Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Electronic Resource  (9)
  • Immunohistochemistry  (6)
  • Interstitial nucleus of Cajal  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Islet amyloid polypeptide in insulinoma and in the islets of the pancreas of non-diabetic and diabetic subjects (1991)
    Springer
    Virchows Archiv 418 (1991), S. 411-417 
    Details
    ISSN: 1432-2307
    Keywords: Islet amyloid polypeptide ; Insulinoma ; Pancreatic islet ; Diabetes mellitus ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Amyloid deposition is a common pathological feature in insulinoma and in the islets of the pancreas in type-2 diabetic patients. The present immunohisto-chemical study revealed that normal B-cells, insulinoma, and amyloid deposits in insulinoma and diabetic pancreatic islets were commonly immunoreactive with antiserum to C-terminal synthetic tetradecapeptide of human islet amyloid polypeptide (IAPP) (24–37). Amyloid fibrils in insulinoma were also positive to IAPP by immunoelectron microscopy. A high level of IAPP was detected in the plasma and tissue of a insulinoma patient by radioimmunoassay suggesting that amyloid deposition in insulinoma is due to overproduction of IAPP. Amyloid deposits immunoreactive to IAPP were also seen in all diabetic pancreatic islets, but in no non-diabetic islets. There was much amyloid deposition in the islets of severe diabetics, whose B-cells demonstrated decreased immunoreactivities for IAPP and insulin. The IAPP content of the pancreas was 649.0 and 847.7 pg/mg wet weight in each of two diabetic patients, and 1034.6 and 1447.7 pg/mg wet weight in two non-diabetic patients. The present study revealed that IAPP is a bioactive peptide secreted from islet B-cells and are amyloidogenic peptide concerned in diabetogenensis and/or the progression of type-2 diabetes mellitus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01605927
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Alzheimer's neurofibrillary tangles are penetrated by astroglial processes and appear eosinophilic in their final stages (1987)
    Springer
    Acta neuropathologica 72 (1987), S. 214-217 
    Details
    ISSN: 1432-0533
    Keywords: Neurofibrillary tangles ; Senile dementia of Alzheimer type ; Glial fibrillary acidic protein ; Astrocytes ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Alzheimer's neurofibrillary tangles (ANT) in the hippocampal area were studied immunohistochemically using antisera against glial fibrillary acidic protein (GFAP) and S-100 protein in 48 patients with or without dementia between 52 and 92 years old. In 27 of the 38 brains that developed ANT in the hippocampal area, some ANT were immunostained with these antisera. Flame-shaped or globose-shaped immunostains were occasionally continuous with astroglial cell bodies and processes. They appeared particularly in the entorhinal cortex, subiculum and CAl. The ANT, immunostained with GFAP and S-100 antisera, apparently correspond to slightly eosinophilic tangles in H&E sections and to less argentophilic tangles in silver-impregnated sections in all of the 27 brains. ANT of another 11 brains were consistently negative with these antisera. The GFAP-positive eosinophilic tangles were encountered in the brains of older patients (P〈0.01) and with more abundant formation of ANT (P〈0.001). This alteration was present in all of the 20 brains with more than 100 ANT per section and none of the eight brains with less than 10 ANT. These findings suggest that in the last stages, ANT are penetrated by eosinophilic processes of astrocytes, and appear eosinophilic, and that the presence of GFAP-positive eosinophilic tangles indicates the abundant formation of ANT.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00691092
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    An immunohistochemical study on HLA-DR expression in human meningiomas (1992)
    Springer
    Acta neuropathologica 84 (1992), S. 110-112 
    Details
    ISSN: 1432-0533
    Keywords: HLA-DR ; Meningioma ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The expression of HLA-DR was examined in 38 cases of meningiomas with the streptavidin-biotin immunoperoxidase method using two monoclonal antibodies to HLA-DR (LN-3 and TAL-IB5) on formalinfixed, paraffin-embedded specimens. Similar immunoreactivity was obtained with these two monoclonal antibodies. In addition to infiltrated lymphoid cells and perivascular macrophages, tumor cells themselves showed HLA-DR expression in 16 cases (42%) of meningiomas. The rate of HLA-DR-positive cases in the transitional and fibrous subtypes (64% and 67%, respectively) was higher than that in the meningotheliomatous subtype (8%). Spindle-shaped tumor cells were frequently positive for HLA-DR, whereas few of meningotheliomatous cells with plump cytoplasm were positive. Most of HLA-DR-positive cases showed no or scanty lymphoid cell infiltration, and a few cases with marked infiltration of lymphoid cells were variable for HLA-DR expression. These findings suggest little correlation between HLA-DR expression of tumor cells and the degree of lymphoid cell infiltration, but indicate an aberrant HLA-DR expression of tumor cells themselves.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00427224
