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  • Acute myelogenous leukemia  (2)
  • Etoposide  (2)
  • Carbohydrate response unit  (1)
  • Chinese Hamster Ovary (CHO) cell lines of different repair capabilities  (1)
  • 1
    Digitale Medien
    Digitale Medien
    An enhancer unit of L-type pyruvate kinase gene is responsible for transcriptional stimulation by dietary fructose as well as glucose in transgenic mice
    Amsterdam : Elsevier
    FEBS Letters 318 (1993), S. 269-272 
    Details
    ISSN: 0014-5793
    Schlagwort(e): Carbohydrate response unit ; L-Type pyruvate kinase gene ; Transgenic mouse
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie , Chemie und Pharmazie , Physik
    Materialart: Digitale Medien
    URL: http://linkinghub.elsevier.com/retrieve/pii/0014-5793(93)80526-Z
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Analysis of the p53 gene mutations in acute myelogenous leukemia: The p53 gene mutations associated with a deletion of chromosome 17 (1995)
    Springer
    Annals of hematology 71 (1995), S. 83-87 
    Details
    ISSN: 1432-0584
    Schlagwort(e): p53 gene ; Acute myelogenous leukemia ; Chromosome 17 ; Polymerase chain reaction-singlestrand conformation polymorphism ; Direct sequencing
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract In order to determine the relevance of the p53 tumor suppressor gene mutations in acute myelogenous leukemia (AML), we analyzed the p53 gene in genomic DNA of 18 unselected cases of AML by polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) and direct sequencing. We detected three cases (16.7%) with the p53 gene mutations showing only the mutant alleles; the high incidence in cases with loss of a whole chromosome 17 (two of three) contrasted with the low incidence in cases without abnormalities of chromosome 17 (one of 15). These cases containing the mutations of the p53 gene showed a poor prognosis. Although we analyzed a rather small series of patients, these findings suggest that the p53 gene mutations might be involved in the progression and prognosis of at least some cases of AML.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01699251
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  • 3
    Digitale Medien
    Digitale Medien
    Reversal effect of itraconazole on adriamycin and etoposide resistance in human leukemia cells (1996)
    Springer
    Annals of hematology 72 (1996), S. 17-21 
    Details
    ISSN: 1432-0584
    Schlagwort(e): Key words Multidrug resistance ; P-glycoprotein ; Itraconazole ; Adriamycin ; Etoposide
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract  Itraconazole is a triazole antifungal agent that inhibits cell membrane serol biosynthesis. Currently, itraconazole is a potent candidate for in vivouse to revert multidrug resistance in acute leukemias, with the added benefit of its antifungal effect. As previously reported, itraconazole, as well as verapamil, reversed adriamycin-resistant K562 cells (K562/ADR) and HL60 cells (HL60/ADR) in dosages compatible to the plasma levels achieved by the therapeutic dosages used for the treatment of fungal infections. By RT-PCR analysis of mdr1, mdr3, and mrp mRNA, these adriamycin-resistant cells showed a higher expression of the transcript of these genes than those of the parent cells. By FACS analysis, both the adriamycin-resistant cells showed a higher expression of P-glycoprotein on their cell surfaces. These results suggested the involvement of itraconazole in the mdr gene and/or mrp gene product-associated resistance. Furthermore, itraconazole partially reversed etoposide resistance in both the K562 and K562/ADR cells. The present study suggests that itraconazole may reverse multidrug resistance, at least in part, via a classical MDR-associated mechanism.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00663011
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  • 4
    Digitale Medien
    Digitale Medien
    Reversal effect of itraconazole on adriamycin and etoposide resistance in human leukemia cells (1996)
    Springer
    Annals of hematology 72 (1996), S. 17-21 
    Details
    ISSN: 1432-0584
    Schlagwort(e): Multidrug resistance ; P-glycoprotein Itraconazole ; Adriamycin ; Etoposide
