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  • Dynein heavy chain related polypeptides  (1)
  • Gamete interaction  (1)
  • 1
    Electronic Resource
    Electronic Resource
    In vitro double fertilization in Nicotiana tabacum (L.): fusion behavior and gamete interaction traced by video-enhanced microscopy (2000)
    Springer
    Sexual plant reproduction 12 (2000), S. 267-275 
    Details
    ISSN: 1432-2145
    Keywords: Key words Nicotiana tabacum ; In vitro fertilization ; Gamete interaction ; Cytology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  In vitro double fertilization in tobacco was carried out with attention to fusion behavior and gamete interaction. Structural and cytological events indicating possible reaction to the fusion of sperm-egg and especially sperm-central cell were recorded by video-enhanced microscopy. Generative cells were fused with the egg cell or central cell as a control system to better understand gamete interaction. As early as adherence of the male cell, the female cell showed response by means of cytoplasm strand formation. After gamete fusion, cytoplasm activation in the egg cell was observed as long distance movement of organelles. In fertilized central cells, however, fusion did not result in notable cytological change within 30 min. Male nuclear movement recorded in the female cell illustrated two different patterns of movement which showed similarity to organelle movement. The dynamics of male and female nuclear fusion after in vitro fertilization was also recorded in the central cell. It revealed that the fusion process requires only a few seconds and is similar to that of gamete fusion in vitro. This may offer a new clue for understanding how female and male nuclei attract, adhere and finally fuse each other.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004970050194
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Dynein heavy chain-related polypeptides are associated with organelles in pollen tubes of Nicotiana tabacum (1998)
    Springer
    Sexual plant reproduction 11 (1998), S. 31-40 
    Details
    ISSN: 1432-2145
    Keywords: Key words Pollen tube ; Nicotiana tabacum ; Dynein heavy chain related polypeptides ; Immunolocalization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  It is known that pollen tubes contain two high molecular weight polypeptides which share some biochemical and immunological properties with dynein heavy chains. This paper reports data on the subcellular localization of the two dynein heavy chain-related polypeptides during pollen tube growth. Immunofluoresence studies using a purified antibody (Dy-1) raised against a synthetic peptide reproducting the P-loop conserved sequence of dynein heavy chains showed spot-like structures, with a characteristic distribution pattern that depended on the tube length. Biochemical evidence confirmed the presence of dynein heavy chain-related bands in the pollen tube membrane fraction. The association of proteins carrying dynein heavy chain-related polypeptides to cell membranes was affected by detergent (Triton×100), whereas other stripping agents, like NaCl and Na2CO3, did not significantly influence the interaction of dynein heavy chain-related doublet with their cytoplasmic targets. These data suggest that dynein heavy chain-related polypeptides associate with membranous organelles within the vegetative cell of Nicotiana tabacum pollen tubes, implying their involvement in the cytoplasmic distribution of these organelles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004970050118
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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