ISSN:
1437-7780
Keywords:
Key wordsPseudomonas aeruginosa
;
parC mutations
;
Fluoroquinolone resistance
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract To detect quinolone resistance-associated mutations within the Ser-80 and Glu-84 codons of the Pseudomonas aeruginosa parC gene, we developed a rapid and simple assay based on polymerase chain reaction (PCR) amplification of the region of the parC gene containing the mutation sites and digestion of the PCR products with a restriction enzyme. The mutations generating alterations at Ser-80 and Glu-84 were detected as restriction fragment length polymorphisms of the PCR products digested with HinfI. Among 22 clinical isolates tested by this assay, mutations at the Ser-80 and Glu-84 codons were detected in all 10 isolates in which the presence of the mutations had been confirmed previously by DNA sequencing. This rapid and simple assay could be a useful screening device for genetic alterations associated with resistance to quinolones in the P. aeruginosa parC gene.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s101560050045
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