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  • 1
    Electronic Resource
    Electronic Resource
    Stable transformation of Eustoma grandiflorum by particle bombardment (1998)
    Springer
    Plant cell reports 17 (1998), S. 504-507 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsEustoma grandiflorum ; Particle bombardment ; Transgenic plant ; bar
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Explants (7.5±2.5 mm) cut from stems and roots of 3-week-old Eustoma grandiflorum Grise, (lisianthus) cv. Glory White seedlings were bombarded with plasmid pBI221, which harbors the uidA gene encoding β-glucuronidase (GUS) driven by the cauliflower mosaic virus (CaMV) 35S promoter. More than 800 blue spots of GUS-expressing cells were observed per 90 explants. Explants bombarded with pARK22 harboring the bar gene encoding phosphinothricin acetyltransferase driven by the CaMV 35S promoter were selected for bialaphos resistance. Putative transgenic plants were obtained about 3 months after bombardment. Southern blot analysis of putative transgenic plants revealed the presence of the bar gene in their genome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050432
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Fluorescent banding pattern analysis of eight taxa of Phaseolus and Vigna in relation to their phylogenetic relationships (1993)
    Springer
    Theoretical and applied genetics 87 (1993), S. 38-43 
    Details
    ISSN: 1432-2242
    Keywords: Fluorescent chromosome banding patterns ; Phaseolus ; Vigna ; Phylogenetic relationships
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Phylogenetic relationships among eight taxa of seven species of Phaseolus and Vigna (Phaseolus angularis, P. aureus, P. calcaratus, P. coccineus, P. vulgaris, Vigna sesquipedalis and V. sinensis; 2n = 22 each) were studied by the fluorescent chromosome banding technique. Preparations of somatic metaphase chromosomes of each taxon were sequentially stained with Giemsa, GC-specific fluorochrome chromomycin A3 (CMA) and AT-specific fluorochrome 4′-6-diamidino-2-phenylindole (DAPI). On the basis of the fluorescent banding patterns of the 22 chromosomes of each taxon, P. angularis, P. coccineus (from China and Korea) and P. vulgaris were grouped into one group (“Phaseolus group”), P. aureus and two Vigna species were grouped into another (“Vigna group”) and P. calcaratus was grouped in an independent group.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223741
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    Paper (German National Licenses)
    Fulltext
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