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  • 1
    Electronic Resource
    Electronic Resource
    Inhibitory effects of 4,4′-diisothiocyano stilbene-2,2′-disulfonic acid (DIDS) in the response of isolated hepatocytes to phalloidin (1978)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 304 (1978), S. 303-307 
    Details
    ISSN: 1432-1912
    Keywords: Phalloidin poisoning ; Isolated hepatocytes ; 4,4′-diisothiocyano stilbene-2,2′-disulfonic acid ; Plasma membranes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 4,4′-Diisothiocyano stilbene-2,2′-disulfonic acid (DIDS) inhibits the typical development of protrusions, regularly seen after treatment of isolated hepatocytes with phalloidin. The degree of inhibition depends on the time of preincubation and on the concentration of DIDS, but not on the concentration of phalloidin. DIDS is more effective than H2DIDS. The inhibition by both compounds is irreversible. The binding capacity of hepatocytes for H2DIDS is much higher than that of the phalloidin-insensitive hepatoma cells. Gel electrophoresis of lysates from cells, pretreated with 3H2DIDS demonstrates that actin binds very little of the inhibitor. Our results suggest that a protein structure on the surface of hepatocytes, needed for the response to phalloidin, is influenced by DIDS or H2DIDS.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00507973
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Uptake of 3H-demethylphalloin by isolated hepatocytes in the presence of various concentrations of phalloin or phalloidin (1980)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 311 (1980), S. 91-94 
    Details
    ISSN: 1432-1912
    Keywords: Phalloidin ; Phalloin ; Demethylphalloin ; Isolated hepatocytes ; Uptake kinetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The uptake of trace amounts of 3H-demethylphalloin (3H-DMP) by isolated hepatocytes was studied in the presence of various concentrations of unlabeled demethylphalloin (DMP), of phalloin and of phalloidin. The addition of phalloidin (or phalloin) reduces the uptake of 3H-DMP more than the addition of the equivalent concentration of DMP. The error caused by dilution of 3H-DMP with phalloin or phalloidin is not constant and depends on the concentration of the unlabeled compound. The relative differences between the uptake of 3H-DMP in the presence of demethylphalloin and in the presence of either phalloin or phalloidin cannot be explained by a competitive model. Some consequences for the use of 3H-DMP in toxicokinetic experiments are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00500308
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Is ligandin relevant for the uptake and storage of phallotoxins in liver cell? (1981)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 317 (1981), S. 364-367 
    Details
    ISSN: 1432-1912
    Keywords: Isolated hepatocytes ; Phalloidin ; Ligandin ; Protein binding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary To exclude an involvement of ligandin in the uptake and storage of phalloidin in hepatocytes equilibriumdialysis studies were made with phalloidin, cholic acid and bromosulfophthalein (BSP). Binding studies with isolated ligandin indicated that the affinity of ligandin for phalloidin is low (K D=0.8×10−3 M). Phalloidin neither displaced BSP (K D=1.3×10−7M) or cholic acid (K D=7.6×10−5 M) from ligandin, when preloaded with these substrates. Hepatocytes prepared from rats after daily treatment with phenobarbital during 5 days contained 3–4-fold concentrations of ligandin and bound greater amounts of BSP than controls. Nevertheless the velocity of the uptake both of [3H]-demethylphalloin ([3H]-DMP) and of [35S]-BSP was not augmented. Also the sensitivity of liver cells to phalloidin was not drastically modified after induction with phenobarbital and agrees with earlier findings in vivo. We conclude that ligandin plays a negligible role in the uptake and a minor role in the storage of phallotoxins in liver cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00501320
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    The role of bile acids in phalloidin poisoning (1977)
    Springer
    Naunyn-Schmiedeberg's archives of pharmacology 301 (1977), S. 145-147 
    Details
    ISSN: 1432-1912
    Keywords: Phalloidin poisoning ; Isolated hepatocytes ; Bile acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Glycocholate and other bile acids inhibit the response of isolated hepatocytes to phalloidin in a concentration dependent manner. It is suggested that the inhibition is due to a block of phalloidin uptake. This interaction might explain the high specificity of phalloidin for liver tissue.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00501430
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    Paper (German National Licenses)
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