorescence cell-staining, Ouchterlony double diffusion, and ELISA. Seventeen strains also reacted strongly with monoclonal antibodies against the lipopolysaccharides (LPS) of Ech in ELISA. Cell envelopes (CE) and proteinase-K-treated CE (mainly LPS) of cross-reacting bacteria were further characterized with SDS-PAGE and Western blotting. Based on CE protein and LPS patterns, the cross-reacting bacteria were classified into two groups, each existing of two subgroups. Both CE and proteinase-K-resistant antigens strongly cross-reacted on immunoblots with antisera against a wild type strain of Ech. With an antiserum against a LPS O-chain lacking mutant of Ech only protein bands but no proteinase-K-resistant antigens were detected on immunoblots. These data suggest that in all cases the highly antigenic LPS O-chain is responsible for the cross-reactions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01998473
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Evaluation of a PCR kit for the detection ofErwinia carotovora subsp.atroseptica on potato tubers (1998)
    Springer
    Potato research 41 (1998), S. 163-173 
    Details
    ISSN: 1871-4528
    Keywords: blackleg ; ELISA ; DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A PCR-based kit, ProbeliaTM, for the detection ofErwinia carotovora subsp.atroseptica (Eca) on potatoes was evaluated at five laboratories in four countries. The kit is based on DNA-specific PCR amplification followed by detection of amplicons by hybridization to a peroxidase-labelled DNA probe in a microplate. Specificity of the PCR primers for Eca, regardless of serogroups, was confirmed by testing against 246 bacterial, fungal and plant species. Detection limits of the assay varied little between six Eca strains in pure cultures (1.3×102 to 1.5×103 cells ml−1). When Eca-free tuber peel extract from four cultivars was inoculated with known numbers of 15 Eca strains, detection limits were more variable (1.0×101 to 6.2×103 cells ml−1 peel extract), attributed probably to inconsistency in the recovery of DNA during extraction. When the PCR assay was compared with three current commercial Eca detection methods, using naturally contaminated tubers, results matched most closely those from viable counts on a selective medium, the most sensitive method (88%), followed by enrichment ELISA (72%) and last ELISA (30%), the least sensitive method.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02358439
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    Paper (German National Licenses)
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