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Immunohistochemical study on the distribution of α and β subunits of S-100 protein in brain tumors (1991)
    Springer
    Acta neuropathologica 81 (1991), S. 657-663 
    Details
    ISSN: 1432-0533
    Keywords: Brain tumor ; S-100 protein ; Subunit ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The immunohistochemical distribution of α and β subunits of S-100 protein (S-100α, S-100β, respectively) in 138 cases of human brain tumors was investigated by the avidin-biotin immunoperoxidase method. Brain tumors can be divided into four groups: group 1 [S-100α (+) and/or S-100β (+)]; astrocytoma, glioblastoma, ependymoma, subependymoma, oligodendroglioma, choroid plexus papilloma, gangliocytoma, meningioma, chordoma, malignant melanoma. Group 2 [S-100α (+) and S-100β (-)]; pineoblastoma, pituitary adenoma, craniopharyngioma, rhabdomyosarcoma. Group 3 [S-100α (-) and S-100β (+)]; acoustic Schwannoma. Group 4 [S-100α (-) and S-100β (-)]; medulloblastoma, malignant lymphoma, germinoma. The S-100β immunoreactivity pattern in brain tumors was similar to those obtained using conventional anti-S-100 protein sera. In the first group of brain tumors both the number of positively stained tumor cells and the staining intensity were generally greater for S-100β than for S-100α with a few exceptions including one gemistocytic astrocytoma, one subependymoma, one malignant melanoma, and some cases of glioblastomas. As to the relationship between malignancy and S-100 protein in glioma, S-100β immunoreactivity decreased according to degree of malignancy, while that of S-100α varied, suggesting a heterogeneity of tumor cells in glioblastomas. Immunostaining for S-100α and S-100β might become a useful diagnostic procedure in brain tumors and may give us more detailed and precise data of S-100 protein in brain tumors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00296376
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Interstitial-vestibular interaction in the control of head posture (1985)
    Springer
    Experimental brain research 57 (1985), S. 264-270 
    Details
    ISSN: 1432-1106
    Keywords: Interstitial nucleus of Cajal ; Head posture ; Interstitial vestibular interaction ; Vestibular compensation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. Experiments were performed in cats to determine whether the head tilt following a unilateral lesion of the interstitial nucleus of Cajal (INC) can be attributed to removal of interstitiospihal fibers which have direct excitatory synaptic connections with ipsilateral neck extensor (biventer cervicis-complexus) and flexor (sternocleidomastoid, SCM) motoneurons. Unilateral INC lesions were made either electrolytically or reversibly by procaine infusion into the INC, and electromyographic activity was recorded bilaterally from biventer (BIV), splenius (SP) and SCM muscles. In both groups of lesions, activity of the ipsilateral SP and BIV was higher than that of the contralateral ones. When procaine was infused into the INC of awake cats, an increase of activity of the ipsilateral SP began before the cats presented the typical head tilt to the opposite side. Bilateral INC lesions caused dorsiflexion of the head. These results indicate that the head tilt resulting from unilateral INC lesions can not be explained by simple removal of the ipsilateral, direct excitatory interstitioneck impulses. 2. When unilateral INC lesions were combined with hemilabyrinthectomies, cats that were given labyrinthectomies on the side opposite to the previous INC lesions showed very severe head tilt, whereas cats that received labyrinthectomies on the same side did not show obvious head tilt. Furthermore, it took a much longer time for the cats of the former group to compensate the head tilt than it took those that had single lesions of the INC or labyrinth. These results suggest that the INC and labyrinth interact in the control of head posture and that the INC also plays a role in vestibular compensation. However, when bilatral INC lesions were combined with hemilabyrinthectomies, cats that had previously received bilateral INC lesions and which had fully compensated the head posture recuperated from vestibular symptoms following hemilabyrinthectomy within one to two weeks. Moreover, bilateral INC lesions that were performed in cats which had previously been given hemilabyrinthectomies and in which vestibular symptoms were well compensated did not produce any recurrence of vestibular symptoms. These results indicate that although the INC plays a role in the control of head posture following hemilabyrinthectomy, it is not needed for coarse vestibular compensation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236531
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Effects of lesion of the interstitial nucleus of Cajal on vestibular nuclear neurons activated by vertical vestibular stimulation (1986)
    Springer
    Experimental brain research 64 (1986), S. 496-504 
    Details
    ISSN: 1432-1106
    Keywords: Interstitial nucleus of Cajal ; Reversible lesion ; Vestibular nuclear neurons ; Vertical semicircular canal ; Vertical vestibuloocular reflex