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Itraconazole is a triazole antifungal agent that inhibits cell membrane serol biosynthesis. Currently, itraconazole is a potent candidate for in vivo use to revert multidrug resistance in acute leukemias, with the added benefit of its antifungal effect. As previously reported, itraconazole, as well as verapamil, reversed adriamycin-resistant K562 cells (K562/ADR) and HL60 cells (HL60/ADR) in dosages compatible to the plasma levels achieved by the therapeutic dosages used for the treatment of fungal infections. By RT-PCR analysis of mdrl, mdr3, and mrp mRNA, these adriamycin-resistant cells showed a higher expression of the transcript of these genes than those of the parent cells. By FACS analysis, both the adriamycin-resistant cells showed a higher expression of P-glycoprotein on their cell surfaces. These results suggested the involvement of itraconazole in the mdr gene and/or mrp gene product-associated resistance. Furthermore, itraconazole partially reversed etoposide resistance in both the K562 and K562/ADR cells. The present study suggests that itraconazole may reverse multidrug resistance, at least in part, via a classical MDR-associated mechanism.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00663011
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    Analysis of the p53 gene mutations in acute myelogenous leukemia: the p53 gene mutations associated with a deletion of chromosome 17 (1995)
    Springer
    Annals of hematology 71 (1995), S. 83-87 
    Details
    ISSN: 1432-0584
    Schlagwort(e): Key words p53 gene ; Acute myelogenous leukemia ; Chromosome 17 ; Polymerase chain reaction ; single-strand conformation polymorphism ; Direct sequencing
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract  In order to determine the relevance of the p53 tumor suppressor gene mutations in acute myelogenous leukemia (AML), we analyzed the p53 gene in genomic DNA of 18 unselected cases of AML by polymerase chain reaction–single-strand conformation polymorphism (PCR–SSCP) and direct sequencing. We detected three cases (16.7%) with the p53 gene mutations showing only the mutant alleles; the high incidence in cases with loss of a whole chromosome 17 (two of three) contrasted with the low incidence in cases without abnormalities of chromosome 17 (one of 15). These cases containing the mutations of the p53 gene showed a poor prognosis. Although we analyzed a rather small series of patients, these findings suggest that the p53 gene mutations might be involved in the progression and prognosis of at least some cases of AML.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01699251
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 6
    Digitale Medien
    Digitale Medien
    Green Fluorescent Protein (GFP) as a Marker for Cell Viability After UV Irradiation (1999)
    Springer
    Journal of fluorescence 9 (1999), S. 37-43 
    Details
    ISSN: 1573-4994
    Schlagwort(e): Green fluorescent protein (GFP) ; constitutive GFP expression ; visualization of GFP fluorescence ; UV exposure of mammalian cells ; Chinese Hamster Ovary (CHO) cell lines of different repair capabilities
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract Generation of Chinese Hamster Ovary (CHO) cell lines stably expressing green fluorescent protein (GFP) was achieved using a plasmid vector that encoded the red-shifted pCX-xGFP under the control of a strong hybrid promoter composed of a CMV enhancer and a β-actin/β-globin gene promoter. Cotransfection of the promoter-less pSV2-Neo helper plasmid transmitting neomycin resistance was followed by selection with the antibiotic G418. Constitutive GFP expression could be visualized in living and fixed cells using fluorescence spectroscopy, fluorescence microscopy, and flow cytometry. DNA repair-proficient (AA8) and deficient (UV5) CHO strains were used for survival tests after UVC irradiation. Cells carrying the GFP construct (AA8-pGFP, UV5-pGFP) show the same response to UV irradiation (colony forming ability) as their nontransformed parental cell lines (AA8, UV5). Using GFP as a marker for cell viability, cells were harvested after certain postirradiation growth periods and the numbers of GFP expressing cells and fluorescence intensities were determined by FACS analysis. Generally, GFP fluorescence in irradiated cells is not seen when cell membranes are damaged (leak-out of the soluble GFP). Irradiated cells without membrane damage express GFP continuously (leading to a dose-dependent increase in GFP contents).
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1020583623407
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