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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Research in experimental medicine 164 (1974), S. 89-96 
    ISSN: 1433-8580
    Keywords: Interference microscopy ; Vitalmicroscopic technique ; Connective tissue, structure ; Perivascular space ; Interferenzmikroskopie ; Vitalmikroskopische Technik ; Bindegewebsstruktur ; Perivasalraum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Mit interferenzmikroskopischer Technik wurde die ausgespannte Mesenterialplatte einer vorgelagerten Dünndarmschlinge der weißen Ratte im Vitalmikroskop untersucht. Die optischen Bedingungen der beobachteten Interferenzphänomene werden diskutiert. Im interstitiellen Gewebe fanden sich zwei Strukturelemente, die sich hinsichtlich ihrer Gangunterschiede differenzieren ließen. Sie werden den Faserstrukturen und der Grundsubstanz des Bindegewebes zugeordnet. Systematische Abweichungen der Interferenzerscheinungen in Beziehung zum Abstand von den capillären Blutgefäßen fanden sich nicht, so daß auf eine gleichmäßige Dicke des perivasalen Gewebes im Objekt geschlossen wird.
    Notes: Summary Using the interference technique the mesenterial plate of the rat was studied vitalmicroscopically. The optical conditions leading to the observed interference colours are discussed. By their path differences two tissue elements could be distinguished. They correspond to fibrous structures and the ground substance of the connective tissue. The interference phenomena did not show any systematic relationship to the capillary blood vessels. Therefore, the thickness of the perivascular tissue in the observed object seems to be uniform.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Research in experimental medicine 184 (1984), S. 1-15 
    ISSN: 1433-8580
    Keywords: Capillary permeability ; Intravital microscopy ; Fluorescence ; Perivascular diffusion ; TV microscope
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The transport of fluorescent tracer molecules of various molecular weights (MW 340-300,000) within the blood vessels and through the vessel walls into the perivascular tissue of the rat mesentery was studied microscopically. Using a highly sensitive TV-tube the fluorescence intensities were transformed into video signals and recorded for subsequent analysis. The results show that small molecules, such as the water-soluble dye fluorescein-sodium (FlNa) (MW 340) can pass through the wall of all blood vessels with minimal delay, whereas the passage of large molecules, such as the serum proteins (MW 〉 60,000), depends very strongly on the diameter of the vessels and on the medium in which the mesentery is embedded during the observation. In this respect, no difference was registered between vessels of the arterial and of the venous part of the microcirculation. The large serum protein molecules moved through the blood vessel walls at specific leakage points. The small dye molecules, however, traversed the wall uniformly along the entire length of the blood vessel. The small-molecule behavior could be described by a passive diffusion model with a cylindrical diffusion source.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Clinical and experimental medicine 154 (1971), S. 334-338 
    ISSN: 1591-9528
    Keywords: Ultraviolet microspectrophotometry ; Quantitative capillary microscopy ; Vitalmicroscopic technique ; Microscopic object chamber ; Ultraviolett-Mikrospektrophotometrie ; Quantitative Capillarmikroskopie ; Vitalmikroskopische Technik ; Mikroskopische Objektkammer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die Capillarmikroskopie im ultravioletten Strahlenbereich ist durch die Einführung bildwandelnder Systeme praktisch vereinfacht worden. Damit ist auch der Weg für spektrophotometrische Untersuchungen bei kurzen Wellenlängen eröffnet. Für diesen Zweck wird eine Objektkammer angegeben, mit der transparente Objekte bei optimaler Beleuchtung untersucht werden können. Die Einzelheiten der Kammer und die Technik der Objektpräparation (Rattenmesenterium) werden beschrieben.
    Notes: Summary The capillary microscopy in the ultraviolet range has been improved by the development of image-converting systems. It became possible to make spectrophotometric measurements in the short wavelength range. For this purpose we constructed an object chamber, suited for transparent tissues under optimal microscopic illumination. The details of the chamber and the technique of the tissue preparation (rat's mesenterium) are described.
    Type of Medium: Electronic Resource
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