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 1. Experiments were performed in cats anesthetized with nitrous oxide to study the effects of INC lesions on responses of vestibular nuclear neurons during sinusoidal rotations of the head in the vertical (pitch) plane. Responses of neurons in the INC region were recorded during pitch rotations at 0.15 Hz. A great majority of these neurons did not respond to static pitch tilts, and they seemed to respond either to anterior or to posterior semicircular canal inputs with a peak phase lag of 140 deg (re head acceleration). 2. Responses of vestibular nuclei neurons in intact cats were recorded during pitch rotations at the same frequency (0.15 Hz). Neurons that seemed to respond to vertical semicircular canal inputs showed peak phase lags of 90 deg relative to head acceleration, whereas neurons that responded to static pitch tilts showed peak phase shifts near 0 deg. These results indicate that responses of neurons in the INC region lag those of vestibular neurons by about 50 deg, suggesting that the former neurons possess a phase-lagging (i.e. integrated) vestibular signal. 3. Responses of vestibular neurons in cats that had received electrolytic lesions of bilateral INCs 1–2 weeks previously were recorded during pitch rotations at the same frequency (0.15 Hz). Neurons that presumably responded to vertical semicircular canal inputs showed a peak phase lag of 60 deg relative to head acceleration, a significant decrease of the phase lag compared to normal, whereas responses near 0 deg were unchanged. Gain values of individual cells also significantly dropped from 2.07 ± 0.67 spikes · s−1/deg · s−22 (mean ± SD; normal cats) to 1.27 ± 0.68 spikes · s−2/deg · s−2 (INC lesioned cats) at 0.15 Hz. When responses of vestibular neurons were studied during pitch rotations in the range of 0.044–0.49 Hz in these cats, a large decrease of the phase lag was observed at lower frequencies, whereas the slopes of phase lag curves of vestibular neurons in intact cats were rather flat. 4. Procaine infusion into the bilateral INCs not only resulted in a decrease of 20–50 deg in the phase lag in responses of vestibular neurons that had lagged head acceleration by 90–140 deg before procaine infusion, but also dropped the gain of the response to rotation by an average of 31%, whereas responses of neurons that had showed phase shifts near 0 deg were not influenced consistently. Simultaneous recording of the vestibular neurons and the vertical vestibuloocular reflex (VOR) indicated that the phase advance and gain drop of vestibular neurons occurred earlier than those of the VOR. These results exclude the possibility that the change in dynamic response of vestibular neurons after procaine infusion is due to depression of general brain stem activity that may lead to the phase advance of the VOR, and suggest that the decrease of the phase lag and gain drop in responses of the vestibular neurons was caused by removal of the phase-lagging, feedback signal coming from the INC to the vestibular nuclei.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00340487
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Responses of vestibular neurons to stimulation of the interstitial nucleus of cajal in the cat (1983)
    Springer
    Experimental brain research 51 (1983), S. 1-15 
    Details
    ISSN: 1432-1106
    Keywords: Midbrain reticular formation ; Interstitial nucleus of Cajal ; Vestibular neurons ; Multiple branching ; Vestibulo-collic reflexes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary (1) Spikes of neurons in the medial and descending vestibular nuclei were recorded extracellularly and their responses to stimulation of the interstitial nucleus of Cajal (INC) were studied in cerebellectomized cats under chloralose anesthesia. Stimuli applied in the ipsilateral INC excited 37% of neurons that did not exhibit spontaneous activity. About 84% of spontaneously discharging neurons were influenced by the INC; typical responses were excitation (35%), inhibition (22%) and excitation followed by inhibition (27%). Of the neurons that were excited, 24% fired monosynaptically. Such monosynaptic activation was evoked by stimulating the INC and midbrain medial longitudinal fasciculus (MLF), but was not evoked by stimulating the lateral midbrain reticular formation. Polysynaptic excitation or inhibition was evoked more widely, but the lowest threshold points were within the INC. Stimulation of the contralateral INC also evoked polysynaptic excitation or inhibition. However, the frequency of occurrence of the evoked responses was significantly smaller compared to the ipsilateral responses. (2) Intracellular recordings revealed that some medial and lateral vestibular neurons received monosynaptic excitatory postsynaptic potentials (EPSPs), others received polysynaptic EPSPs or inhibitory postsynaptic potentials (IPSPs) from the ipsilateral INC. The minimum latency for the IPSPs suggests that the pathway is at least disynaptic. No significant collision was observed between monosynaptic EPSPs evoked by the ipsilateral INC and contralateral vestibular nuclei. Acute lesions that damaged the pontine MLF and part of the reticular formation did not abolish monosynaptic responses of vestibular neurons by the INC. Depth threshold curves for mono- or polysynaptic responses drawn before and after the lesions were virtually similar. Antidromic thresholds of interstitio-vestibular