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  • 1
    Electronic Resource
    Electronic Resource
    Genetic relationships in Lens species and parentage determination of their interspecific hybrids using RAPD markers (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 1091-1098 
    Details
    ISSN: 1432-2242
    Keywords: Lens culinaris ; Wild species ; RAPD ; Intra- and interspecific variation ; Genetic distance ; Split decomposition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Broadening of the genetic base and systematic exploitation of heterosis in cultivated lentils requires reliable information on genetic diversity in the germplasm. The ability of random amplified polymorphic DNA (RAPD) to distinguish among different taxa of Lens was evaluated for several geographically dispersed accessions/cultivars of four diploid Lens species. This study was carried out to assess whether RAPD data can provide additional evidence about the origin of the cultivated lentil and to measure genetic variability in lentil germplasm. Three cultivars of Lens culinaris ssp. culinaris, including one microsperma, and two macrosperma types, and four wild species (L. culinaris ssp. orientalis, L. odemensis and L. nigricans) were evaluated for genetic variability using a set of 1 11-mer and 14 random 10-mer primers. One hundred and fifty-eight reproducible and scorable DNA bands were observed from these primers. Genetic distances between each of the accessions were calculated from simple matching coefficients. Split decomposition analysis of the RAPD data allowed construction of an unrooted tree. This study revealed that (1) the level of intraspecific genetic variation in cultivated lentils is narrower than that in some wild species. (2) L. culinaris ssp. orientalis is the most likely candidate as a progenitor of the cultivated species, (3) L. nigricans accession W6 3222 (unknown) and L. c. ssp. orientalis W6 3244 (Turkey) can be reclassified as species of L. odemensis and (4) transmission of genetic material in Lens interspecific hybrids is genotypically specific, as identified by the RAPD markers in our study.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224054
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    IBPGR morphological descriptors — their relevance in determining patterns within a diverse spring barley germplasm collection (1992)
    Springer
    Theoretical and applied genetics 85 (1992), S. 489-495 
    Details
    ISSN: 1432-2242
    Keywords: IBPGR ; Morphological descriptors ; Genetic diversity ; Barley
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The International Board for Plant Genetic Resources (IBPGR) promotes a minimum set of morphological characters thought satisfactory for the custodial management of crop germplasm collections. The purpose of such conserved germplasm is as a genetic resource for future plant breeding programmes. Because future plant breeding requirements are not always known, the curator's strategy in maintaining an adequate germplasm resource is to conserve as wide a range of genetic diversity as possible. How is diversity measured to ensure a wide range of conserved germplasm? The IBPGR minimum descriptors detail genetic diversity at particular points in a genome corresponding to the observed characters. The purpose of the present study was to investigate whether diversity as identified by the IBPGR minimum set of descriptors could yield satisfactory measures of diversity in a contrasting set of genomic markers. A diverse spring-sown barley collection of 1379 cultivars was evaluated for the 12 IBPGR minimum taxonomic characters. An additional nine phenotypic characters and six biochemical markers were evaluated to enable diversity comparisons. Cluster analysis of the various sets of data revealed groups of accessions for each of the three data sets. A poor level of agreement (congruence) between data sets was observed in all comparisons indicating that, for cultivated barley at least, diverse collections according to the IBPGR minimum descriptors is not necessarily related to equivalent levels of diversity in other genetic characters. Implications of the relevance of the IBPGR descriptor list and appropriate collection strategies are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222331
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Genetic relationships in Lens species and parentage determination of their interspecific hybrids using RAPD markers (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 1091-1098 
    Details
    ISSN: 1432-2242
    Keywords: Key words Lens culinaris ; Wild species ; RAPD ; Intra- and interspecific variation ; Genetic distance ; Split decomposition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Broadening of the genetic base and systematic exploitation of heterosis in cultivated lentils requires reliable information on genetic diversity in the germ-plasm. The ability of random amplified polymorphic DNA (RAPD) to distinguish among different taxa of Lens was evaluated for several geographically dispersed accessions/cultivars of four diploid Lens species. This study was carried out to assess whether RAPD data can provide additional evidence about the origin of the cultivated lentil and to measure genetic variability in lentil germplasm. Three cultivars of Lens culinaris ssp. culinaris, including one microsperma, and two macrosperma types, and four wild species (L. culinaris ssp. orientalis, L. odemensis and L. nigricans) were evaluated for genetic variability using a set of 1 11-mer and 14 random 10-mer primers. One hundred and fifty-eight reproducible and scorable DNA bands were observed from these primers. Genetic distances between each of the accessions were calculated from simple matching coefficients. Split decomposition analysis of the RAPD data allowed construction of an unrooted tree. This study revealed that (1) the level of intraspecific genetic variation in cultivated lentils is narrower than that in some wild species. (2) L. culinaris ssp. orientalis is the most likely candidate as a progenitor of the cultivated species, (3) L. nigricans accession W6 3222 (unknown) and L. c. ssp. orientalis W6 3244 (Turkey) can be reclassified as species of L. odemensis and (4) transmission of genetic material in Lens interspecific hybrids is genotypically specific, as identified by the RAPD markers in our study.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050235
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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