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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    European archives of oto-rhino-laryngology and head & neck 252 (1995), S. 249-254 
    ISSN: 1434-4726
    Keywords: Middle ear ; Glycoconjugate ; Wheat germ agglutinin ; Electron microscopy ; Guinea pig
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract To evaluate the protective function of the mucous blanket (MB) against lectin substances, we examined at the ultrastructural level whether intraluminal colloidal gold-labelled wheat germ agglutinin (WGA) could enter the MB-covered epithelial cell surface of the guinea pig tubotympanic mucosa. Post-embedding staining with WGA/gold on thin tissue sections was done in parallel for comparison. The cell surface glycoconjugate of the eustachian tubal and transitional epithelium had a typical bilayered structure: the outer MB and the microvilli-associated glycocalyx (MAG), which were interposed by the interciliary fluid zone. In squamous epithelium of the distal middle ear, the MB adhered to the MAG, thereby forming a monolayered coat of glycoconjugates at the cell surface. In the pre-embedding staining, WGA/gold did not bind with the MB and MAG in the eustachian tube, and exclusively bound with MB in the transitional area. Direct binding was also found with MAG and the apical plasmic membrane in the squamous epithelium. These findings indicate that MAG is occluded by MB lined with the interciliary fluid zone for luminal access of lectin at the proximal lumina of the tubotympanic epithelium. It is also suggested that MB existing at two sites possesses a different WGA-binding capacity: shielding as a “dust cover” in the eustachian tube and entrapping as a “flypaper” against lectin in the transitional area of the middle ear.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    European archives of oto-rhino-laryngology and head & neck 250 (1993), S. 412-417 
    ISSN: 1434-4726
    Keywords: Sugar-binding site ; Guinea pig ; Middle ear ; Lipopolysaccharide ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Glucosamine-binding sites were detected in Lowicryl K4M-embedded guinea pig middle ear mucosa by electron microscopy, using glucosaminyl bovine serum albumin. Incubation of ultrathin tissue sections with gold-labeled glucosaminyl bovine serum albumin (GlcN/BSA/gold) resulted in binding mainly on cilia, microvilli, rough endoplasmic reticulum and nuclei. The sugar binding was not inhibited after ultrathin sections had been digested with trypsin or neuraminidase. Various carbohydrates and glycoconjugates were tested as competitive inhibitors of G1cN/BSA/gold labeling on the tissue sections. The sugar specificity range detected by the glucosamine-binding sites included glucosamine, N-acetylglucosamine, mannose and fucose, whereas N-acetylgalactosamine, galactose and glucose were not detectable. A series of endotoxic substances such as Salmonella minnesota Re595 lipid A complex with BSA and lipopolysaccharides (LPS) derived from Escherichia coli 055: B5 or S. minnesota Re595 also competed with GlcN/BSA/gold binding. This indicates that the lipid A backbone glucosamine or other carbohydrate portions of LPS is a part of the structure recognized by glucosamine binding sites.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1435-1536
    Keywords: Neutron Scattering ; Small-angle Scattering ; Al-Zn Alloys ; Guinier-Preston zone ; Age-hardening
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Description / Table of Contents: Zusammenfassung Die Kleinwinkelstreuung der Neutronen von A1-6.8 at % Zn und Al-6.8 at % Zn- 0.1 at % Mg-Legierungen während der Guinier-Preston-Zonen-Bildung wurde am KUR (Kyoto Universität Reaktor) gemessen. Die Proben wurden aus 300 °C in Salzwasser von — 20 °C abgeschreckt und bei 77 K gehalten. Sie wurden bei 40 °C während der verschiedenen Zeitdauer von 0 bis 1000 Minuten mit der nachfolgenden Neutronenmessung bei 77 K gealtert. Die gemessene zeitliche Entwicklung des Streuquerschnittes kann qualitativ erklärt werden in übereinstimmung mit Saitos Formel, die aus zwei verschiedenen Teilen besteht, die der inkohärenten anomalen Schwankung und der kohärenten geordneten Struktur der Konzentration entsprechen.
    Notes: Summary Small-angle neutron scattering from A1-6.8 at % Zn and Al-6.8 at % Zn-0.1 at % Mg alloys during Guinier-Preston zone formation have been measured at KUR (Kyoto University Reactor). The samples were quenched from 300 °C into brine at -20 °C and kept at 77 K. They were aged at 40 °C for various periods ranging from 0 up to 1000 minutes each with subsequent neutron measurement at 77 K. The observed temporal evolution of the cross section can be interpreted qualitatively in terms of Saito's formula which consists of two distinct parts due to the incoherent anomalous fluctuation and to the coherent ordered structure of the concentration.
    Type of Medium: Electronic Resource
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