fibers evoked from the pontine MLF showed that a great majority of these fibers run outside the MLF at the pontine level. These results control for vestibular axon reflexes, since vestibulo-interstitial fibers ascend within the MLF (cf. Gacek 1971). (3) Responses to stimulation of the INC were not different among different types of canal responding neurons; vertical and horizontal canal responding neurons received similar effects. However, canal responding neurons that received excitation from the contralateral vestibular nerve were activated more frequently by the INC than those that received inhibition (χ2 test, p〈0.01). Qualitatively similar results were obtained from vestibular neurons that had different projection sites; vestibulospinal, contralateral INC-projecting and contralateral vestibular nuclei-projecting neurons received similar effects. (4) Vestibulo-collic reflexes, studied with EMG, were modified by preceding INC stimulation. Intracellular recordings from some neck motoneurons showed that disynaptic EPSPs evoked by stimulation of the contralateral vestibular nerve were modified by preceding INC stimulation applied ipsilateral to the stimulated vestibular nerve. INC stimulation alone did not evoke any response in these motoneurons, suggesting that the interaction of the labyrinthine and interstitial effects occurred at least in part at the vestibular nuclei. (5) Some medial and descending vestibular neurons showed multiple branching, projecting to the contralateral INC, C1 or contralateral vestibular nuclei. About 34% of neurons that projected to the contralateral INC were also antidromically activated from the C1; some of them received vertical canal inputs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236797
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    The occurrence of IgG in the endolymphatic sac of the guinea pig (1987)
    Springer
    European archives of oto-rhino-laryngology and head & neck 243 (1987), S. 370-373 
    Details
    ISSN: 1434-4726
    Keywords: Immunoglobulin G ; Inner ear ; Endolymphatic sac ; Immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We investigated the occurrence of immunoglobulin G (IgG) in the endolymphatic sac (ES) of the guinea pig in order to show that the ES is an organ involved in the immunoreactivity of the inner ear. By using an immunohistological technique, we were able to show that IgG is located mainly in the subepithelial layer of the ES. We also found that IgG is present in some epithelial cells of the ES as well as in free floating cells in the ES lumen. Although plasma cells have been described previously in the subepithelial layer, none could be recognized in this layer of the ES in our specimens. Our results suggest that the ES is an immunoreactive organ of the inner ear in some pathological states, while IgG in the normal ES is primarily of systemic origin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00464644
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    Distribution of murine mannose receptor expression from early embryogenesis through to adulthood (1998)
    Springer
    Cell & tissue research 292 (1998), S. 311-323 
    Details
    ISSN: 1432-0878
    Keywords: Key words Mannose receptor ; Macrophage-specific antigen F4/80 ; Macrophages ; Endothelial cells Embryogenesis ; Development ; Immunohistochemistry ; Mouse (C57Black/6 ; BALB/c)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  The mannose receptor is a 175-kDa transmembrane glycoprotein that appears to be expressed on the surface of terminally differentiated macrophages and Langerhans cells. The ectodomain of the mannose receptor has eight carbohydrate recognition domains. The receptor recognizes the patterns of sugars that adorn a wide array of bacteria, parasites, yeast, fungi, and mannosylated ligands. Clearance studies in whole animals have localized radiolabeled ligands, such as mannosylated bovine serum albumen, not only to macrophages, but also to liver sinusoidal endothelial cells. Hitherto, there has been no comprehensive analysis of expression of the mannose receptor in embryonic and adult mouse tissues. In this study, we have undertaken a systematic survey of the expression of the mannose receptor from early embryogenesis through to adulthood. The mannose receptor is expressed on tissue macrophages throughout the adult mouse as expected. However, the mannose receptor is first observed on embryonic day 9 on cells that line blood island vessel walls in the yolk sac. The mannose receptor is localized on sinusoidal endothelial cells in embryonic liver by embryonic day 11 and in bone marrow at embryonic day 17. This pattern persists in these organs throughout embryogenesis into adulthood when sinusoidal endothelial cells of lymph nodes also express the mannose receptor. The receptor is also found on lymphatic endothelial cells of small intestine. In contrast, sinusoids of spleen and thymus do not express mannose receptor antigen. This study demonstrates that the mannose receptor is expressed on tissue macrophages and on subsets of vascular and lymphatic endothelial cells. Thus, the mannose receptor maybe a marker of the so-called reticuloendothelial system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410051062
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...